[Histonet] P 16
Has anyone ever used P16 with AP Red detection on the Ventana Benchmark? Phyllis Jordan HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cryostat Feedback Needed (UNCLASSIFIED)
Hi Diane, We have been happy with the Leica cryostats over the years. We have an 1800 that is about 15 years old, an 1850 that is about 5 years old with the UV light for decontamination. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From: Gladney, Diane C Ms CIV USA MEDCOM MACH diane.glad...@us.army.mil To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Wed, March 30, 2011 7:50:23 AM Subject: [Histonet] Cryostat Feedback Needed (UNCLASSIFIED) Classification: UNCLASSIFIED Caveats: NONE Dear Histonetters, Our 11 year old cryostat is beginning to experience some unusual problems. Our Medical Maintenance says that they won't put a lot of money into fixing it because of the age of the unit. Therefore, I am asking for opinions, pros, cons of different brands of cryostats. I am interested in a cryostat that has the UV light or other source of easy decontamination. We don't do a lot of frozen sections but enough for us to have to have a good cryostat that is easy to operate and clean. Any comments or suggestions would be greatly appreciated. Thanks, Diane Gladney Diane C. Gladney, HT (ASCP) Histology Supervisor Department of Pathology Moncrief Army Community Hospital 4500 Stuart Street FT. Jackson, SC 29207 Phone: 803-751-2530 Fax: 803-751-7829 Classification: UNCLASSIFIED Caveats: NONE ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] to microwave or not to microwave
Hi Karla, We chose the Sakura XPress microwave processor. We have had it now for over two years. We eliminated an entire night shift (everyone works day shift now), we drastically reduced xylene usage (we only use it on the HE stainer, we use none for processing anymore). The processing system takes about 1 hour and 30 minutes to process. I can run breast tissue and GI biopsies on the same run. It is still truly amazing to me sometimes. We have cassettes coming off every 40 minutes to an hour now. The techs rotate so that we still have time to do special stains, IHC, frozens...etc. It has been a life saver for us. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From: Karla Sendelbach karla.sendelb...@thedacare.org To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Mon, March 28, 2011 12:44:02 PM Subject: [Histonet] to microwave or not to microwave We are evaluating rapid tissue processing instruments. I would appreciate any comments about microwave tissue process and rapid non microwave tissue process. Peloris II, STP 420ES and Milestone Pathos come to mind. Also, is there anyone who switched from microwave processing to rapid non microwave tissue processing? Thank you, Dr. Sendelbach Appleton Medical Center Appleton Wisconsin 920.738.6294 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Anniston, AL
Hello, I am trying to help a young lady in the Anniston area. She is interested in Histology and would like to try and find a job so that she can train and do one of the online courses. Any contacts for her would be very helpful. Thanks! Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] p16ink4a
Hi All, Is anyone using this P16INK4A for IHC? I searched the archives and found some threads from 2009 with links that do not work for possible suppliers. I also found some for ELISA and Western Blot, and Flow Cytometry. Is this antibody used at all in FFPE tissue? Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Happy Histo Day
Happy Histotechnology Day EveryoneI want to pass this on from Sharon Wehman of Sakura Finetek. Thank you Sharon! It gives me great pleasure to celebrate this day and give thanks to the wonderful histotechs! -Thank you for dedicating your expertise and knowledge to every specimen, block, slide and stain -Thank you for being a part of and making a difference in every diagnosis -Thank you for working hard every day and going above and beyond on most days -Thank you for the incredible care and consideration you give to every patient, even though most do not realize what you do--that is EXCELLENCE! Excellence is never an accident; it is the result of high intention, sincere effort, intelligent direction, skillful execution and the vision to see obstacles as opportunities. May Your Histotechnology Day Be As Wonderful As You Are! Sharon Wehman Area Manager Sakura Finetek USA, Inc. 1750 West 214th Street Torrance, CA 90501 Office:800-725-8723 X:7274 Cell:678-462-6349 sweh...@sakuraus.com www.sakuraus.com Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Distance Learning
We are thinking of using one of the online or distance learning programs to help in the training of new histotechs. Has anyone had any experience with any of these programs, what colleges offer the program, pros, cons. Any feedback is welcome. Thanks Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Optimal processing for prostate needle biopsies
Nilesh, This doesn't sound like a staining problem, it sounds like a processing problem. Try preprocessing for 5 minutes instead of 15 and see if that helps. OR what we had to do with GI biopsies, we stopped using any preprocessing with them. After fixation, they are placed in molecular fixative for 10 minutes before processing. If that doesn't work, I would investigate further to where the biopsy was actually obtained. We had a problem with urologists obtaining the core, then swishing the needle gun around in a container of saline, then ADDING formalin to the saline after the biopsy was obtained. The biopsies were horrible. Hope this helps. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From: Gupta, Nilesh ngup...@hfhs.org To: Phyllis Thaxton dch...@yahoo.com; histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Thu, October 14, 2010 8:08:17 AM Subject: RE: [Histonet] Optimal processing for prostate needle biopsies Phyllis, We hold all our needle cores overnight and pre-process for 15 min. The problems we are having is shrunken nuclei and nucleoli are not distinct even in carcinoma cases. Cracking artifacts of cores (longitudinal), darker and paler staining along the length of the cores. We tried Harris but staining with Mayer's brings out nucleoli better. From: Phyllis Thaxton [dch...@yahoo.com] Sent: Wednesday, October 13, 2010 10:37 AM To: Gupta, Nilesh; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Optimal processing for prostate needle biopsies How long are you pre-processing the prostate biopsies before processing on the XPress120? We use the XPress50 we fix the biopsies in Hollandes for 2 hours, then wash for 20 minutes, pre-processing solution for 10 minutes then process. We use Harris Hematoxylin in our HE and morphology is good. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From: Gupta, Nilesh ngup...@hfhs.org To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Tue, October 12, 2010 12:02:23 PM Subject: [Histonet] Optimal processing for prostate needle biopsies I have a few questions regrading processing of prostate needle biopsies. 1. What is optimal fixation time that the needle cores should be fixed for before loading these on the processors. 2. Our cores are processed on Tissue tek Xpress x120 but the morphology is not so good. I'd like to know if any other labs have tried this processor for prostate needle cores processing and would like to know their experience as far as morphologic quality on slides 3. What is the best HE stain to use for prostate needle biopsies. We are currently using Mayer's which is giving us better staining than Harris. Any recommendations on which HE is best suited for prostate needle biopsies. Thanks N.Gupta Henry Ford Hospital == CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. == ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet == CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number
Re: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR
We run a weekend (Friday til Monday AM) breast run where the tissues are in 10% NBF for 8 hours, then in 70% alcohol for 48 hours in order to complete processing on Monday morning. So far no problems. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From: Kuhnla, Melissa melissa.kuh...@chsli.org To: Mahoney,Janice A janice.maho...@alegent.org; Mike Pence mpe...@grhs.net; Joyce Cline joyce.cl...@wchsys.org; histonet@lists.utsouthwestern.edu Sent: Tue, October 12, 2010 12:02:32 PM Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I disagree. Prolonged formalin fixation (over 48 hrs), diminishes signals -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 12:05 PM To: 'Mike Pence'; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR Formalin fixation time does not impact the results of FISH as it does IHC. Jan M Omaha -Original Message- From: Mike Pence [mailto:mpe...@grhs.net] Sent: Tuesday, October 12, 2010 11:00 AM To: Mahoney,Janice A; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I don't think it matters if you do Her2 by FISH or IHC the time is still 48hr. I hope I am wrong, but I don't think I am. Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 10:25 AM To: 'Joyce Cline'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We have decided to reflex to FISH those breasts that do not fall within the recommended formalin fixation time. We do work on Saturdays so it is only the rare 3 day weekends that this comes into play. Jan M Omaha -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Tuesday, October 12, 2010 10:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR Does anyone have any experience with storing formalin fixed breast tissue in 70% before processing? I am trying to comply with the new guidelines set forth by CAP and ASCO with regard to Her2 and ER/PR and since my lab does not operate on the weekend we have been well above the 48 hour recommended formalin fixation time. Does 70% affect antigenicity for either Her2 or ER/PR? Any information or suggestions will be greatly appreciated. Thanks :) Ronda Souders Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ronda.soud...@wchsys.orgmailto:ronda.soud...@wchsys.org * CONFIDENTIALITY NOTICE * This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please
Re: [Histonet] Optimal processing for prostate needle biopsies
How long are you pre-processing the prostate biopsies before processing on the XPress120? We use the XPress50 we fix the biopsies in Hollandes for 2 hours, then wash for 20 minutes, pre-processing solution for 10 minutes then process. We use Harris Hematoxylin in our HE and morphology is good. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From: Gupta, Nilesh ngup...@hfhs.org To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Tue, October 12, 2010 12:02:23 PM Subject: [Histonet] Optimal processing for prostate needle biopsies I have a few questions regrading processing of prostate needle biopsies. 1. What is optimal fixation time that the needle cores should be fixed for before loading these on the processors. 2. Our cores are processed on Tissue tek Xpress x120 but the morphology is not so good. I'd like to know if any other labs have tried this processor for prostate needle cores processing and would like to know their experience as far as morphologic quality on slides 3. What is the best HE stain to use for prostate needle biopsies. We are currently using Mayer's which is giving us better staining than Harris. Any recommendations on which HE is best suited for prostate needle biopsies. Thanks N.Gupta Henry Ford Hospital == CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. == ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR
Rene, 8 hours is what is on the processor. We keep a log book on all breast tissue. The OR nurse documents on the label; the time of excision (placed in 10% NBF) . The gross assistant documents the time of dissection, Then the time the tissues are placed on the processor. Our fixation times for breast average about 12 to 14 hours. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From: Rene J Buesa rjbu...@yahoo.com To: MelissaKuhnla melissa.kuh...@chsli.org; Janice AMahoney janice.maho...@alegent.org; Mike Pence mpe...@grhs.net; Joyce Cline joyce.cl...@wchsys.org; histonet@lists.utsouthwestern.edu; Phyllis Thaxton dch...@yahoo.com Sent: Wed, October 13, 2010 9:39:21 AM Subject: Re: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR Phyllis: The time required in NBF is not 8 hours only, that is exactly what the regulations are trying to avoid, i.e. incomplete fixation. René J. --- On Wed, 10/13/10, Phyllis Thaxton dch...@yahoo.com wrote: From: Phyllis Thaxton dch...@yahoo.com Subject: Re: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR To: Kuhnla, Melissa melissa.kuh...@chsli.org, Mahoney,Janice A janice.maho...@alegent.org, Mike Pence mpe...@grhs.net, Joyce Cline joyce.cl...@wchsys.org, histonet@lists.utsouthwestern.edu Date: Wednesday, October 13, 2010, 10:32 AM We run a weekend (Friday til Monday AM) breast run where the tissues are in 10% NBF for 8 hours, then in 70% alcohol for 48 hours in order to complete processing on Monday morning. So far no problems. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From: Kuhnla, Melissa melissa.kuh...@chsli.org To: Mahoney,Janice A janice.maho...@alegent.org; Mike Pence mpe...@grhs.net; Joyce Cline joyce.cl...@wchsys.org; histonet@lists.utsouthwestern.edu Sent: Tue, October 12, 2010 12:02:32 PM Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I disagree. Prolonged formalin fixation (over 48 hrs), diminishes signals -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 12:05 PM To: 'Mike Pence'; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR Formalin fixation time does not impact the results of FISH as it does IHC. Jan M Omaha -Original Message- From: Mike Pence [mailto:mpe...@grhs.net] Sent: Tuesday, October 12, 2010 11:00 AM To: Mahoney,Janice A; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I don't think it matters if you do Her2 by FISH or IHC the time is still 48hr. I hope I am wrong, but I don't think I am. Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 10:25 AM To: 'Joyce Cline'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We have decided to reflex to FISH those breasts that do not fall within the recommended formalin fixation time. We do work on Saturdays so it is only the rare 3 day weekends that this comes into play. Jan M Omaha -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Tuesday, October 12, 2010 10:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR Does anyone have any experience with storing formalin fixed breast tissue in 70% before processing? I am trying to comply with the new guidelines set forth by CAP and ASCO with regard to Her2 and ER/PR and since my lab does not operate on the weekend we have been well above the 48 hour recommended formalin fixation time. Does 70% affect antigenicity for either Her2 or ER/PR? Any information or suggestions will be greatly appreciated. Thanks :) Ronda Souders Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ronda.soud...@wchsys.orgmailto:ronda.soud...@wchsys.org * CONFIDENTIALITY NOTICE * This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit
[Histonet] HistoTech Position In Tuscaloosa
FINALLY another HistoTech position has been approved at DCH Regional Medical Center in Tuscaloosa, Alabama. We are a not for profit facility in West Alabama about 50 miles from Birmingham, about 4 hours from the Gulf of Mexico, and about 3 hours from Atlanta. We process about 15000 surgicals per year using Sakura VIP conventional processor, and Sakura ExPress 50 Rapid Tissue Processor, Sakura Prisma HE Stainer with tape coverslipper and Ventana IHC automation. Hopefully we will be automated in Special Stains after October 1. Interested candidates must be proficient in embedding, microtomy, frozen sections, and (for now) manual special staining. Please contact Michelle Fagin at 205-759-7762 or Fax 205-750-5224 OR Sherrie Faulkner at 205-750-5736 or email mfa...@dchsystem.com Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PA in Ohio
Once again, would the recruiter for the PA in Ohio please contact me again. I forwarded your last email then deleted everything. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Alabama Histology Position
FINALLY another HistoTech position has been approved at DCH Regional Medical Center in Tuscaloosa, Alabama. We are a not for profit facility in West Alabama about 50 miles from Birmingham, about 4 hours from the Gulf of Mexico, and about 3 hours from Atlanta. We process about 15000 surgicals per year using Sakura VIP conventional processor, and Sakura ExPress 50 Rapid Tissue Processor, Sakura Prisma HE Stainer with tape coverslipper and Ventana IHC automation. Hopefully we will be automated in Special Stains after October 1. Interested candidates must be proficient in embedding, microtomy, frozen sections, and (for now) manual special staining. Please contact Michelle Fagin at 205-759-7762 or Fax 205-750-5224 OR Sherrie Faulkner at 205-750-5736 or email mfa...@dchsystem.com Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Histology stainers
Hi Michelle, We retired our old Jung about 5 or 6 years ago and bought a Sakura Prisma with the linking tape coverslipper (they have them now with glass coverslips now I think). It has been great, unlike the older coverslippers, this one doesn't use the conveyor belt so there are no slide pile-ups. This is a complete walk away system. Cut slides, put them on and the stainer does the rest. You put them on, then take them off after they are coverslipped. We do some special stains on it as well as HE, easy to program (touch screen)...And like the old Jung, the Prisma IS a workhorse. We retired our Excelsior Processor for The Sakura XPress50. The advantages of the Excelsior is the reagent rotation (just change out bottles of reagent and paraffin and the Excelsior rotates them). We switched to the XPress because of shift staffing problems and our desire to eliminate as much xylene usage as possible. We solved our staffing problems (eliminated the need for a second shift that was necessary with conventional processing), and now all the xylene we use is on the HE stainerHUGE reduction. We still use the old VIP for specimens that come in late that require longer fixation time. We put them on a delay program to come off the following day. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From: Michelle MacVeigh-Aloni macve...@usc.edu To: histonet@lists.utsouthwestern.edu Sent: Thu, September 9, 2010 4:47:29 PM Subject: [Histonet] Histology stainers Hi histoneters, We have an old Jung Autostainer XL, which has been great, but it is very old and on its way out. What kind of newer stainers are you guys using? Should I even look at something diferent? I also would like to hear from the people using Exelsior tissue processor. Do you guys like it and how does it compare to the good old VIP? Michelle Aloni MS HTL ASCP Research Specialist USC Keck School of Medicine LA, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PA
Would the recruiter that has a PA in Ohio that wants to move South, please resend, the email to me with the info...thanks! Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology Position In Alabama
FINALLY another HistoTech position has been approved at DCH Regional Medical Center in Tuscaloosa, Alabama. We are a not for profit facility in West Alabama about 50 miles from Birmingham, about 4 hours from the Gulf of Mexico, and about 3 hours from Atlanta. We process about 15000 surgicals per year using Sakura VIP conventional processor, and Sakura ExPress 50 Rapid Tissue Processor, Sakura Prisma HE Stainer with tape coverslipper and Ventana IHC automation. Hopefully we will be automated in Special Stains after October 1. Interested candidates must be proficient in embedding, microtomy, frozen sections, and (for now) manual special staining. Please contact Michelle Fagin at 205-759-7762 or Fax 205-750-5224 OR Sherrie Faulkner at 205-750-5736 or email mfa...@dchsystem.com Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Full Time Position - Alabama
At DCH we have a full time HistoTech position. Flexible day shift hours Monday through Friday. Must be ASCP registered HT or HTL (or eligible...must become registered within 18 months of hire date). Lab Automation includes IHC stains (Ventana), staining and coverslipping (TissueTek Prisma). We have Leica 2030 microtomes, Sakura embedding centers, Sakura XPress 50 rapid tissue processor, Leica cryostats. Duties include embedding, microtomy, ihc and special stains, and frozen sections. DCH is a 500 plus bed regional medical center. We process around 12,000 to 13,000 specimens annually. Interested candidates please contact Sherrie Faulkner (recruiter) at 205-750-5376 or fax resume to Michelle Fagin at 205-750-5224. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Full Time Day Shift Position
At DCH we have a full time HistoTech position. Flexible day shift hours Monday through Friday. Must be ASCP registered HT or HTL (or eligible...must become registered within 18 months of hire date). Lab Automation includes IHC stains (Ventana), staining and coverslipping (TissueTek Prisma). We have Leica 2030 microtomes, Sakura embedding centers, Sakura XPress 50 rapid tissue processor, Leica cryostats. Duties include embedding, microtomy, ihc and special stains, and frozen sections. DCH is a 500 plus bed regional medical center. We process around 12,000 to 13,000 specimens annually. Interested candidates please contact Sherrie Faulkner (recruiter) at 205-750-5376 or fax resume to Michelle Fagin at 205-750-5224. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
Full Time Day Shift DCH Regional Medical Center Tuscaloosa, Alabama Thu, March 4, 2010 2:35:28 PM From: Phyllis Thaxton dch...@yahoo.com Add to Contacts To: histonet@lists.utsouthwestern.edu At DCH we have a full time HistoTech position. Flexible day shift hours Monday through Friday. Must be ASCP registered HT or HTL (or eligible...must become registered within 18 months of hire date). Lab Automation includes IHC stains (Ventana), staining and coverslipping (TissueTek Prisma). We have Leica 2030 microtomes, Sakura embedding centers, Sakura XPress 50 rapid tissue processor, Leica cryostats. Duties include embedding, microtomy, ihc, special stains, and frozen sections. DCH is a 500 plus bed regional medical center. We process around 12,000 to 13,000 specimens annually. Interested candidates please contact Sherrie Faulkner (recruiter) at 205-750-5376 or fax resume to Michelle Fagin at 205-750-5224. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Full Time Day Shift DCH Regional Medical Center Tuscaloosa, Alabama
At DCH we have a full time HistoTech position. Flexible day shift hours Monday through Friday. Must be ASCP registered HT or HTL (or eligible...must become registered within 18 months of hire date). Lab Automation includes IHC stains (Ventana), staining and coverslipping (TissueTek Prisma). We have Leica 2030 microtomes, Sakura embedding centers, Sakura XPress 50 rapid tissue processor, Leica cryostats. Duties include embedding, microtomy, ihc and special stains, and frozen sections. DCH is a 500 plus bed regional medical center. We process around 12,000 to 13,000 specimens annually. Interested candidates please contact Sherrie Faulkner (recruiter) at 205-750-5376 or fax resume to Michelle Fagin at 205-750-5224. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HE stainer/coverslippers
I am glad you decided on that one Paula. It is wonderful Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From: Paula Lucas plu...@biopath.org To: Liz Chlipala l...@premierlab.com; histonet@lists.utsouthwestern.edu; VickiGauch gau...@mail.amc.edu Sent: Wed, March 3, 2010 10:50:06 AM Subject: RE: [Histonet] HE stainer/coverslippers I second the Prisma with attached coverslipper. We have been using it now for the past 2 months or so. It's really a non-hassle, state of the art piece of equipment. Paula -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Tuesday, March 02, 2010 12:50 PM To: histonet@lists.utsouthwestern.edu; VickiGauch Subject: RE: [Histonet] HE stainer/coverslippers We just purchased the sakura prisma and attached glass coverslipper. We just love them. We tried a lot of glass coverslippers and this by far is the best one that we have tried. We run both HE and specials on the prisma and have not had a problem with the glass coverslipper. We have been using them for about a little over 2 months now. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, March 02, 2010 1:39 PM To: histonet@lists.utsouthwestern.edu; VickiGauch Subject: Re: [Histonet] HE stainer/coverslippers Sakura René J. --- On Tue, 3/2/10, Gauch, Vicki gau...@mail.amc.edu wrote: From: Gauch, Vicki gau...@mail.amc.edu Subject: [Histonet] HE stainer/coverslippers To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Tuesday, March 2, 2010, 2:20 PM Hi, I was wondering what type of HE stainer/coverslipper units labs are using and the pros and cons of each. We found out we have funding to replace our old faithful GLX stainer(though it will break my heart to do so) and are looking for a stainer/ coverslipper that is one unit- one that we do not have to unload from the stainer to load onto the coverslipper. Any help would be greatly appreciated. Thanks so much, Vicki Gauch AMCH Albany,NY - CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HistoTech Position at DCH Regional in Tuscaloosa, AL
At DCH we have a full time HistoTech position. Flexible day shift hours Monday through Friday. Must be ASCP registered HT or HTL. Lab Automation in the lab includes IHC stains (Ventana), staining and coverslipping (TissueTek Prisma). We have Leica 2030 microtomes, Sakura embedding centers, Sakura XPress 50 rapid tissue processor, Leica cryostats. DCH is a 500 plus bed regional medical center. Interested candidates please contact Sherrie Faulkner (recruiter) at 205-750-5376 or fax resume to Michelle Fagin at 205-750-5224. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HistoTech Position At DCH In Tuscaloosa, Alabama (Phone contact correction)
At DCH we have a full time HistoTech position. Flexible day shift hours Monday through Friday. Must be ASCP registered HT or HTL. Lab Automation in the lab includes IHC stains (Ventana), staining and coverslipping (TissueTek Prisma). We have Leica 2030 microtomes, Sakura embedding centers, Sakura XPress 50 rapid tissue processor, Leica cryostats. DCH is a 500 plus bed regional medical center. Interested candidates please contact Sherrie Faulkner (recruiter) at 205-750-5736 or fax resume to Michelle Fagin at 205-750-5224. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Robotic Prostatectomy
For the first time here, we have Urologists using the robotic method for removing prostates. The PA always receives them fresh, inks them, then puts the whole prostate in alcoholic fixative (currently Zfix from Anatech). The next day they are grossed in and processed. The prostates we have received lately are raw in the middle and unable to be grossed in, whereas before they were fine. Has anyone had similar experiences? Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PIN 4
I went to archives to look for this but I didn't get a definite answer to my specific question. I am using Biocare's Prostate Cocktail - 2X (CK5 + CK14 + p63) and Biocare's P504S-2X on the Ventana platform using UltraView DAB and Ultra View RED kits. To code this procedure, is it 88342 X 3 or 88342 X 4? I would like some rationale as well. Thanks! Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Michael LaFriniere
If anyone knows how to contact Michael, please email me. Thanks! Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Formula 83
Does anyone have any experience with Formula 83. We are evaluation it now and I would like to know how frequently it needs to be changed on the processor. The label says use just like xylene...BUT is there anyone out in HistoLand that uses it? I would like to know your experience in processing. Right now we are using it on a VIP. Thanks!!! Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] NSH Meeting in Alabama
I agree Michael!!! Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From: mtitf...@aol.com mtitf...@aol.com To: histonet@lists.utsouthwestern.edu Sent: Friday, July 17, 2009 10:33:34 AM Subject: [Histonet] NSH Meeting in Alabama With the NSH Annual Convention in Alabama this year, I don't want any corny jokes on the Histonet?about my adopted home state!? Might hurt my feelings! Michael Titford Pathology USA Mobile AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] VALIDATION FOR FIXATIVES
Does anyone out there know any reference labs that do egfr, her2 fish, k ras gene mutation, etc. that have performed validation testing for tissues fixed in anything other than 10% NBF? Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Frozen Section TAT
Hi All, I haven't been on in a couple of weeks, but thank all of you for the discussion on FS turnaround time reporting. I thought we had everything in place, but one thing we didn't even consider is when breast biopsies are send for radiology before arriving for FS. We are also reporting receiving time as order time because they come in a pneumatic zip tube in -60 seconds. I guess if there is more than one room trying to send and are in line waiting to be zipped, then the order time would be inaccurate the way we are reporting it. Thanks all!! Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From: Lott, Robert robert.l...@trinitymedicalonline.com To: histonet@lists.utsouthwestern.edu Sent: Friday, June 26, 2009 11:00:27 AM Subject: [Histonet] Frozen Section TAT Becky Garrison makes some very valid and interesting points below First of all, the point about what a critical test is, as defined by JCHAO. She is correct, in that these are tests defined by the individual institution in policy, but not by JCAHO. Second, the item (ANP.11820) on the CAP checklist concerning turnaround time of intraoperative frozen sections reads as follows: The institution must periodically evaluate TAT and document reasons for delay BUT only if 90% of FS are not completed within 20 minutes. ANP.11820 Phase 1 Does the laboratory periodically evaluate turnaround time for intraoperative frozen sections? NOTE: If 90% of frozen sections are not completed within 20 minutes, the laboratory must document evaluation of the reason(s) for the delay. This turnaround time is intended to apply to the typical single frozen section. In cases where there are multiple sequential frozen sections required on a single specimen (e.g., resection margins), or in cases where additional studies such as radiographic correlation are required, longer turnaround times may be expected. COMMENTARY: N/A REFERENCE: Novis DA, Zarbo RJ. Interinstitutional comparison of frozen section turnaround time. A College of American Pathologists Q-Probes study of 32,868 frozen sections in 700 hospitals. Arch Pathol Lab Med. 1997;121:559-567. Robert L. Lott, HTL(ASCP) / Manager, Anatomic Pathology Trinity Medical Center/ LabFirst/ 800 Montclair Road / Birmingham, AL 35213/ 205-592-5387 Message: 19 Date: Thu, 25 Jun 2009 10:49:43 -0400 From: Garrison, Becky becky.garri...@jax.ufl.edu Subject: RE: [Histonet] Re: tracking turnaround time of intraoperativeconsultations To: Della Speranza, Vinnie del...@musc.edu, Robert Richmond rsrichm...@gmail.com, histonet@lists.utsouthwestern.edu Message-ID: b3ca1baf6c5e4541867e4e79ad2412e003c29...@jaxmail.umc.ufl.edu Content-Type: text/plain; charset=us-ascii Sorry I did not respond yesterday. The reason we just started measuring order to sign out for frozens was also prompted by preparation for our next JCAHO inspection. However, there is this distinction. The Joint Commission does not define a frozen as a critical test. The designation of critical test is left up to the individual institution. However, once your institution defines the frozen as a critical test, (indicated somewhere in a policy), you must conform to JCAHO guidelines for critical tests. And apparently this is the buzz with JCAHO watchers right now. Here we designate the IntraOp consultations for frozens (not gross only or Touch prep) and IntraOp PTH (Clinical test) as a critical tests and have started tracking order to sign out. Order time is the time the surgeon indicates 'send this to pathology' not when pathology receives the specimen. We are somewhat in uncharted waters as there is no national standard that defines target time from order to sign out. We set a 40 minute time (20-OR to Path and 20-path to completed frozen). I campaigned against a 30 minute total time (15 each) because we do have some frozens that do take over 15 minutes and this was an absolute value (unlike the CAP goal of 90% within 20 minutes). Our approach is to monitor, evaluate the data we retrieve. There will certainly be adjustments made to target time and how and what we monitor. The data collection raises more questions: how do you come up with meaningful data for multiple specimens on a single case; multiple frozens (different patients) received together or before we are finished with the first patient's frozen). This is one of those ideas that sounds good in theory but presents some challenges in execution. But it is a valid process to monitor as we periodically have surgeons complain of the time they are waiting for frozen results. This is really a joint quality management review which involves multiple departments (OR and Pathology) and how we make it better for the patient. Becky Garrison Pathology Supervisor Shands
[Histonet] Cardboard Paraffin Catchers
Does anyone remember the little cardboard microtome trays for paraffin waste? If so please email me the company that makes them. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] AMMONIUM HYDROXIDE
Hi all, I looked in archives to see if I could find any info on this but did not. Remember, we are having a problem with some of the sections floating off during IHC. If paraffin blocks are faced and then placed in ammonium hydroxide before cutting the HE, would this cause the sections to float off if the unstained sections that are cut in addition to the HE's are also produced in this manner? Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Many Thanks
I think our problem may very well be the slides. We tried many slides in the past and always came back to the superfrost brand that was most reliable, but I have found different opinions on these slides ;ately. I think it is time to change brands. Thank you all...Hope to see everyone in Birmingham Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Benchmark XT
Is anyone out there having problems with tissue washing off the slides using the Ultraview kit from Ventana? Mainly we are having problems with needle biopsies washing off (prostate, liver). Fixation and processing is always the same 4-6 hours fixation, 3 hour biopsy processing run on VIP processor, cut no thicker than 4 microns, airdried at least 30 minutes, baked at 59-60 for at least one hour prior to staining. The instrument's calibrations have been checked and are fine. We never had this problem with the iView kit using the Nexes, doing pretreatments offline. Any help, ideas, info will be appreciated. Thanks, Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL 35401 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Michael LaFriniere
Please give me a call stranger...205-759-7487 Phyllis ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Michael LaFriniere
please call me at work 205-759-7487 Phyllis ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
