I would appreciate help with finding a good paraffin protocol for drosophila
(fruit flies). I am currently using Bouin's fixative followed by a routine
alcohol, xylene substitute and paraffin infiltration technique. I need a
protocol that will allow better infiltration than I am currently getting, I
have tried potassium hydroxide to soften the outer layer of the fruit fly but
there was only a slight improvement. We are looking at the Malpighian tubules
and I will need serial section.
thanks
Linda Prentice
University of California, SF
Preventive and Restorative Dental Sciences.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet