[Histonet] HHV8 Antibody
Has anyone had experience with this antibody? One of our Hemepaths wants to bring this on line. Where do you get your antibody and what is your protocol? Any info would be much appreciated. Rae Staskiewicz UnityPoint Health-Methodist/Proctor ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] C-Myc
Would anyone have a protocol for C-Myc using the Ventana Benchmark Ultra or XT? Thanks in advance for any information you can provide. Rae Ann Staskiewicz UnityPoint Health Methodist Peoria, IL 309-672-5994 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] H pylori and Ventana instruments
Hi all, Those of you using Ventana instrumentation, could you tell me what H pylori antibody you are using, and whether or not you are experiencing non-specific staining, or staining that looks specific but with a stippled effect. Thanks Rae Staskiewicz UnityPoint Health-Methodist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FW: processing after Bouin's fixation
Sending this for a friend, please do not reply to me. _ From: Burton, Lynn [mailto:lynn.bur...@illinois.gov] Sent: Thursday, October 18, 2012 8:43 AM To: raest...@grics.net Subject: FW: processing after Bouin's fixation Rae, I have been trying to get this out on Histonet and I can't seem to address it correctly. It is for Dr. Dybas. Would you please help me? Lynn From: Burton, Lynn Sent: Wednesday, October 17, 2012 3:14 PM To: 'histo...@lists.utsouthwester.edu'; 'histonet-boun...@lists.utsouthwestern.edu' Subject: FW: processing after Bouin's fixation From: Burton, Lynn Sent: Wednesday, October 17, 2012 1:46 PM To: 'on behalf of Lynn Burton' Subject: processing after Bouin's fixation Hi, I have a question about the step in between Bouin's fixation and alcohol dehydration of rat brain tissue, nucleus accumbans. Several procedures are suggested by various resources, what are any of you using successfully? Lynn Burton Animal Disease Lab Galesburg,Il ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ADH 5
Is anyone doing the ADH 5 stain on a Ventana platform? Rae Staskiewicz Methodist Medical Center in Illinois ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] QIHC
Is there any other resource for information and studying for the QIHC exam other than the ASCP outline? Rae Staskiewicz ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] H Pylori Control
Good Afternoon everyone, My institution is about to start running H Pylori for another lab. They microwave process their tissue. Would there be anyone out there who microwave processes and would have one or two blocks of H Pylori positive control tissue they would be willing to let us have? Thanks in advance, Rae Ann Staskiewicz Methodist Medical Center of Illinois Peoria, IL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] AMPHYL
Linda, Where do you get your Lysol IC? Rae Staskiewicz -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Thursday, August 25, 2011 1:07 PM To: barbara.cr...@lpnt.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] AMPHYL We use Lysol I. C.; haven't be able to get Amphyl for years. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of barbara.cr...@lpnt.net Sent: Thursday, August 25, 2011 12:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AMPHYL My materials management department told me that AMPHYL has been discontinued. Ventana recommends that we clean/decontaminate the Benchmark and the Ultras with AMPHYL. Has anyone else ran across this? Has AMPHYL really been discontinued? Is there a substitute we can use. Thanks everyone! ANTOINETTE CRILL ANATOMIC PATHOLOGY EXT 5451 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Outdating- Embedding Wax and Mounting Media
Now you guys have done it!!! All the QAQC gurus are thinking!!! Rae Staskiewicz -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Saturday, May 14, 2011 4:56 PM To: John Smallwood; Histonet Subject: Re: [Histonet] Outdating- Embedding Wax and Mounting Media How funny! You are so right! What about the ink on all of those old reports and reference books? Isn't ink a dye in solvent? The best special stains I ever did were back in the day when I made my own solutions from dry chemicals that were already 20 to 30 years old. Paperwork, just a way to decrease productivity! Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 631 N Broadway Moore, OK 73160 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com From: John Smallwood jrsmallw...@bell.net To: Histonet Submissions histonet@lists.utsouthwestern.edu Sent: Sat, May 14, 2011 4:18:36 PM Subject: [Histonet] Outdating- Embedding Wax and Mounting Media To all Quality Control Purists There seems to be a growing concern with regards to outdating on reagents and their continued use beyond this date. I notice that Histological wax and Mounting media are dated with an expiry date. Just begging the question-should we go back. melt down and re-embed all old blocks in fresh wax ?? Should we recover slip old slides in fresh dated Mounting media. With all the fuss over quality control in the Laboratory and Government Lab Inspection oversight , how can this be ignored and our archives put at such dreadful risk ?? J. Smallwood,MLT. STEGH St.Thomas, Ont. Can. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Competancies for handling hazardous material
Has anyone been inspected by the EPA regarding removal of hazardous chemicals? It came up in a safety meeting that I should have training and competencies (beyond general safety training and PPE) for pouring xylene from staining dishes and processors into accumulation containers. Rae Ann Staskiewicz ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Contact information
Could anyone send me the contact information for Charlie Doerner? Rae Ann Staskiewicz ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Pax 8
Does anyone know where to purchase Pax 8 antibody for formalin fixed paraffin embedded tissue? Rae Staskiewicz Methodist Medical Center ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cutting Microarrays
Good Morning All, I am having some issues when cutting a microarray containing 4mm punches. The punches are rolling and separating from the paraffin during cutting. Anyone have any tips or ideas?? Rae Staskiewicz MMCI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Lung biopsies - Question
We cut 10 unstained slides. Rae Staskiewicz Methodist Medical Center of Illinois -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, August 26, 2010 3:54 PM To: Histonet Subject: [Histonet] Lung biopsies - Question How many labs cut extra tissue sections (or tissue curls) for molecular testing (KRAS, BRAF, EGFR, etc.) on lung biopsies up front (at the time of HE sectioning)? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Barcode and Tracking Information
Jan, Ditto! Ditto! Ditto! Couldn't have said it better myself! Rae Staskiewicz -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Wednesday, May 05, 2010 2:53 PM To: 'Maggie Allen'; histonet@lists.utsouthwestern.edu Cc: histonet-requ...@lists.utsouthwestern.edu; :histonet-bounces@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: [Histonet] RE: Barcode and Tracking Information Blake, I think I put in my comments about Ventana's Vantage when you first posted the question, but here goes again.. It is wonderful. Vantage is the best thing to come along in my lifetime as a histo tech! Wonderful for patient safety, easy for the techs to use and a manager's dream for all the data it can provide. Jan Mahoney Omaha, NE -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Maggie Allen Sent: Wednesday, May 05, 2010 2:24 PM To: histonet@lists.utsouthwestern.edu Cc: histonet-boun...@lists.utsouthwestern.edu; :histonet-bounces@lists.utsouthwestern.edu; histonet-requ...@lists.utsouthwestern.edu Subject: [Histonet] RE: Barcode and Tracking Information Hello Blake, LabelClinic HTS is a tracking system for Histology artifacts within the Pathology workflow. The product is designed to provide improved quality control and traceability with the lab. It provides flexible and reliable labeling and tracking of histology requisitions, containers, specimens, blocks and slides. Using bar code technology, LabelClinic HTS provides an optimized workflow that reduces errors and significantly enhances patient safety. It can be used stand alone, or integrated into an LIS / AP software system. Benefits: * Flexible lab configuration for custom lab workflow * Reduce errors and increase efficiency * Just-in-time reporting of artifact locations or last know location * Alerting for sub-process time violations and missing artifacts * Deploy to Desktop PC's or mobile hand held's * Configurable workflow * Supports color selection for slide and cassette printers I would be happy to set up an online WebEx demo of the system for anyone who may be interested. Thank you! Maggie Allen Healthcare Business Development Manager Niceware International, LLC 200 South Executive Drive Suite 200 Brookfield, Wisconsin 53005 Tel (810) 629-3930 Cell (215) 200-0268 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Illinois Annual Meeting
This year the Illinois Society for Histotechnologists is holding their Annual Spring meeting at Eaglewood Resort in Itaska, Illinois. The dates are May 13-14, 2010. Unfortunately it seems as though we are trying to follow the example of the Republican National Committee and have an incorrect phone number for room reservations at the resort. The correct number is 1-877-285-6150. Be sure to mention that you are with the ISH when making your reservation in order to receive the convention rates. Reservations must be made by April 12 in order to receive the reduced rates. Check out the Eaglewood Resort at www.eaglewoodresort.com http://www.eaglewoodresort.com/ to see all of the amenities waiting for you! If you would like an electronic brochure, contact myself at raest...@grics.net. Rae Ann Staskiewicz ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Her-2 Controls
Morning All, My chief pathologist has asked the following question. For all of those doing Her-2 Neu staining for scoring with the Vias system. How many control slides do you cut in advance and how long are they viable if kept refrigerated? Thanks in advance, Rae Ann Staskiewicz ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FW: Commercial Source for Direct Immunofluorescence Control Tissue
Forwarding this for someone else. Rae Staskiewicz _ From: Dana Spears [mailto:dspe...@mmci.org] Sent: Monday, April 06, 2009 3:11 PM To: histonet-requ...@lists.utsouthwestern.edu Subject: Commercial Source for Direct Immunofluorescence Control Tissue Anyone know of a commercial source for FITC Control tissue? I've searched the archives and saw the question asked before, but no answer! Thanks to all who respond! Dana Spears, HTL(ASCP) Laboratory Manager Methodist Medical Center (309) 672-4930 (office) (309) 255-7214 (cell) (309) 279-3768 (fax) dspe...@mmci.org NOTICE: This message is a PRIVATE communication. This e-mail may contain confidential or proprietary information that may be considered legally privileged. It is intended only for the named recipient(s). If an addressing or transmission error has misdirected the e-mail, please notify the author by replying to this message. If you are not the named recipient, you are not authorized to use, disclose, distribute, copy, print, or rely on this e-mail, and should immediately delete it from your computer system. Thank you for your assistance with this matter. NOTICE: This message is a PRIVATE communication. This e-mail may contain confidential or proprietary information that may be considered legally privileged. It is intended only for the named recipient(s). If an addressing or transmission error has misdirected the e-mail, please notify the author by replying to this message. If you are not the named recipient, you are not authorized to use, disclose, distribute, copy, print, or rely on this e-mail, and should immediately delete it from your computer system. Thank you for your assistance with this matter. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fall Symposium
The Illinois Society for Histotechnologists is holding a one day seminar on November 8, 2008 at the Four Points by Sheraton in Fairview Heights, IL near St. Louis, MO. Get 2 workshops (6 CEUs) for only $35! Registration includes lunch. You can register on-line at www.ilhisto.org http://www.ilhisto.org/ , or register on the day for only $10.00 additional. Workshops being offered are: Basic Dynamics of Fixation and Processing - Herbert Skip Brown Where Do I Find that Antibody and What Do I Do Once I have it? - Charlie Dorner ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
