[Histonet] Macrophage
I am going to perform a CCRL2 IHC Stain on some mouse lung, my control has already been made and put on a slide, it is a macrophage. Does anybody know how I should prep the macrophage slide for staining? Do I treat it like a cytospin and start it in 95% ETOH then to DI H2O to TBS? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Antibody deposit visualization
One of my clients has been performing experiments with a mouse model of Dengue Virus Infection. They have been treating the mice with monoclonal antibodies (client did not specify what antibody)as a potential treatment for dengue virus infection. They have harvested the kidneys and put them in 10% NBF. They want to know if routine HE will show any antibody deposits. I told him that I would not be able to directly observe the antibody deposit itself, however I might be able to observe the reaction to the antibody deposit in the basement membrane of the glomeruli as a membranous glomerulopathy on an HE. I was wondering if I should do any special stains that might show more than an HE. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
