[Histonet] Tissue Tek Prisma Model 6130

2014-07-11 Thread Rolly Perez
Any experience-based resolution on the problem of the touch screen pad/monitor 
of the Tissue Tek Prisma Stainer 6130 wherein the screen turns black while a 
program is running. The program continues to run even though the screen is not 
illuminated.

I thought I'd throw this out there in case someone knows a way to resolve it 
before I call for repair. I'm still able to use the stainer by placing sticker 
labels on the screen so I know where to press :-)


Rolly Perez
Histology Supervisor | MedSurg Pathology
8192 SW Durham Road, Tigard OR 97224
Tel: 503.443.2157 | Fax: 503.670.9754


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[Histonet] Cytolyt Solution

2014-02-19 Thread Rolly Perez

Anyone knows how to make Cytolyt Solution?



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[Histonet] Cryostat

2012-02-29 Thread Rolly Perez
Anyone interested in a used, working Cryostat, contact me directly:

Leica/Reichert-Jung CRYOCUT 1800

Rolly Perez, HT(ASCP)
Histology Supervisor
MedSurg Pathology Associates, Inc.
Tigard, OR
503.443.2157




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[Histonet] Re-send Darker &/or granular hematoxylin staining (no photo)

2012-02-09 Thread Rolly Perez
Hello my colleagues,

Any input will be much appreciated - what can cause darker and/or granular
hematoxylin staining on the goblet cells (mucin areas) of certain GI
biopsies while on other similar GIs in the very same rack of slides, they
stain water clear which is the preferred outcome.

I use regressive H&E staining using Sigma Aldrich Harris hemtoxylin &
standard 1% Acid alcohol (hydrocholric acid).

I tried stronger acid for differentiation, cut thinner, longer time in
acid differentiation, less hematoxylin time, to try to get the mucin
staining as minimal as possible but I can't seem to eliminate the darker
&/or more granular staining on some GIs.

Thanks in advance for any thoughts you might have!

Rolly Perez, HT(ASCP)
Histology Supervisor
MedSurg Pathology Associates, Inc.
503.443.2157


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