[Histonet] AFB and Spirochetes slides needed

[Sheeder, Christopher via Histonet]

Good morning all,

I am validating new stains and looking for 4-5 slides each of AFB+ and 
Spirochetes+.

I am willing to trade for something you need.

Email me directly if you want to work something out.

Regards-

Christopher Sheeder, HT(ASCP)CMQIHC
Department of Laboratories
Seattle Children's Hospital
4800 Sand Point Way NE
Seattle, WA 98105

Office: 206-987-6259

christopher.shee...@seattlechildrens.org

WWW  seattlechildrens.org
COMPASSION | EXCELLENCE | INTEGRITY | COLLABORATION | EQUITY | INNOVATION

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Re: [Histonet] Rhabdomyosarcoma Control Tissue

[Sheeder, Christopher via Histonet]

Hi Kelly,
I will get them out via FedEx this afternoon.
These are from my own personal stash so they are a gift.
Best,
Chris

Christopher Sheeder, HT(ASCP)CMQIHC
Histology Supervisor | Department of Laboratories
Seattle Children's Hospital
4800 Sand Point Way NE
Seattle, WA 98105
Office: 206-987-6259

christopher.shee...@seattlechildrens.org




Seattle Children's - Internal
From: Pairan, Kelly 
Sent: Tuesday, September 01, 2020 10:58 AM
To: Sheeder, Christopher ; 
'histonet@lists.utsouthwestern.edu' 
Subject: RE: [Histonet] Rhabdomyosarcoma Control Tissue

Hi Chris,
Our address is:
Nationwide Children's Hospital
Anatomic Pathology
700 Childrens Drive
Room C0340
Columbus, OH
43205

Thanks so much for your help.  Do you need anything?

Have a great day!
Kelly

From: Sheeder, Christopher 
mailto:christopher.shee...@seattlechildrens.org>>
Sent: Tuesday, September 01, 2020 1:53 PM
To: Pairan, Kelly 
mailto:kelly.pai...@nationwidechildrens.org>>;
 'histonet@lists.utsouthwestern.edu' 
mailto:histonet@lists.utsouthwestern.edu>>
Subject: RE: [Histonet] Rhabdomyosarcoma Control Tissue

[WARNING: External Email - Use Caution]


Hi Kelly,
Reply with your address and I will send you a couple blocks.
Chris

Christopher Sheeder, HT(ASCP)CMQIHC
Histology Supervisor | Department of Laboratories
Seattle Children's Hospital
4800 Sand Point Way NE
Seattle, WA 98105
Office: 206-987-6259

christopher.shee...@seattlechildrens.org


-Original Message-
From: Pairan, Kelly 
mailto:kelly.pai...@nationwidechildrens.org>>
Sent: Monday, August 31, 2020 12:30 PM
To: 'histonet@lists.utsouthwestern.edu' 
mailto:histonet@lists.utsouthwestern.edu>>
Subject: [Histonet] Rhabdomyosarcoma Control Tissue

Good Afternoon,
We are currently looking for some rhabdomyosarcoma control tissue.  All of our 
cases that have a significant amount of tissue have post treatment necrosis.  
We would be happy to share anything we have that you might need.

Thanks for your help,
Kelly


Seattle Children's - Internal

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Re: [Histonet] Rhabdomyosarcoma Control Tissue

[Sheeder, Christopher via Histonet]

Hi Kelly,
Reply with your address and I will send you a couple blocks.
Chris

Christopher Sheeder, HT(ASCP)CMQIHC
Histology Supervisor | Department of Laboratories
Seattle Children's Hospital
4800 Sand Point Way NE
Seattle, WA 98105
Office: 206-987-6259

christopher.shee...@seattlechildrens.org


-Original Message-
From: Pairan, Kelly 
Sent: Monday, August 31, 2020 12:30 PM
To: 'histonet@lists.utsouthwestern.edu' 
Subject: [Histonet] Rhabdomyosarcoma Control Tissue

Good Afternoon,
We are currently looking for some rhabdomyosarcoma control tissue.  All of our 
cases that have a significant amount of tissue have post treatment necrosis.  
We would be happy to share anything we have that you might need.

Thanks for your help,
Kelly


Seattle Children's - Internal

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Re: [Histonet] Sausage controls

[Sheeder, Christopher via Histonet]

Here is the article...
Multitumor "Sausage" Blocks in Immunohistochemistry Simplified Method of 
Preparation, Practical Uses, and Roles in Quality Assurance
Rodney T. Miller, M.D., Carol L. Groothuis, MT(ASCP)SI
American Journal of Clinical Pathology, Volume 96, Issue 2, 1 August 1991, 
Pages 228-232, https://doi.org/10.1093/ajcp/96.2.228

Christopher Sheeder, HT(ASCP)CMQIHC
Histology Supervisor | Department of Laboratories
Seattle Children's Hospital
4800 Sand Point Way NE
Seattle, WA 98105
Office: 206-987-6259

christopher.shee...@seattlechildrens.org



Seattle Children's - Internal

-Original Message-
From: Mac Donald, Jennifer 
Sent: Wednesday, August 19, 2020 10:30 AM
To: Emily Horst 
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Sausage controls

The Journal of Histotechnology had an article regarding this years ago.  
Perhaps you can find it in the archives.  Dr. Hector Battifora was the author.
Jennifer

-Original Message-
From: Emily Horst via Histonet 
Sent: Wednesday, August 19, 2020 10:11 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Sausage controls

  EXTERNAL SENDER- Exercise caution with requests, links, and attachments.

Hello all,

For those of you who use a sausage control for IHC/special stains, could you 
direct me as to process for starting that instead of using individual controls 
for everything?

Thanks in advance!

Emily
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Re: [Histonet] Warm formalin

[Sheeder, Christopher via Histonet]

How hot are we talking about?
San Francisco hot or Phoenix hot?


Christopher Sheeder, HT(ASCP)CMQIHC
Histology Supervisor | Department of Laboratories
Seattle Children's Hospital
4800 Sand Point Way NE
Seattle, WA 98105
Office: 206-987-6259

christopher.shee...@seattlechildrens.org


-Original Message-
From: Martin, Erin 
Sent: Tuesday, July 21, 2020 6:14 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Warm formalin

Hello everyone!

We have a referring clinician that is concerned about leaving his specimens in 
an outdoor lockbox in the summer because the formalin will get hot.  I don't 
think that having some specimens in formalin in hot weather would cause any 
problems but I can't find any references one way or another.  Does anyone have 
any policies regarding this?

Thanks so much!


Erin Martin, Histology Supervisor

UCSF Dermatopathology & Oral Pathology Service

Phone: 415-3537248 | Fax: 415-353-7543


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Re: [Histonet] Lab assistant/histotech

[Sheeder, Christopher via Histonet]

Hi Samantha,
I have used lab assistants throughout my career. Here is a list of duties they 
would perform:
Accession specimens
File blocks and slides
Clean glassware
Restock supplies
Clean work areas
Fax/scan/print reports
Answer phone
Recycle solvents
Dump tissue
Assist PA's in grossing (make cassettes/document workload)

Have a great Monday!
Chris

Christopher Sheeder, HT(ASCP)CMQIHC
AP Manager | Department of Laboratories
Seattle Children’s Hospital
4800 Sand Point Way NE
Seattle, WA 98105
Office: 206-987-6259

christopher.shee...@seattlechildrens.org


-Original Message-
From: samantha.gol...@hcahealthcare.com 
Sent: Saturday, July 04, 2020 5:29 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Lab assistant/histotech

I was curious if there is a source, publication, report, study, general 
information, regarding the use of lab assistants in the histology lab.

I would like the know the percentage of labs that utilize assistants versus 
labs with techs only. I would also like to know that for labs that do utilize 
assistants, what duties do they perform that the techs are exempt from.

I’m simply curious to see how other labs have workflow setup.

Thanks for any insight.

Samantha


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Re: [Histonet] Flammable Sprays in Cryostats

[Sheeder, Christopher via Histonet]

I would like the link as well, if possible.

Christopher Sheeder, HT(ASCP)QIHC
Histology Supervisor | Department of Laboratories
Seattle Children’s Hospital
4800 Sand Point Way NE
Seattle, WA 98105



-Original Message-
From: jasonhause...@gmail.com 
Sent: Thursday, September 26, 2019 6:01 AM
To: P Sicurello 
Cc: Akemi ; Knutson, Deanne ; 
Histonet 
Subject: Re: [Histonet] Flammable Sprays in Cryostats

I woukd like the link to that. I looked around a bit on the web but couldnt 
locate usinf search.Jason HauserSenior HistotechnologistThe South Bend 
ClinicSent from my T-Mobile 4G LTE device-- Original message--From: P 
SicurelloDate: Thu, Sep 26, 2019 7:56 AMTo: jasonhause...@gmail.com;Cc: 
Akemi;Knutson, Deanne;Histonet;Subject:Re: [Histonet] Flammable Sprays in 
CryostatsThis was copied from the Centers for Disease Control's Morbidity and 
Mortality Weekly Report (January 2012)5.9.2. Work at the open benchFrozen 
sections— Frozen sectioning is performed on fresh tissue and is a high-risk 
procedure for infectious exposure. Freezing tissue does not kill organisms, and 
the use of the cryostat cutting blade creates potentially dangerous aerosols. 
Discuss the true clinical necessity for frozen sectioning with the surgical 
team.— Although some cryostat instruments have a downdraft into the instrument, 
aerosols are dispersed into the room where the cutting takes place. Do not use 
freezing propellant sprays, which speed the freezing process by a few seconds 
and cause aerosolization of not only the tissue being frozen but also the 
tissues from previously cut specimens that are at the base of the instrument. 
Such procedures generate aerosol and droplet contamination, posing an 
infectious risk to all personnel in the area (56,79,82). The Clinical and 
Laboratory Standards Institute and others have recommended discontinuation of 
freezing sprays because they are not recommended by the manufacturers of 
cryostat instrumentation (2,79).  Sincerely,

Paula
Sicurello, HTL (ASCP)CM

Histotechnology
Specialist

UC San Diego Health

9300 Campus Point DriveLa Jolla, CA 92037

(P):
858-249-5610



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error, please contact the sender and delete the material from any computer.On 
Thu, Sep 26, 2019 at 4:37 AM jasonhause...@gmail.com  
wrote:








I have heard this before. Any documention of this actually happening.I have 
tested for blood on surfaces outside the cabinet and never observed a positive 
result.Jason HauserSenior HistotechThe South Bend ClinicSent from my T-Mobile 
4G LTE device-- Original message--From: P SicurelloDate: Wed, Sep 25, 
2019 11:17 PMTo: jasonhause...@gmail.com;Cc: Akemi;Knutson, 
Deanne;Histonet;Subject:Re: [Histonet] Flammable Sprays in CryostatsIt's not 
safe to use any type of spray in a cryostat.  It creates aerosols of who knows 
what (tuberculosis) from potentially infectious patient biopsies.  I would not 
recommend it, just as a universal precaution.Sincerely,

Paula
Sicurello, HTL (ASCP)CM

Histotechnology
Specialist

UC San Diego Health

9300 Campus Point DriveLa Jolla, CA 92037

(P):
858-249-5610



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error, please contact the sender and delete the material from any computer.On 
Wed, Sep 25, 2019 at 7:16 PM jasonhauser71--- via Histonet 
 wrote:There are non flammable sprays 
available. We got the recall notice and switched our old inventory out. I found 
3 different histology specific sprays. The electronic industry offers some as 
well. Another option is just using LN2 .Jason HauserSenior Histology 
TechnicianThe South Bend ClinicSent from my T-Mobile 4G LTE device-- 
Original message--From: AkemiDate: Wed, Sep 25, 2019 10:05 AMTo: Knutson, 
Deanne;Histonet;Cc: Subject:Re: [Histonet] Flammable Sprays in CryostatsWe are 
purchasing two of their newer UV cryostats and they were adamant that we use a 
non flammable freezing spray because it can damage the UV unit and have 
potential for exploding.

Akemi Allison

Sent from my iPhone

> On Sep 25, 2019, at 7:50 AM, Knutson, Deanne via Histonet  wrote:
>
> I just received a letter from Leica Biosystems where they are
> prohibiting the usage of flammable freezing sprays in their 

Re: [Histonet] Help to interview new employees for the first time

[Sheeder, Christopher via Histonet]

Hi Blanca,
Hiring has become more difficult in recent years. Most employers now only 
verify past employment. They cannot divulge any corrective actions, performance 
issues or firings.
References are hand-picked by the applicant so you don't get the whole picture 
there either.
I typically ask them about their experiences in histology. Obstacles they have 
overcome, how to handle difficult customers (angry physicians) etc.
If you can give the other techs a chance to ask the candidate questions, great, 
otherwise see what questions your techs want to know.
I fully agree with Terri Braud's response. Do not have them perform any type of 
function. That is the purpose of the probationary period.
The best interview question that was ever asked of me..."Name 12 uses for a 
pencil".
Seems silly, but it's not about the answer, it's about demonstrating your 
creative problem solving skills. (it was tough, but I came up with 12!)
Best of luck!

Christopher Sheeder, HT(ASCP)QIHC
Pathology Manager | Department of Laboratories
Seattle Children's Hospital

-Original Message-
From: Blanca Lopez 
Sent: Thursday, September 12, 2019 11:48 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Help to interview new employees for the first time

I am going to interview people for Histotech position for the first time...what 
are the best questions to ask? How do I prepare myself? what is the I need to 
know that they are the best one? What should I ask or choose? Is good to put 
them in action like cutting or staining to check on their skills or what are 
your recommendations? thank you for your help

Blanca Lopez HT (ASCP)cm
Senior Histotechnologist
UT Southwestern Medical Center
Harold C. Simmons Comprehensive Cancer Center UTSTR Biorepository Tissue Lab
6000 Harry Hines Blvd NB5.102
Dallas, Texas 75390
214-648-7598
blanca.lo...@utsouthwestern.edu





UT Southwestern


Medical Center



The future of medicine, today.


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Re: [Histonet] tissue blocks stored in cooler C for 2wks - any successwith IHC?

[Sheeder, Christopher via Histonet]

Totally agree with Brett & Colleen.
It is very tempting to have an abundant source of study tissue but the results 
won't be worth the investment.
Best of luck.
Chris


Christopher Sheeder, HT(ASCP)QIHC
Histology Supervisor | Department of Laboratories
Seattle Children’s Hospital
4800 Sand Point Way NE
Seattle, WA 98105

Office: 206-987-6259

christopher.shee...@seattlechildrens.org


-Original Message-
From: Colleen Forster 
Sent: Thursday, August 22, 2019 11:27 AM
To: Brett Connolly 
Cc: M.O. ; Histonet 
Subject: Re: [Histonet] tissue blocks stored in cooler C for 2wks - any 
successwith IHC?

I agree with Brett.

I believe the histological aspect will be horrible and probably not worth the 
time and money spent. I feel you would be terribly disappointed with the lack 
of usable results.

Colleen Forster HT(ASCP)QIHC

On Thu, Aug 22, 2019 at 11:14 AM Brett Connolly via Histonet < 
histonet@lists.utsouthwestern.edu> wrote:

> Merissa,
> I have done tons of IHC assays on frozen tissues, including many
> procured from commercial tissue banks. My experience has been that the
> most of the time the microscopic morphology is terrible (abysmal if it
> is brain tissue). Cells are always blown open from slow freezing. It’s
> bad enough that there is a 2 week PMI…add to that storage at 4C
> instead of at least -20C (best would be -80C) and IMO it’s a recipe
> for failure. Wouldn’t touch that tissue no way, no how…well maybe if
> they paid ME I would take it and try to use it for autoradiography or 
> something.
> Just my opinion.
>
> Brett Connolly HTL (ASCP), PhD
>
> Sent from Mail for Windows 10
>
> From: M.O. via Histonet
> Sent: Thursday, August 22, 2019 11:24 AM
> To: Histonet
> Subject: [Histonet] tissue blocks stored in cooler C for 2wks - any
> successwith IHC?
>
> Good morning!
>
> We are working with a tissue bank that is only able to procure tissue
> blocks from donors that have postmortem intervals of 2wks or more. The
> storage conditions are in a cooler (I believe 4C), the block will be
> procured and frozen and we will harvest the tissue from that block
> once we thaw it. We plan to use these samples for histological purposes only.
>
> Has anyone had success with IHC on donors/tissues that were stored in
> 4C for 2wks or more? Will the integrity of the cells and proteins be okay?
>
> Thank you,
> Merissa
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>


--
Colleen Forster HT(ASCP)QIHC
BLS Histology and IHC Laboratory
B173 PWB  612-626-1930

*If submitting histology request please also forward to Lori Holm at 
ho...@umn.edu *

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Re: [Histonet] HSV-2 antibody - Cell Marque was not the problem

[Sheeder, Christopher via Histonet]

Beth,
You have honor and integrity. Something seldom seen these days.

Chris Sheeder
Seattle Children's Hospital

-Original Message-
From: O'Neil, Beth 
Sent: Wednesday, July 03, 2019 11:20 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] HSV-2 antibody - Cell Marque was not the problem

Last week I posted an inquiry about having problems with Cell Marque's HSV-2 
polyclonal antibody.  I have to retract my statement that it was Cell Marque 
using a different antibody source.   After spending two weeks troubleshooting 
my stainer, troubleshooting the positive control slides, yelling at my Cell 
Marque rep, etc.  I found out that it was the positive control slide and not 
the antibody.  When I originally suspected the positive QC slides (from Cancer 
Diagnostics), I requested a different lot number.  This new lot also failed to 
show positive staining.  Long story short, another call to Cancer Diagnostics 
finally resulted in receiving confirmation that they are having problems with 
their HSV-2 control slides.  It was my misfortune to have opened a new box of 
QC slides at the same time as opening a new vial of HSV-2 antibody which 
resulted in two weeks of headaches.  I will say that Cell Marque/Millipore was 
very supportive through this.  So, for those of you who are using Cancer 
Diagnostics HSV-2 control slides, they are working on a resolution and will 
replace their "bad" slides.  I was told that the staining is "hit or miss, "  
hopefully you all have the "hit" slides.

Beth Oneil, WVU Medicine




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