[Histonet] Re: Gas in Biohazard hood - safety issue

2010-03-14 Thread Stephen Kum Jew
One of our research labs has had tissue culture contamination problems.
One solution is to install a gas connection to flame the glass pipettes to
improve sterility.

Apart from the usual problems of upsetting the air flow within the
biohazard cabinet, I was wondering if the unburnt gas will build up to a
dangerous level within a closed recirculating air situation.

I have not found any incidents on the web about gas explosions in hoods.

I am waiting a reply from the manufacturer.

Has anyone had experience on this?

Thanks
Stephen


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[Histonet] Re: paraffin processing times for brain

2010-03-03 Thread Stephen Kum Jew
Hi all

I was wondering if anyone can send me please the optimal paraffin
processing times for brain autopsy tissue.

Currently the blocks have cut and stained well but 4-5mm thick tissue
has expanded (bulged out) following cutting as if they had absorbed water.

Solutions been all changed.

thanks
Stephen


|Stephen Kum Jew
|Senior Technical Officer
|Discipline of Pathology
|School of Medical Sciences
|Blackburn Building D06
|University of Sydney NSW 2006
|Australia
|Ph: + 61 2 9036 9027
|Fax:+61 2 9351 3429


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