[Histonet] Re: Gas in Biohazard hood - safety issue
One of our research labs has had tissue culture contamination problems. One solution is to install a gas connection to flame the glass pipettes to improve sterility. Apart from the usual problems of upsetting the air flow within the biohazard cabinet, I was wondering if the unburnt gas will build up to a dangerous level within a closed recirculating air situation. I have not found any incidents on the web about gas explosions in hoods. I am waiting a reply from the manufacturer. Has anyone had experience on this? Thanks Stephen ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: paraffin processing times for brain
Hi all I was wondering if anyone can send me please the optimal paraffin processing times for brain autopsy tissue. Currently the blocks have cut and stained well but 4-5mm thick tissue has expanded (bulged out) following cutting as if they had absorbed water. Solutions been all changed. thanks Stephen |Stephen Kum Jew |Senior Technical Officer |Discipline of Pathology |School of Medical Sciences |Blackburn Building D06 |University of Sydney NSW 2006 |Australia |Ph: + 61 2 9036 9027 |Fax:+61 2 9351 3429 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet