[Histonet] Decon disinfectant for Ultras
Has anyone used a disinfectant other than Lysol IC to do the decon on the Ventana Ultras? Thanks Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shun...@beaumont.edumailto:shun...@beaumont.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Discontinuing Negative Reagent Controls for IHC
Beaumont Hospital, Royal Oak MI. It's great! Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shun...@beaumont.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Monday, December 09, 2013 4:59 PM To: Roger Heyna; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Discontinuing Negative Reagent Controls for IHC Hartford Hospital, Hartford, CT Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax richard.car...@hhchealth.org From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Roger Heyna [rhe...@lumc.edu] Sent: Monday, December 09, 2013 3:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Discontinuing Negative Reagent Controls for IHC I am putting together a list of facilities that have discontinued the use of negative reagent controls for IHC. After the CAP revised the requirement related to negative controls when polymer-based detection systems are used, we decided to investigate whether discontinuing the negative controls would be possible for our lab. It would be helpful to know what labs have done this successfully. If the labs that have discontinued the use of negatives could just respond in an email, I would appreciate it. Also, if anyone has any thoughts pertaining to this change, they're certainly welcome. Thank you, Roger Maywood, IL This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: CAP survey Question
We do the same process as Linda does here at Beaumont. Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shun...@beaumont.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Wednesday, December 04, 2013 4:34 PM To: 'Vickroy, Jim'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: CAP survey Question We also have Ultras Jim. We don't print out the run logs. IHC personnel review the controls before they go to a pathologist in order to catch any problems. The pathologists are supposed to review the controls associated with each case they sign out. Our pathology report has a statement included that the negative and positive controls have stained appropriately. By signing off on each case, the pathologist is attesting to the fact that he has indeed reviewed the controls...we do not police them. So far, this works for us. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Wednesday, December 04, 2013 2:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP survey Question For a long time I have had our IHC techs print out the run logs from each IHC run on the Benchmark Ultras. The techs then check the slides to make sure the positive and negative controls have worked properly before the slides are sent to the individual pathologists. The pathologists are also supposed to check the controls before looking at the patient slides. Lately in the interest of reducing turnaround time I have been asked why we run the log reports and have a tech look over the controls before they send them to the pathologists since the pathologist will also evaluate the controls. I have been doing this because I wanted the documentation that someone reviewed the controls each time an IHC stain was done. I believe if the pathologists would document someplace that the control slides were reviewed before the patient slides were viewed then I could eliminate the techs looking over the controls also. Problem is how are others documenting that the controls are reviewed? Is this done by the techs, the pathologists, or both? We of course have also used this data for quality assurance of our stains. Thanks for your help. Jim James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] M2D2 and SMAD
Good morning Does anyone have suggestions for antibodies for M2D2 and SMAD? We have not been able to get these antibodies to work for us on the Ventana Ultras. If you do have suggestions, protocols and control tissue suggestions would also be appreciated. Thanks Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shun...@beaumont.edumailto:shun...@beaumont.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: maintenance agreement for autostainer
Try Victor Wong, Autostainer Technical Solutions 888-505-9545 cell 646-378-9222 www.autostainertech.com Not sure if he is still in business, he was located in New Windsor NY Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shun...@beaumont.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Zerfas, Patricia (NIH/OD/ORS) [E] Sent: Thursday, October 17, 2013 1:07 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] maintenance agreement for autostainer Do you or do you know someone that offers a service maintenance agreement for a 10+ year old DAKO autostainer? Thanks, Patricia M. Zerfas Building 28A, Room 112, MSC 5230 9000 Rockville Pike Bethesda, MD USA 301-496-4464 Fax 301-402-1068 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: manual IHC staining
Wow - I can't imagine wanting to go to manual staining for 20,000 slides per year. That's about 80-100 per day. We used to do manual staining but only about 20-40 slides per day. Unless you are running a very limited panel of antibodies, you will be hopping to keep up with all the steps. I wouldn't think you would be batching all slides in one run, so you will have timers going off all the time. You or your techs will not have time to do anything else but stain, wash, apply reagents, wash, etc. You might want to do some trial runs for timing, work flows etc before you make the decision to ditch the autostainer. I would also consider consistency of staining , correct antibodies being added to the correct slides, and amount of reagents (no matter how careful you are, you will put more on than the stainer). Just my thoughts. We do 30-35,000 slides per year, we have three Ventana Ultras and they are busy all the time. and so are my 2 techs. Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shun...@beaumont.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Histology Sent: Wednesday, October 16, 2013 12:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] manual IHC staining Hi all- Does anyone out there still do manual, offline IHC staining? We currently do about 20,000 IHC slides per year and my boss would like to consider dropping the automatic stainers and doing them by hand. If you do manual staining, how long does it take, how much tech time is needed, and what about CAP inspections? Thanks in advance, Mehndi Helgren Dominion Pathology Laboratories 733 Boush St. Suite 200 Norfolk, VA 23510 757-664-7901 hi...@pathlab.us ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Peggy Wenks award
For those of you who may not be attending the National Convention or did not hear, we at Beaumont are so proud to announce that Peggy Amazing Wenk has won the J.B. McCormick Award. This very prestigious award was presented by Dr. McCormick and was given to Peggy for her outstanding and exceptional service to the NSH. Please join us in congratulating Peggy on this well deserved recognition and award. Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shun...@beaumont.edumailto:shun...@beaumont.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] FISH enumeration
My techs do the scoring for the FISH testing. We have a Leica Ariol system - the HER2 FISH are computer assisted, but the Urovysions are manually assessed. That may change with the new systems we are receiving. My techs are exceptionally well trained in morphology - they sit with our director to learn, and are really really good. We do about 10 urovysions per week and sometimes as many as 30 pathvysions per week. I have one or two techs on the FISH rotation each week. The other techs step in as needed. The pathologist circles the area on the HER2 IHC slide to be FISHed. The Pathologists do come down to the lab to see cases, but they rely on the techs. The pathologists also look at all other criteria before signing out the case to make sure everything fits. We are also getting a new image hub so the saved images will be viewable by the pathologists - this will be a really nice addition to the reviewing process. I believe there are many laboratories where the cytotechs read out the urovsyions. Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shun...@beaumont.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Saturday, September 07, 2013 10:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FISH enumeration For any laboratories out there who perform in house FISH procedures, if you could share what personnel are responsible for doing the signal enumeration scoring? It would be helpful if you could describe the personnel's training and certification, as well as an approximation of FTE's needed with some volumes. Do you do manual enumeration or use scanning software? Thanks for any input. Joelle Weaver MAOM, HTL (ASCP) QIHC From: tmcne...@lmhealth.org To: thisis...@aol.com; histonet@lists.utsouthwestern.edu Date: Fri, 6 Sep 2013 05:45:41 -0400 Subject: RE: [Histonet] Cell Block Preparation CC: This is how we do it now. In the old days, we used agar and to my mind, it is still the best way when you have scant material. - Spin in a conical tube and pour off - Melt an agar slant (we get TSA slant from micro) - Pour the agar into the conical tube and spin for 5 minutes - The agar will re-solidify and whatever sediment there is will be concentrated in the very tip of the cone - The agar will slide out of the centrifuge tube - Slice off the very tip and wrap in lens paper - Place the wrapped tip in a cassette and process as usual - Embed the specimen tip down and you are good to go... I still use this method today when I feel it necessary. Works great. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ann Specian Sent: Thursday, September 05, 2013 12:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cell Block Preparation I am getting complaints in regard to insufficient cell blocks. We currently spin, pour off the supernatant, retrieve the sediment and process in lens paper. Does anyone have a more current technique which renders better cellularity? Also, do you know which renders a better cell block: a fresh specimen, a specimen fixed in Cytolyt or a specimen fixed in 10% NBF? Thanks, Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet
RE: [Histonet] Losing tissue on IHC slides
Good Morning Another thing to try is different slides. We have had issues with bad batches/lots of slides - even the Fisher Superfrost plus. Especially if your purchasing department buys in quantity and then stores the slides in hot warehouses. This seems to negate the charge on the slides - but in an inconsistent way. You can have bad slides mixed with good ones in the same box. Even if you don't want to change vendors, trying a different kind of charged slide would eliminate bad slides as part of the problem. And just an FYI- we do our control slides the same way you do, (and use tap water) so I don't think that is your problem. We are using the cheaper Fisher Superfrost plus slides and they seem to work as well as the full priced ones. We cut our immuno sections at 3um. I know how frustrating this can be - I feel your pain! Good luck! Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shun...@beaumont.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Deloris Carter Sent: Friday, July 26, 2013 5:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Losing tissue on IHC slides Hi all, I'm looking for some troubleshooting help. In the last couple of months we've been having an increasing trend in losing tissue from our IHC slides. We use a Ventana Benchmark XT. PM is done on it quarterly. The problem seems to be with the patient tissue, not the control tissue. We use Fisherbrand Superfrost Plus slides, picking up the control by dipping the top end of the slide to avoid double dipping. We use tap water in the waterbaths which I know could cause an issue, but that's the way it's always been done here, so there's been no change to that part of the procedure. (We did try using DI water, but it didn't make any difference. Tissue still fell off of slides.) We do use DI water to mix the bulk reagents.The control slides may sometimes have been cut for a longer than recommended time, but that part of the procedure hasn't changed either, same process as always. If we run separate slides for control and patient tissue, we get much better results, but still have some tissue loss. I can't say it's all of our tests, but it is a steadily worsening problem. I'm going through tons of reagent, and I can't seem to nail down the problem. Ventana rep said to try DI water, don't double dip, try adhesive slides (which didn't really make any difference that we could tell). I would appreciate any suggestions Deloris Carter Shawnee Mission Medical Center ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Her-2 antibody
We have a Bond Max and run mainly ER/PR and ISH stains on that. After Leica loaded in a new version of software in October we started having problems like that too. Seems you have to have all 10 spots on the tray filled with a slide - doesn't have to be programed, just a slide and cover tile for the weight. We also were doing a workaround with a Vantage label under the Leica label and found out that the two label thickness interferes with the movement of the cover tile (sometimes...but not always), which was affecting our staining. Even a regular slide label under the Leica label will cause a problem. We finally have gone to entering a case, printing the labels but not putting them on the slides, cutting the sections and then putting the labels on the slide for positive patient identification. Not ideal, and I worry a lot about case mix ups, but that is what is working to get consistent staining here. As they say, The Enemy of Good is Better. Wish Leica had not made it better! It was working very nicely before. Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shun...@beaumont.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robert Fauck [CCDHB] Sent: Tuesday, June 11, 2013 1:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Her-2 antibody Hi All We here in wellington Hospital New Zealand having some problem with the Her-2 ( Lieca, NCL-L-CBE-356 ) Top half of the slide ( control or Test tissue) are strongly positive but if we put the control tissue on the bottom half it is week to negative! Does any one has the same problems with your Her-2, it is done on a Leica Bond (Max) III. Much appreciated for any suggestions, also from Leica Technical specialists. Regards, Robert Fauck Wellington hospital, NZ This email or attachment(s) may contain confidential or legally privileged information intended for the sole use of the addressee(s). Any use, redistribution, disclosure, or reproduction of this message, except as intended, is prohibited. If you received this email in error, please notify the sender and remove all copies of the message, including any attachments. Any views or opinions expressed in this email (unless otherwise stated) may not represent those of Capital Coast District Health Board. http://www.ccdhb.org.nz (1C_S1) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Her-2 antibody
We have contacted Leica several times, had the machine calibrated/aligned many times, and that is what we have been told. It seems to hold true as with only one label we have no problems. Also, after the upgrade, we saw the problem a lot when we only ran one or two slides on a tray. The magic number seems to be 4 slides to get acceptable staining, but Leica suggests we load it up. We had never had a problem until the upgrade in software. Sue -Original Message- From: Houston, Ronald [mailto:ronald.hous...@nationwidechildrens.org] Sent: Wednesday, June 12, 2013 10:59 AM To: Sue Hunter; Robert Fauck [CCDHB]; histonet@lists.utsouthwestern.edu Subject: RE: Her-2 antibody Never had any such problems with either the Max or the Bond IIIs in the 6+ years we have had them(no matter how many labels are on the slide - we have had three labels on a slide with no problems at all), unless of course your tissue extends to the rim of the covertile - then what would you expect? You certainly do not need to have all 10 spaces filled on the tray. Suggest you contact Leica service as it seems as though your machine needs calibrated and realigned Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.hous...@nationwidechildrens.org www.NationwideChildrens.org One person with passion is better than forty people merely interested. ~ E.M. Forster -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue Hunter Sent: Wednesday, June 12, 2013 10:42 AM To: Robert Fauck [CCDHB]; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Her-2 antibody We have a Bond Max and run mainly ER/PR and ISH stains on that. After Leica loaded in a new version of software in October we started having problems like that too. Seems you have to have all 10 spots on the tray filled with a slide - doesn't have to be programed, just a slide and cover tile for the weight. We also were doing a workaround with a Vantage label under the Leica label and found out that the two label thickness interferes with the movement of the cover tile (sometimes...but not always), which was affecting our staining. Even a regular slide label under the Leica label will cause a problem. We finally have gone to entering a case, printing the labels but not putting them on the slides, cutting the sections and then putting the labels on the slide for positive patient identification. Not ideal, and I worry a lot about case mix ups, but that is what is working to get consistent staining here. As they say, The Enemy of Good is Better. Wish Leica had not made it better! It was working very nicely before. Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shun...@beaumont.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robert Fauck [CCDHB] Sent: Tuesday, June 11, 2013 1:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Her-2 antibody Hi All We here in wellington Hospital New Zealand having some problem with the Her-2 ( Lieca, NCL-L-CBE-356 ) Top half of the slide ( control or Test tissue) are strongly positive but if we put the control tissue on the bottom half it is week to negative! Does any one has the same problems with your Her-2, it is done on a Leica Bond (Max) III. Much appreciated for any suggestions, also from Leica Technical specialists. Regards, Robert Fauck Wellington hospital, NZ This email or attachment(s) may contain confidential or legally privileged information intended for the sole use of the addressee(s). Any use, redistribution, disclosure, or reproduction of this message, except as intended, is prohibited. If you received this email in error, please notify the sender and remove all copies of the message, including any attachments. Any views or opinions expressed in this email (unless otherwise stated) may not represent those of Capital Coast District Health Board. http://www.ccdhb.org.nz (1C_S1) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra
We run our DIF on our old Lab Vision stainer because it is easier and cheaper. The Ultra ready to use Abs are very expensive and there is no negative control. As far as we can find out, there is no prep kit that you can use with the fluorescence protocols to make your own. Ventana's solution is to put your negative slide in a coplin jar filled with buffer and then add that to your slide tray when you are done. We have the same issue with our Bond Max stainer - you have to use a detection kit for everything on the Bond so Leica's solution is to sell you a kit really cheap where you only use one solution and throw the rest away. Very expensive. Since neither one of these solutions is an acceptable one for us, we still do them the oldway and are very happy. I would think that unless you are running a really large number of cases, even doing them by hand is preferable to running them on the Ultra. Just my opinion. Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shun...@beaumont.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mel John del Barrio Sent: Monday, April 22, 2013 12:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra Hi All Anyone in the group utilises the Benchmark for DIF's? What sort of controls do you use to validate the assay ? What problems you have encountered? Thanks MJ Image by FlamingText.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Out of my comfort zone...
Sarah The proteinase K does a lot more than break the formalin linkages. To isolate the RNA or DNA you have to break up the cell membranes, nuclear membranes and all the other proteins in the cellular matrix to isolate the nucleic acids. I don't think an antigen retrieval solution will do any of that. We use a kit from Qiagen that is very easy to use. Also, if you haven't done much RNA work, remember that there are RNAses everwhere. Wear gloves, wipe down your microtome with RNAse away or some other such product. Use a clean blade. Discard the first couple of cuts from your block. Too many fingers have touched them. We usually cut 10um sections for our extractions. Use clean water in your waterbath - fresh just for your RNA tissues. We keep slide boxes separate for RNA work so bare fingers don't touch them. As for how many sections - it depends on how much message you will be looking for. You will have to try your method to find out.If you cut curls for extractions, we use 10um curls. Use disposable plastic tubes as these are mostly RNAse free. We routinely isolate sufficient quantities of good RNA from FFPE tissues, but you still need to use good RNA technique. If you are making up your own master mixes and primer mixes, be sure to use RNAse free water. Good luck Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shun...@beaumont.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sarah Dysart Sent: Tuesday, April 02, 2013 5:36 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Out of my comfort zone... So...I have been asked to do some micro-dissection on some slides and then do downstream RT/PCR on them. My molecular knowledge doesn't go much out of the world of IHC so...here is my question... Has anyone ever substituted Citrate Buffer pH6 (or whatever HIER solution you are using) for proteinase K for use in RNA isolation and then later PCR? Does this work? The main question is will the HIER step take off the formalin linkage from the nucleic acids, or just the protein? One last thing is what else goes into these solutions other than Citrate Buffer and Tween? I haven't made it up in forever, I have just been ordering it from companies...I know...lazy... Thanks Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Out of my comfort zone...
Hello Just wanted to add one more thing - we actually use a dedicated pyrex dish (maybe 6x10 inches) for our water bath for RNA sections. We use warm tap water, but you can put it in the microwave for a short bit if it needs to be warmer. You can spray the dish with RNAse away and wipe before filling with water. Sue -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sarah Dysart Sent: Tuesday, April 02, 2013 5:36 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Out of my comfort zone... So...I have been asked to do some micro-dissection on some slides and then do downstream RT/PCR on them. My molecular knowledge doesn't go much out of the world of IHC so...here is my question... Has anyone ever substituted Citrate Buffer pH6 (or whatever HIER solution you are using) for proteinase K for use in RNA isolation and then later PCR? Does this work? The main question is will the HIER step take off the formalin linkage from the nucleic acids, or just the protein? One last thing is what else goes into these solutions other than Citrate Buffer and Tween? I haven't made it up in forever, I have just been ordering it from companies...I know...lazy... Thanks Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Immunofluorescence on FFPE skin
Hi Erin We do direct immunofluorescence staining on FFPE kidney biopsies but instead of microwave antigen retrieval, we use proteinase K (20mg/ml Qiagen Cat # 19131) diluted 1/10 in Tris buffered saline for 20 minutes at Room Temp. We also incubate with the antibody (1/5 dilution) for 90 minutes. Don't remember if our student tried using the microwave (it was a student project) but I would certainly give it a try. There really is no difference with the wattage for a microwave - the trick is to get the solution boiling - THEN start your timing for retrieval. We do our AR for our FISH testing for 20 minutes at half power after it comes to a boil. This allows the solution to keep at near boiling temp but not to over flow the container when it comes back to a boil. The timing for using a pressure cooker would have to be validated - now you are adding an additional parameter of pressure to factor in to bring the solution to a boil. Good luck - hope it works for you. Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shun...@beaumont.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martin, Erin Sent: Monday, March 18, 2013 2:28 PM To: histonet Subject: [Histonet] Immunofluorescence on FFPE skin Hello all, My pathologist gave me a copy of Immunofluorescence with Dual Microwave Retrieval of Paraffin-Embedded Sections in the Assessment of Human Renal Biopsy Specimens from the American Journal of Clinical Pathology. He said that the same principle should work on skin and he would like to be able to do IF on fixed tissue in addition to our usual cryostat sections. Has anyone else read the paper who might be willing to give me some basic advice to try working it out? Is a microwave necessary (paper's method uses 2 different wattage settings) or is there a way to use HIER in waterbath or pressure cooker? Thanks in advance, Erin Erin Martin, Histology Supervisor UCSF Dermatopathology Service 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or priviledged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: SOS EGFRV3
Billie The last I heard (several years ago), this antibody was originally produced at a university - Duke maybe? They considered marketing it but then decided not to. I do not know of any other antibody for this mutation. We have a RT-PCR assay for it, but are not using it as there was no interest by our clinicians. If you ever find an antibody, please let me know. I would be interested in trying it also. Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shun...@beaumont.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Zimmerman, Billie Sent: Thursday, March 14, 2013 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] SOS EGFRV3 Does anyone perform this particular clone of EGFR?? Thanks in advance for your help. Billie Zimmerman MT(ASCP)QIHC 706-721-5617/3630 Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to bzimm...@gru.edu. Please update your address book to reflect this change. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: SOS EGFRV3
Thanks for the update. sue From: Jim Burchette [mailto:jburc...@gmail.com] Sent: Friday, March 15, 2013 11:02 AM To: Sue Hunter Cc: Zimmerman, Billie; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: SOS EGFRV3 Sue, you are correct, EGFR v III was developed at Duke by Dr Darell Bigner. BA, you can reach him at 929-684-5018. Email is big...@duke.edumailto:big...@duke.edu I ran this antibody when I was there. Email me for IHC specifics. Best, JB On Mar 15, 2013 9:25 AM, Sue Hunter shun...@beaumont.edumailto:shun...@beaumont.edu wrote: Billie The last I heard (several years ago), this antibody was originally produced at a university - Duke maybe? They considered marketing it but then decided not to. I do not know of any other antibody for this mutation. We have a RT-PCR assay for it, but are not using it as there was no interest by our clinicians. If you ever find an antibody, please let me know. I would be interested in trying it also. Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146tel:248-898-5146 shun...@beaumont.edumailto:shun...@beaumont.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edumailto:histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edumailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Zimmerman, Billie Sent: Thursday, March 14, 2013 2:39 PM To: histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu Subject: [Histonet] SOS EGFRV3 Does anyone perform this particular clone of EGFR?? Thanks in advance for your help. Billie Zimmerman MT(ASCP)QIHC 706-721-5617/3630 Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to bzimm...@gru.edumailto:bzimm...@gru.edu. Please update your address book to reflect this change. ___ Histonet mailing list Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: CD68
We use Ventana's ready to use for the Ultra, but it is clone KP1. You can get the same clone in a concentrate from Cell Marque. Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shun...@beaumont.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Wednesday, March 13, 2013 9:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD68 Good Morning! CD68 (for the BOND)is backordered until May and I am looking for recommendations - Cellmarque or Biocare? Is there something better? I appreciate your input. Nancy Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Antibody IDH1
We use Dianova's antibody at a 1:100 dilution on the Ultra. CC1 cell conditioning for 40 minutes, antibody for 32 minutes - no heat. We use Optiview detection system. No amp. Make sure you are using the correct type of brain tissue for a control - most oligodendrogliomas should be positive, altho there are a few with other mutations. Remember that this antibody only picks up the R132H point mutation. This occurs in about 70% of oligos so it is possible that you happened to pick one that was positive for one of the other mutations. Perhaps try a few different patient tissues. Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shun...@beaumont.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Zimmerman, Billie Sent: Friday, February 22, 2013 4:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Antibody IDH1 Does anyone perform this antibody?? We can't get it to work on the Ventana Ultra. PhenoPath in Seattle performs it on the Dako autostainer using a small size polymer detection kit. I would appreciate any input. Thanks, Billie Zimmerman Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to bzimm...@gru.edu. Please update your address book to reflect this change. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] IgG4
We use Cell Marques Mouse monoclonal (clone MRQ-44) Cat# 367M-16 at a 1:200 dilution on the Ultra. We are using the Optiview detection kits. CC#1-24 min Option 5 - 12 min Antibody - 24 min OV Linker 8 min Multimer 8 min H2O2 +DAB 8 min OV copper 4 min Hematozylin II 8min bluing 4 min We only test FFPE tissues. Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shun...@beaumont.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Susan Foreman Sent: Monday, February 25, 2013 2:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IgG4 Has anyone worked up IgG4 on the Ventana Benchmark Ultra? What vendor? How about running it on fresh frozen sections? FITC? Vendor? Many Thanks for your input, Susan ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Enquiry about blood removal from frozen tissue sections
You could try hydrogen peroxide - it wil get rid of the heme in the RBCs which is probably giving you the background. I would try a 1:10 dilution (or higher) of the H2O2 (in PBS) that you usually get from the store/pharmacy - undiluted will probably bubble too much and lift off the tissue. You will have to try dilutions and time - usually 10 minutes is good, but with a diluted solution you may need to go longer. Then wash the slides in PBS to get rid of the H2O2. Good Luck Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shun...@beaumont.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of girish cm Sent: Tuesday, December 11, 2012 12:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Enquiry about blood removal from frozen tissue sections Hello, I would like to know whether any methods (other than cold PBS wash) or products are available to remove the blood components from frozen tissue sections. My application is antibody targeted spectroscopic imaging in which the blood components are providing background noise. Thanking you, Regards -- Girish C M Senior Research Fellow Amrita Centre for Nanoscience Molecular Medicine Amrita Institute of Medical Sciences Research Centre Cochin, Kerala India-682 041 Ph: 9645095045 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RNA isolation form stained slides
Have you tried to extract the RNA from the tissue without the stain- just to test your method? I am not familiar with the mechanics of the stain but you might be denaturing the RNA. Also are you sure there is enough message in the area you are trying to extract to get enough RNA? Maybe you need more material. Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shun...@beaumont.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vanessa Orsini Sent: Friday, November 02, 2012 5:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RNA isolation form stained slides Hello, I need to extract RNA for a RT-PCR after Laser MicroDissection on xGal stained slides. I tried using sections from unfixed frozen organs. I fixed the sections in EtOH70% for 10min and then I stained them with xGal for 3h at 37°C. After air drying I cut out with the LCM and extract RNA with the PicoPure kit from Applied Biosystem. So far I didn't manage to get enough RNA. I tried to add RNase inhibitors to all the solutions but it didn't help. Any idea/suggestion? Do someone think it would be better to do a LacZ antibody staining on FFPE sections and extract RNA with an appropriate kit? The RNase would they be less active? Thank in advance for any help you can give me J Vanessa ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Billing 88342
It is my understanding that you can only bill for one 88342 on a specimen - technical or professional, even if you perform the same stain on multiple blocks of that specimen. For A1, A2, A3 etc you can only charge once. For A1, B1, C1, etc you may charge a 88342 per specimen. Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shun...@beaumont.edu Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Victor A. Tobias Sent: Tuesday, July 03, 2012 12:43 PM To: 'HISTONET' Subject: [Histonet] Billing 88342 Looking for other opinions from those who do consult/referral work. If a client sends in a request for a single antibody done on multiple blocks on a single specimen, do you bill the client for each tech component ? The client will do the interpretation. What happens in the above scenario if the request is to bill the patient? Knowing you get reimbursed for one, do you eat the other charges are make the client select the one block? We have run numbers on potential lost revenue and the number is significant. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB244 Seattle, WA 98104 vtob...@u.washington.edumailto:vtob...@u.washington.edu 206-744-2735 206-744-8240 Fax =Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet To report this email as SPAM, please forward it to s...@websense.com. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Fluorescence-filters
We recently installed a LED light source on our fluorescence scope that our pathologists use for reading IF on kidneys and skins. They love it. No more having to warm up the bulb, not turning it off too soon, or my nightmare - leaving it on overnight. The bulb is supposed to last a really long time and gives off a nice bright light. Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shun...@beaumont.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Tuesday, June 05, 2012 7:32 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Fluorescence-filters Can you do fluorescence with LED? (That may be a stupid question, as I always thought LED was just for brightfield). Emily You see a peanut, day's off to a good start; you witness some soil it's a jamboree for Vince Noir. --Howard Moon, in Charlie, The Mighty Boosh ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet To report this email as SPAM, please forward it to s...@websense.com. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HistoBath, HistoChill, Clini-RF
Can you use the 3M freezing fluid in a histobath instead of isopentane? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of abri...@brightinstruments.com Sent: Tuesday, April 10, 2012 6:46 AM To: Bob Richmond; histonet-boun...@lists.utsouthwestern.edu; Histonet Subject: Re: [Histonet] HistoBath, HistoChill, Clini-RF Dear Bob, I would just like to point out that the recommended freezing fluid for the Bright Clini-RF Rapid -80c tissue freezer is 3M's Novec HFE-7100 not 7000 as you state. Best regards Alan Bright www.brightinstruments.com Sent from my BlackBerry(r) wireless device -Original Message- From: Bob Richmond rsrichm...@gmail.com Sender: histonet-boun...@lists.utsouthwestern.edu Date: Mon, 9 Apr 2012 09:16:23 To: Histonet@lists.utsouthwestern.eduhistonet@lists.utsouthwestern.edu Subject: [Histonet] HistoBath, HistoChill, Clini-RF Terri Bishop at SPScientific sent me an e-mail about HistoChill, a frozen section freezing bath that replaces the discontinued HistoBath. Terri didn't feel it was appropriate for a vendor to post this directly on HistoNet, so I am. You can contact Terri Bishop at terri.bis...@spscientific.com HistoChill has been available for about a year. You can see the brochure at http://www.spscientific.com/Air-Stream-/-Baths-/-Chillers-/-Traps-/-Probes.aspx I'm pleased that they are specifically recommending using 3M's non-flammable perfluorocarbon HFE-7000 coolant, and not isopentane or acetone. (I feel like I've struck a blow for lab safety!) As has been noted on HistoNet before, Hacker Instruments offers Alan Bright's Clini-RF, a competing product. I have no commercial connection with any of the companies I've mentioned, and I have no personal experience with either instrument. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- BEGIN-ANTISPAM-VOTING-LINKS -- Teach SpamSniper if this mail (ID 01GTNjypu) is spam: Spam: http://admin.spamsniper.co.uk/canit/b.php?i=01GTNjypum=00c557cef5d5t=20120409c=s Not spam: http://admin.spamsniper.co.uk/canit/b.php?i=01GTNjypum=00c557cef5d5t=20120409c=n Forget vote: http://admin.spamsniper.co.uk/canit/b.php?i=01GTNjypum=00c557cef5d5t=20120409c=f -- END-ANTISPAM-VOTING-LINKS This message has been scanned and no issues discovered. To report this email as SPAM, please forward it to s...@websense.com. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Leica Bond IHC Platform
We have a Bond Max in my lab and love it! It has been very dependable and easy to use . We also have three Ventana Ultras and two Lab Visions. We originally got the Bond for EBV and Kappa/Lambda in-situ but also do our hormone receptors and a few other immunos on it. Our pathologists felt the signal obtained with the DAB on the in-situ slides was superior to the NBT of the Ventana slides. The one draw back that Ventana will talk about is that you have three racks of ten slides - each rack is independent of the others, but not the continual load of the Ultras. You also cannot mix pretreatments on each rack because of timing/heating issues. But we have not found either of these two issues to be a problem for us. The Bond III is supposed to be even faster than the Bond Max but haven't looked at that. Sue Sue Hunter Supervisor, Advanced Diagnostics Beaumont Health Systems Royal Oak MI 48073 248-898-5146 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Wellen, Terrence D. :LPH Lab Sent: Wednesday, April 04, 2012 8:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica Bond IHC Platform Does anyone have any experience with this product? Terrence Wellen HT(ASCP) Legacy Good Samaritan Hospital Portland, OR ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message has been scanned and no issues discovered. To report this email as SPAM, please forward it to s...@websense.com. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Leica Bond IHC Platform
I've only been told that the Bond III could do a run in, say 3 hours, where the Bond Max might be done in 4.(don't take these times as true - just as an example). We love the ability to do a delayed start so the slides are done when we come in the next morning - especially the in-situ slides that take longer than the immunos. sue -Original Message- From: Rathborne, Toni [mailto:trathbo...@somerset-healthcare.com] Sent: Thursday, April 05, 2012 10:51 AM To: Sue Hunter; Wellen, Terrence D. :LPH Lab; histonet@lists.utsouthwestern.edu Subject: RE: Leica Bond IHC Platform We have the Bond III. No complaints. I'm not sure what to compare to regarding speed, but we can do 4 runs a day if necessary. This does not include an overnight run, which we do as needed. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue Hunter Sent: Thursday, April 05, 2012 9:24 AM To: Wellen, Terrence D. :LPH Lab; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Leica Bond IHC Platform We have a Bond Max in my lab and love it! It has been very dependable and easy to use . We also have three Ventana Ultras and two Lab Visions. We originally got the Bond for EBV and Kappa/Lambda in-situ but also do our hormone receptors and a few other immunos on it. Our pathologists felt the signal obtained with the DAB on the in-situ slides was superior to the NBT of the Ventana slides. The one draw back that Ventana will talk about is that you have three racks of ten slides - each rack is independent of the others, but not the continual load of the Ultras. You also cannot mix pretreatments on each rack because of timing/heating issues. But we have not found either of these two issues to be a problem for us. The Bond III is supposed to be even faster than the Bond Max but haven't looked at that. Sue Sue Hunter Supervisor, Advanced Diagnostics Beaumont Health Systems Royal Oak MI 48073 248-898-5146 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Wellen, Terrence D. :LPH Lab Sent: Wednesday, April 04, 2012 8:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica Bond IHC Platform Does anyone have any experience with this product? Terrence Wellen HT(ASCP) Legacy Good Samaritan Hospital Portland, OR ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message has been scanned and no issues discovered. To report this email as SPAM, please forward it to s...@websense.com. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. This message has been scanned and no issues discovered. To report this email as SPAM, please forward it to s...@websense.com. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
