[Histonet] Celestine Blue for Technovit 7200
Hi everyone, I'm searching for a procedure or help w/a stain for thick ground sections using Technovit 7200. The stain in question is a celestine blue/alum hematoxylin w/a Van Gieson counterstain. Anyone have a procedure or recommendations? Thanks in advance, Suzanne sbr...@vetpathservicesinc.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Celestine Blue for Technovit 7200
Thanks Jack, what's the difference between the celestine blue w/alum hematoxylin and the Sanderson's? What's the difference between the Sanderson's and the Masson's Goldner's? Have you tried the celestine blue stain? Thanks in advance, Suzanne From: Jack Ratliff [mailto:ratliffj...@hotmail.com] Sent: Wed 10/20/2010 5:04 PM To: Suzanne Bruce; Histonet Subject: RE: [Histonet] Celestine Blue for Technovit 7200 Why not use Sanderson's Rapid Bone Stain with Van Gieson picrofuchsin? You can get the Sanderson's and one or all of three counterstains for this particular kind of staining in a kit from Dorn and Hart Microedge (www.dornandhart.com http://www.dornandhart.com/ ). Jack Date: Wed, 20 Oct 2010 16:52:26 -0400 From: sbr...@vetpathservicesinc.com To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Celestine Blue for Technovit 7200 Hi everyone, I'm searching for a procedure or help w/a stain for thick ground sections using Technovit 7200. The stain in question is a celestine blue/alum hematoxylin w/a Van Gieson counterstain. Anyone have a procedure or recommendations? Thanks in advance, Suzanne sbr...@vetpathservicesinc.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Cell phones
The cell phone do not call list is real. In Ohio several people I know started getting lots of 'spam' calls on their cell phones. They called their carrier (verizon) and asked how to stop it. They recommended the government do not call list. Once they called that number the spamming stopped. If you read the snopes website the information stops in 2006. Call your cell phone carrier if you are concerned instead of checking the internet if you are receiving these annoying calls. Suzanne ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Autoradiography
Does any one know of a company that does Autoradiography (Tritium) on rabbit eyes? Thanks so much in advance, Suzanne ___ Suzanne Bruce, R.V.T. Histologist Necropsy Coordinator ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE:guide to special stains
http://www.dako.com/prod_productrelatedinformation?url=literature_guides.htm You can also email them and request the guide. Or you can download the PDF, but it is VERY long, too much to print out. Does anyone have or know where to obtain a list of special stains that briefly describes which each one is for? Thanks, Patricia Zerfas National Institutes of Health Building 28A, Room 112 28 Library Drive Bethesda, MD 20892 ph: (301) 496-4464 fax: (301) 402-1068 -- Does anyone have or know where to obtain a list of special stains that briefly describes which each one is for? Thanks, Patricia Zerfas National Institutes of Health Building 28A, Room 112 28 Library Drive Bethesda, MD 20892 ph: (301) 496-4464 fax: (301) 402-1068 -- Does anyone have or know where to obtain a list of special stains that briefly describes which each one is for? Thanks, Patricia Zerfas National Institutes of Health Building 28A, Room 112 28 Library Drive Bethesda, MD 20892 ph: (301) 496-4464 fax: (301) 402-1068 -- ___ Suzanne Bruce, R.V.T. Histologist Necropsy Coordinator Vet Path Services, Inc. (VPS) 6450 Castle Dr. Mason, OH 45040 Lab: (513) 469-0777 Fax: (513) 469-2474 Email: sbr...@vetpathservicesinc.com www.vetpathservicesinc.com http://www.vetpathservicesinc.com/ https://69.61.197.115/exchweb/bin/redir.asp?URL=http://www.vetpathservicesinc.com/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE:Sanderson's RBS image
Thanks Gayle, I also looked at the Surgipath image, my slide was much more brilliant then that one. I did use the acid fuchsin counter stain. I just wasn't sure if mine would be considered 'over stained.' I'll send you an image that Jack Ratliff sent me that compares to what I was seeing today. I'm not using GMA, but Technovit 7200 ~100 micron thick ground sections. Suzanne Date: Thu, 19 Mar 2009 10:38:51 -0600 From: gayle callis gayle.cal...@bresnan.net Subject: [Histonet] RE: Sandersons rapid bone stain photo To: 'Histonet' histonet@lists.utsouthwestern.edu Message-ID: 000401c9a8b1$30e43630$92aca2...@callis@bresnan.net Content-Type: text/plain; charset=us-ascii On looking at the Surgipath photo, the bone is very pale although the osteocytes, osteoid and soft tissues look good. I am not sure what counterstain (two are touted in the stain data sheet) was used as seen in this photo. One is a basic fuchsin that turns the bone red. Be careful of overstaining with fuchsin counterstain. The other is light or fast green and since we never used that, I can't comment on the color contrast with bone and soft tissue components. This may be the stain used in photograph??? It always is a good idea to look at the stain while performing the actual staining method. After prescribed or desired time in RBS, simply rinse quickly with running hot tap water, blot and examine under the microscope to see depth of stain for soft tissue/bone components. To view a dry section, simply place a coverglass on top of section for higher power examination. If components stained by RBS not dark enough, you can return to the hot RBS. Rinse then blot, and counterstain after that. It does not hurt the bone section to dry. In fact we preferred a dry section when going back into either stain just to prevent dilution of the stain solution, particularly when doing hand ground thick section surface staining. Gayle Callis HTL(ASCP)HT,MT Bozeman MT 59715 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Sanderson's Rapid Bone Stain
Here are my responses: Suzanne: I no longer use Sanderson's, however we did have nice results on plastic embedded sections, in conjuction with other calcified stains. Use caution in over-staining. I do not recall ever reading about usage on paraffin. The Villanueva Osteochrome bone stain can also be used on calcified - we used it with fluoro labels, and then viewed using confocal microscopy with beautiful architecture and active new bone formation. Vicki K Sanderson's - we've not used it on wax, but we do use it on 15-20 µm ground sections. Method for resin: * Pre-heat Sanderson's to 55-60°C. Stain sections for approx 20 minutes (check every 5 minutes). * Wash slides in warm water and dry with tissue * Counterstain with Acid Fuchsin solution for 1 ½ minutues. (2.5g acid fuchsin, 2.5ml glacial acetic acid, 500ml distilled water) * Drain slides and dry with tissue. I use this stain two ways routinely for thick or ground sections (35-50 microns) embedded in MMA DBP. You can stain with SRBS alone @ RT for 3-5 minutes after a brief acid etch in 0.7% FA for 30 sec (rinse in DI water and blot dry) or combine it with a Van Gieson picrofuchsin counterstain for 1-2 minutes (rinse in 100% EtOH and blot dry) after staining first in SRBS @ 60C for 10 minutes (rinse in DI water @ 60C and blot dry). I have never used or needed to use SRBS on thin plastic sections because I routinely use MacNeals tetrachrome combined with Von Kossa and a Modified Goldner's to obtain all my bone related information. With that said, I have also never used this stain on paraffin sections and would be interested in the results if you try it. Jack Ratliff I've used Sanderson's on plastic sections a lot in the past, works well. Recently I tried it on rat tibias, decaled, FFPE. Very disappointing. They look like Toluidine blue-- all shades of blue nothing else. Now I just stick with von kossa and/or tetrachrome for those. Kari ___ Suzanne Bruce, R.V.T. Histologist / Necropsy Coordinator Vet Path Services, Inc. (VPS) 6450 Castle Dr. Mason, OH 45040 Lab: (513) 469-0777 Fax: (513) 469-2474 Email: sbr...@vetpathservicesinc.com www.vetpathservicesinc.com http://www.vetpathservicesinc.com/ https://69.61.197.115/exchweb/bin/redir.asp?URL=http://www.vetpathservicesinc.com/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Staining racks
Hello, I work in plastics using Technovit 7200. We use plastic slides measuring 2x4cm. Does anyone else use slides this large and if so, what do you use as a staining rack or slide holder? They are too big to fit in the oversized glass slide racks. Thanks in advance for any help you can give me. Suzanne ___ Suzanne Bruce, R.V.T. Histologist / Necropsy Coordinator Vet Path Services, Inc. (VPS) http://www.vetpathservicesinc.com/ https://69.61.197.115/exchweb/bin/redir.asp?URL=http://www.vetpathservicesinc.com/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Sanderson's Rapid Bone Stain
We are planning on trying this stain on our plastics. Has anyone tried it on paraffin slides? If so, what were the results? Thanks in advance for any help. Suzanne ___ Suzanne Bruce, R.V.T. Histologist / Necropsy Coordinator ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Sanderson's Rapid Bone stain
Hello, I posted earlier regarding the Sanderson's Rapid Bone stain. I forgot to mention that I will be using this stain on 75-100 micron thick ground sections embedded in Technovit 7200 plastic. I am also wondering about paraffin staining using this stain. Here's one response so far: I've used Sanderson's on plastic sections a lot in the past, works well. Recently I tried it on rat tibias, decaled, FFPE. Very disappointing. They look like Toluidine blue-- all shades of blue nothing else. Now I just stick with von kossa and/or tetrachrome for those. Kari Any advice is welcome. Suzanne ___ Suzanne Bruce, R.V.T. Histologist / Necropsy Coordinator Vet Path Services, Inc. (VPS) 6450 Castle Dr. Mason, OH 45040 Lab: (513) 469-0777 Fax: (513) 469-2474 Email: sbr...@vetpathservicesinc.com www.vetpathservicesinc.com http://www.vetpathservicesinc.com/ https://69.61.197.115/exchweb/bin/redir.asp?URL=http://www.vetpathservicesinc.com/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Sanderson's Rapid Bone stain
The tissue in plastic will be rabbit femurs I believe. We haven't started anything yet, just getting references. As to the paraffin staining, I believe that was an overall question from my director to see if she could incorporate that stain in paraffin as well as our Technovit 7200 work. From: Jack Ratliff [mailto:ratliffj...@hotmail.com] Sent: Wed 2/18/2009 5:24 PM To: Suzanne Bruce; Histonet Subject: RE: [Histonet] Sanderson's Rapid Bone stain What is the tissue? What are your endpoints? Date: Wed, 18 Feb 2009 16:45:46 -0500 From: sbr...@vetpathservicesinc.com To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sanderson's Rapid Bone stain Hello, I posted earlier regarding the Sanderson's Rapid Bone stain. I forgot to mention that I will be using this stain on 75-100 micron thick ground sections embedded in Technovit 7200 plastic. I am also wondering about paraffin staining using this stain. Here's one response so far: I've used Sanderson's on plastic sections a lot in the past, works well. Recently I tried it on rat tibias, decaled, FFPE. Very disappointing. They look like Toluidine blue-- all shades of blue nothing else. Now I just stick with von kossa and/or tetrachrome for those. Kari Any advice is welcome. Suzanne ___ Suzanne Bruce, R.V.T. Histologist / Necropsy Coordinator Vet Path Services, Inc. (VPS) 6450 Castle Dr. Mason, OH 45040 Lab: (513) 469-0777 Fax: (513) 469-2474 Email: sbr...@vetpathservicesinc.com www.vetpathservicesinc.com http://www.vetpathservicesinc.com/ https://69.61.197.115/exchweb/bin/redir.asp?URL=http://www.vetpathservicesinc.com/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Plastics staining article
I keep seeing references to this article, but am having trouble finding it: Staining of plastic sections: a review of problems, explanations and possible solutions. RW Horobin, J Microscopy 131(2):173-186, 1983 Could someone point me in the right direction? Thanks in advance! Suzanne ___ Suzanne Bruce, R.V.T. Histologist / Necropsy Coordinator Vet Path Services, Inc. (VPS) 6450 Castle Dr. Mason, OH 45040 Lab: (513) 469-0777 Fax: (513) 469-2474 Email: sbr...@vetpathservicesinc.com www.vetpathservicesinc.com http://www.vetpathservicesinc.com/ https://69.61.197.115/exchweb/bin/redir.asp?URL=http://www.vetpathservicesinc.com/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
