Re: [Histonet] Filed slides sticking together
We leave ours in slide trays for at least 2 days prior to filing. We are a smaller derm lab so that’s probably not feasible for larger labs. Sent from my iPhone > On Apr 16, 2021, at 12:05 PM, Thomas Podawiltz via Histonet > wrote: > > I never have. Usually let them sit for two days before we filed them. > > > Sent from Yahoo Mail for iPhone > > > On Friday, April 16, 2021, 10:05 AM, Martha Ward-Pathology via Histonet > wrote: > > I am posting this question for our Histology manager: > > Does anyone dry coverslipped slides in an oven before filing and if so how > long and at what temperature. We are having issues with filed slides > sticking together. > > > Thanks in advance for your help with her question. > > Martha Ward, MT ASCP (QIHC) > Manager, Molecular Diagnostics Lab > Wake Forest Baptist Medical Center > Winston-Salem, NC 27157 > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Recommended thickness of Amyloid sections
I work at a Dermatology lab and our protocol for amyloid is 8-10 microns. Sent from my iPhone > On Apr 16, 2020, at 4:19 PM, Ken M via Histonet > wrote: > > Hello All. We have always cut all of our histology control slides at 5m. > We were told today that it is common practice to cut Amyloid at 8m? Is this > your experience? > > Ken > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Delay in embedding?
We have done this - only on an emergency basis. Sent from my iPhone > On Dec 17, 2019, at 2:48 PM, P Sicurello via Histonet > wrote: > > Good Morning Listers, > > > > How many out there will process tissue and then leave the cassettes at room > temperature and embed it at a later time (hours or the next day)? > > > > Please send me your opinions about doing this. I think it’s a bad idea, > others I speak with disagree. > > Sincerely, > > Paula Sicurello, HTL (ASCP)CM > > Histotechnology Specialist > > UC San Diego Health > > 9300 Campus Point Drive > > La Jolla, CA 92037 > (P): 858-249-5610 > > > > *Confidentiality Notice*: The information transmitted in this e-mail is > intended only for the person or entity to which it is addressed and may > contain confidential and/or privileged material. Any review, > retransmission, dissemination or other use of or taking of any action in > reliance upon this information by persons or entities other than the > intended recipient is prohibited. If you received this e-mail in error, > please contact the sender and delete the material from any computer. > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Air pocket in paraffin blocks
Oh no! Absolutely re-embed. Sent from my iPhone > On Feb 22, 2019, at 4:57 AM, Victoria Baker via Histonet > wrote: > > Yes, it does. The air bubble went directly under the tissue. > > Vikki > > On Thu, Feb 21, 2019, 8:43 PM Jennifer Phinney > wrote: > >> Vikki, >> Did the air bubble affect the section quality on the slide? If not, would >> it have been worth the delay to the case in order to melt and re-embed the >> tissue? >> >> I only re-embed blocks with air bubbles if it will affect the quality of >> the slide myself. >> >> >> Jennifer >> >> -Original Message- >> From: Victoria Baker via Histonet >> Sent: Thursday, February 21, 2019 4:49 PM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Air pocket in paraffin blocks >> >> Hi, >> >> When I was trained to do embedding there were many things that the >> professor stressed to me need to be done in order to have the tissue block >> acceptable for sectioning. One of these was air bubbles. >> >> Recently we had a new tech embed a derm block that had an bubble that was >> pretty big. The other (experienced) techs didn't think anything of it >> either and sectioned it. When I got the block for IHC screening I made a >> QA form stating that the block should have been re-embedded before giving >> it to be sectioned, or when the first tech sectioned it could have repaired >> the block or melted it down. This air bubble was big enough to be seen so >> I don't think it could have been missed - unless the block wasn't checked >> right after embedding. >> >> What has me a little upset is that no one seemed to care about this. >> >> I would really appreciate some feedback about this from other people. >> >> Thanks in advance. >> >> Vikki >> ___ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
