[Histonet] IHC on paraffin sections
Dear All, I want to stain for inos, Dectin-1 and Hem OX-1 on formalin fixed paraffins sections. I was told by some one these antibodies only work on frozen sections and not on paraffin sections, but I see all these antibodies datasheet say it work on paraffin sections as well. Any input will be helpful. Many Thanks, Kumar. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Immunofluorescence
Dear All, Can some one please send the protocol for immunofluorescence, I want to stain for FITC CD45.1 on mice spleens. Thank you very much for your help. Many Thanks, Anil kumar. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CD45.1 CD45.2 IF on parrafin sections.
Dear All, I want to do immunofluorescence on formalin fixed paraffin sections, Can you guys help me sending protocol how to do it ? Thank you very much for your help. BW Anil ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Endothelial histochemistry
Hi Ali, I tried FITC cd31 on mouse liver frozen section with tumors it worked really well. Good luck, Anil Kumar. On 22/10/10 11:29, Ali Nasr Esfahani alina...@student.liu.se wrote: Hi, I am just new in this group, so I hope this is the way I can get help. I am doing histochemistry on human hypothalamus and I need to stain endothelial cells. I tried UEA-I (lectin) and I got microvessels stained as the whole and like a line. But I would like to have an image of the endothelial cells of even larger vessels, the way that I can show cell by cell in a circular shape of a vessel. Could you help me and tell me what antibody or lectin helps me best. Regards Ali ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Necrotic and apoptotic cells
Dear All, I want to do look for Necrotic and apoptotic cells on liver tumor sections. What staining and antibodies are good for this technique?. Please let me know. Thank you very much in advance. Many Thanks, Anil Kumar. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Slide expiry
Dear All, I purchased bulk of Slides polysin coated slides and on the box it say they expire by end of this year, Does it really effect ?? Or I don't have to bother. Please let me know. Many Thanks, Anil Kumar. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] no subject
Dear All, I have a PFA fixed liver frozen sections and I want to stain for cd45.1, cd45.2 and f4/80. Can you guys please help me with the protocols for the staining. I would like to do Immunofluroscence. Thank you all in advance. Many Thanks, Anil Kumar. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC and IF
Dear All, I have kind of funny problem. I am doing IHC (substrate reaction suing Dabs reagent) and IF ( direct labeling of my monoclonal antibody with flurochrome). I am working on mouse liver frozen sections. When I did IHC the staining looks like cytoplasm and if I do IF for the same protein I saw nuclear staining. I am unable to conclude whether it is cytoplasm or nuclei staining. Please advice. The monoclonal antibody I used is not commercially available. We made it. Thanks In advance. Many Thanks, Anil Kumar. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC mouse antibody on mouse tissue
Hi All, Does any one got a nice protocol to do IHC using mouse monoclonal antibody on mouse tissue. Either frozen or paraffin section. Please let me know. Thanks in advance. ANIL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] no subject
Hi All, I have a problem with immunohistochmesitry. I am have raised my own antibody and it is not commercially available. This monoclonal antibody works fine for western blot. When it come to immunohistochemistry on mice liver frozen section, it is binding every where. I can see staining in my control as well. Any suggestion or guess's from you guys? Anil ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
