Re: [Histonet] Productivity Tracking

[WILLIAM DESALVO via Histonet]

s are experienced cutter who are sandbagging, they will
usually pick up their pace.

If someone is inexperienced and trying to get faster, that's OK, and you
can focus on trying to help them get faster.

A very small minority of people might be happy to sit there and cut their
routines as slowly as possible, and you have to decide whether or not to
start the process of getting rid of people like that. At least dividing the
cutting like this avoids them slowing down your workflow too much.

You can carrot and stick this however you want.  I see you are a histology
supervisor, so I'm assuming it's actually stick/stick. (j/k :))  Maybe not
let anyone go to lunch until all the cutting is done?  Let the fastest
cutters leave 30 min early on a Friday? Send a clear message that it's not
OK just to slack, you want everyone working to their potential.







On Fri, Jul 5, 2019 at 12:59 PM WILLIAM DESALVO via Histonet <
histonet@lists.utsouthwestern.edu<mailto:%0dhisto...@lists.utsouthwestern.edu>> 
wrote:

> I suggest you use slides created for the microtomy minimum standard. Not
> all blocks are created equal. A good target is 30 slides per hour, for
> mixed specimens. If all specialty, then adjust from the 30.
>
> William DeSalvo
> 
> From: Pairan, Kelly via Histonet
> Sent: Friday, July 5, 2019 8:41:28 AM
> To: 
> histonet@lists.utsouthwestern.edu<mailto:+histonet@lists.utsouthwestern.edu>
> Subject: [Histonet] Productivity Tracking
>
> Good Morning Histoland,
> How are you tracking your histotechs productivity when it comes to
> cutting?  We recently have implemented a 25 block per hour goal for all of
> our histotechs and are receiving some push back.  I made 25 block per hour
> the goal based on the following article that has been circulating for many
> years (
> https://www.researchgate.net/publication/41942535_Productivity_standards_for_histology_laboratories).
> While I do not want to compromise quality, we do have turnaround times to
> meet and I have experienced techs who are cutting less than 20 blocks per
> hour.  I understand that some tissues and protocols take longer so this is
> an average not something that has to be hit every shift.
>
> Thanks,
> Kelly
>
> Kelly Pairan,  HT (ASCP)CM, QIHC (ASCP)
> Histology Supervisor-Anatomic Pathology
> Department of Pathology and Laboratory Medicine
> Email:  
> kelly.pai...@nationwidechildrens.org<mailto:+kelly.pai...@nationwidechildrens.org>
> ph: 614-722-5414
> fx: 614-722-3033
>
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Re: [Histonet] Productivity Tracking

[WILLIAM DESALVO via Histonet]

I suggest you use slides created for the microtomy minimum standard. Not all 
blocks are created equal. A good target is 30 slides per hour, for mixed 
specimens. If all specialty, then adjust from the 30.

William DeSalvo

From: Pairan, Kelly via Histonet 
Sent: Friday, July 5, 2019 8:41:28 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Productivity Tracking

Good Morning Histoland,
How are you tracking your histotechs productivity when it comes to cutting?  We 
recently have implemented a 25 block per hour goal for all of our histotechs 
and are receiving some push back.  I made 25 block per hour the goal based on 
the following article that has been circulating for many years 
(https://www.researchgate.net/publication/41942535_Productivity_standards_for_histology_laboratories).
  While I do not want to compromise quality, we do have turnaround times to 
meet and I have experienced techs who are cutting less than 20 blocks per hour. 
 I understand that some tissues and protocols take longer so this is an average 
not something that has to be hit every shift.

Thanks,
Kelly

Kelly Pairan,  HT (ASCP)CM, QIHC (ASCP)
Histology Supervisor-Anatomic Pathology
Department of Pathology and Laboratory Medicine
Email:  kelly.pai...@nationwidechildrens.org
ph: 614-722-5414
fx: 614-722-3033

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Re: [Histonet] Histonet - Molecular Biology ASCP

[WILLIAM DESALVO via Histonet]

If you have the HTL, you are eligible to take the MB. You can also track with 
education and OJT for one year in molecular lab.

William DeSalvo

From: Joe Myers via Histonet 
Sent: Friday, May 31, 2019 4:15:01 PM
To: HistoNet
Subject: Re: [Histonet] Histonet - Molecular Biology ASCP

Diana:
Unfortunately, there’s no such certification for “molecular biology in 
histology” per se; the MB certification offered by the ASCP is generally 
pursued by individuals who are already certified as an MT or MLT since these 
types of procedures are usually performed in the ‘main lab’ (rather than in the 
histology section).  That’s not to say that folks with HT/HTL/CT certification 
shouldn’t consider pursuing the MB as well if they’d like.  As Mark indicated 
in his response, doing so usually involves taking several courses relating to 
MB techniques/instrumentation, and although you will occasionally see an MB 
‘overview’ course offered at a histology-society meeting, one would likely need 
a great deal more classroom/self-paced education and hands-on training to 
successfully pass the MB exam.
Cheers,
Joe Myers, M.S., CT(ASCP)QIHC



> Message: 1
> Date: Thu, 30 May 2019 18:13:11 +
> From: Diana Martinez-Longoria 
> To: "Histonet@lists.utsouthwestern.edu"
> Subject: [Histonet] Molecular Biology ASCP
> From: Diana Martinez-Longoria
> Sent: Thursday, May 30, 2019 11:09 AM
>
> Subject: Molecular Biology ASCP
>
>
>
> Good day,
>
>
> I don't know if anyone can help me regarding how does someone become ASCP 
> certified for Molecular Biology in Histopathology? Does anyone know how one 
> can study or take a program that can prepare you to take the ASCP Molecular 
> Biology Exam? Thank you !
>
>
> Diana Martinez-Longoria
>
> Bachelors of Science in Biology
>
> Histotechnician (ASCP)cm
>
> El Centro Regional Medical Center
>
> Phone: 760-339-7267
>
> Fax: 760-339-4570
>
> Email:dmlongo...@ecrmc.org
>


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Re: [Histonet] Tissue Tek TEC 6 Embedding Centre

[WILLIAM DESALVO via Histonet]

Check with the manufacturer as many in the pathology space are moving to 
placing 10 use and no longer supporting. As this continues to become more 
prevalent, the refurbish companies will continue to support. This is similar to 
the support of some clinical instruments.

William DeSalvo

From: E. Wayne Johnson via Histonet 
Sent: Thursday, May 30, 2019 7:13:02 PM
To: Etheridge, Sandra AGRI:EX; 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] Tissue Tek TEC 6 Embedding Centre

I am skeptical that parts are not available for these relatively new
(fifteen years old) machines...

Of course reliability is a serious concern in any case.

E. Wayne Johnson DVM
Enable AgTech
Beijing

Etheridge, Sandra AGRI:EX via Histonet wrote:
> Hi everyone,
>
> We are looking to purchase a new embedding centre and was wondering if anyone 
> has the new Tissue Tek TEC 6?  Our current Tissue Tek TEC is 15 years old and 
> parts are no longer available for repair.
>
> We are comparing the TEC 6 to the Leica HistoCore Arcadia system.  Just 
> wondering if anyone can give some feedback with regard to pro and cons of 
> either unit.
> Much appreciated!
>
> Sandra Etheridge
>
> BC Ministry of Agriculture
> Plant and Animal Health Centre
> Histology/IHC
> 1767 Angus Campbell Road
> Abbotsford, BC V3G 2M3
> # (604) 556-3120
>
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Re: [Histonet] Suggestions on a used microtome

[WILLIAM DESALVO via Histonet]

Contact Rankin for reliable refurbished instruments. I am partial to retracting 
microtones. Best to work with a company that provides quality

William DeSalvo

From: Craig via Histonet 
Sent: Tuesday, April 23, 2019 12:27:22 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Suggestions on a used microtome

Hi everyone!

Starting a new lab Texas and was looking at getting a used microtome and
wanted to see if any of you had suggestions on the best model to get used
and where?

Any suggestion will be greatly appreciated!

Best,
Craig
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Re: [Histonet] Fridge temp

[WILLIAM DESALVO via Histonet]

Simple, revalidate or pitch

William DeSalvo

From: MARY ANN via Histonet 
Sent: Tuesday, March 26, 2019 4:09:01 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Fridge temp

Let's say, hypotheticaly, if you discover your fridge with all you antibodies 
and detection kits were discovered to have been at 19c. for an undisclosed 
amout of time. 12 24 48 hours due tona power surge..south Florida weather.

Let's also propose your lab CFO/Owner dosent think its a big deal.

First how would you handle the issue given the frisge has been restored ?




Sent from Xfinity Connect Application
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Re: [Histonet] AB

[WILLIAM DESALVO via Histonet]

Helen, I have experience with both. Feel free to contact me directly with 
questions or we can arrange a call. More than happy to help

William DeSalvo
wdesalvo.cac@outlook. Com
480-622-1337

William DeSalvo


From: Helen via Histonet 
Sent: Thursday, November 29, 2018 9:10 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] AB


Hi there

I would like to know a Histology laboratory user experience of Tracking 
systems. Specifically vantage versus AB?
Any advice would be great.

Thanks
Helen



Helen Hegarty
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Re: [Histonet] AFB STAIN

[WILLIAM DESALVO via Histonet]

I completely agree with Tony. Not likely to see any sloughing off (shedding) of 
organisms from FFPE tissue blocks.

William DeSalvo


From: Tony Henwood (SCHN) via Histonet 
Sent: Thursday, October 4, 2018 1:54 PM
To: adesupo2...@hotmail.com; Laurie Colbert
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] AFB STAIN

Cross-contamination has not been proven in FFPE tissues.

I have not seen it in nearly 40 years of practice

Do you have any evidence of cross-contamination in FFPE?

Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principal Scientist, the Children’s Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA


From: Laurie Colbert via Histonet 
Sent: Thursday, October 4, 2018 10:36 PM
To: adesupo2...@hotmail.com; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] AFB STAIN

You should put the AFB control on a separate slide to prevent 
cross-contamination.

Laurie Redmond


-Original Message-
From: ADESUPO ADESUYI via Histonet 
To: histonet 
Sent: Wed, Oct 3, 2018 7:58 pm
Subject: [Histonet] AFB STAIN

Hello,
I have a question please. For the AFB Stain, do we put both the control tissue 
and the patient on the same slide?

Thanks,
Ade
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Re: [Histonet] Temperature for Storing Slides and Blocks

[WILLIAM DESALVO via Histonet]

I do not know of a standard, but you should make sure the storage temp is 5-10 
degrees lower(maybe more depending on fluctuations in temp for the area). That 
would be < 120F degrees. Request the location Facilities department understands 
you need temperature control

William DeSalvo


From: Dessasau III, Evan via Histonet 
Sent: Wednesday, September 5, 2018 11:52 AM
To: Blake Taylor
Cc: 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] Temperature for Storing Slides and Blocks

When we had our structure built we had cooling/heating units(2) put in a 
structure that is about 818sqft. 70F summer and 65 to 68F winter is what we aim 
for. Not sure if that is ideal but it keeps the blocks cool in the summer and 
the slides from sticking in the winter.
Thank you,
E-van
Histology Lab, Yerkes
Rm. 2122
7-7744 Lab
7-7902 office



-Original Message-
From: Blake Taylor via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, September 5, 2018 1:53 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Temperature for Storing Slides and Blocks

Is there a good reference or standard on what the room temperature should be 
for storing slides and blocks? We have moved our long term storage out to our 
hospitals warehouse and I believe the building is getting much too hot. What 
temperature range is everyone using?

Thanks so much

Blake Taylor
Surgical Pathology Supervisor
Lexington Medical Center
803-936-8214
bcdu...@lexhealth.org

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Re: [Histonet] Davidson

[WILLIAM DESALVO via Histonet]

Davidson’s fixative is suggested to for use to fix in up to 24 hours. The small 
nodes should be at least halved and larger nodes cut no more than 2-3 mm thick. 
There are a few papers that state “small biopsies can be fixed rapidly”. I 
suggest < 6 hours for small biopsies. I would do, minimally, a quick 
verification study on multiple tissue samples before risking diagnostic 
samples. Hope that helps.
William

William DeSalvo


From: Azam, Muhammad via Histonet 
Sent: Monday, July 30, 2018 10:55 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Davidson

Hi;

I am wondering what is the optimal time for Davidson's fixative (range). This 
is in regards to a sectioned lymph node.

Thanks;

Muhammad Azam, MD
Staff Pathologist
VHAMOU

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Re: [Histonet] Paperwork

[WILLIAM DESALVO via Histonet]

I am not a a big supporter of batch controls and managing that process.

Place a control section above patient sample, choosing 1 slide/case, prior to 
moving slides to stain. Control always gets to pathologist that is reading 
patient slides, assuming patient slides always get to pathologist and control 
is filed with the case.

Think through what are the opportunities for error and develop a change to 
either eliminate or reduce the opportunity.

There are multiple solutions to your problem and you will need to decide what 
process change gives you the results you desire. There should be multiple 
responses to facilitate your change.

William

William DeSalvo


From: Campbell, Tasha M. 
Sent: Friday, July 27, 2018 11:00 AM
To: WILLIAM DESALVO; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Paperwork

I am a little confused about your batch control explanation.  Do you mean to 
put a piece of control tissue on every case slide?




Tasha Campbell, B.S.,HTL(ASCP)
Frederick Gastroenterology Associates
310 W. 9th St.
Frederick, MD 21701
301-695-6800 ext. 144

From: WILLIAM DESALVO [mailto:wdesalvo@outlook.com]
Sent: Friday, July 27, 2018 1:20 PM
To: Campbell, Tasha M.; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Paperwork

I have a few suggestions:

Batch control - you do need to continue documentation of which cases/slides 
corresponds to the one control and be able to provide for inspection or 
re-review of a case. I suggest you consider taking pre-cut slide, add a new cut 
control section (1 per case if there are multiple slides) before staining. This 
conserves control tissue and removes some of the logistics of locating and 
matching batch control. I believe this will be a quality improvement without 
high cost.

Accessioning/ LIS - Ditch the log sheets and do not print, unless there is a 
specific need. All of your manual tracking process is now electronic. Do update 
all SOP’s and note date of change in process.

William

William DeSalvo


From: Campbell, Tasha M. via Histonet 
Sent: Friday, July 27, 2018 8:41 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Paperwork

Hi everyone,

I have 2 questions:


1. Could someone please share some ways to keep track of the control that goes 
with the slides that it was used for? So I am a small GI lab and there is a 
pathologist here a couple days a week. We do trichrome on Microscopic colitis 
cases and so I have been batching the trichromes because it's a long stain to 
do by hand and it's a lot easier to do it that way. But my slides are 
automatically printed for me and they have the date on them that the specimen 
was entered into the system. I cut the slide but then hold it until I am ready 
to do the stain. I put a date on the trichrome control slide but it of course 
does not match the date on the patient slides because they have been held for a 
few days. Is this something I even need to worry about? So far I have just been 
writing down the date that I stain the patient slide on a log sheet but I am 
trying to minimize the amount of paperwork/manual logging.


1. We recently got a accessioning system and I can now pull the number of 
blocks and stains done each day. Do I need to still keep writing down in my log 
sheet the number of blocks and stains? Do I need to print out the report that 
has the numbers and file it or since I have the ability to pull it from the 
system, I don't need to have physical logs.

I am just trying to minimize as much manual logging and paperwork as possible! 
Thanks in Advance!!!




Tasha Campbell, B.S.,HTL(ASCP)
Frederick Gastroenterology Associates
310 W. 9th St.
Frederick, MD 21701
301-695-6800 ext. 144

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Re: [Histonet] Paperwork

[WILLIAM DESALVO via Histonet]

I have a few suggestions:

Batch control - you do need to continue documentation of which cases/slides 
corresponds to the one control and be able to provide for inspection or 
re-review of a case. I suggest you consider taking pre-cut slide, add a new cut 
control section (1 per case if there are multiple slides) before staining. This 
conserves control tissue and removes some of the logistics of locating and 
matching batch control. I believe this will be a quality improvement without 
high cost.

Accessioning/ LIS - Ditch the log sheets and do not print, unless there is a 
specific need. All of your manual tracking process is now electronic. Do update 
all SOP’s and note date of change in process.

William

William DeSalvo


From: Campbell, Tasha M. via Histonet 
Sent: Friday, July 27, 2018 8:41 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Paperwork

Hi everyone,

I have 2 questions:


1. Could someone please share some ways to keep track of the control that goes 
with the slides that it was used for? So I am a small GI lab and there is a 
pathologist here a couple days a week. We do trichrome on Microscopic colitis 
cases and so I have been batching the trichromes because it's a long stain to 
do by hand and it's a lot easier to do it that way. But my slides are 
automatically printed for me and they have the date on them that the specimen 
was entered into the system. I cut the slide but then hold it until I am ready 
to do the stain. I put a date on the trichrome control slide but it of course 
does not match the date on the patient slides because they have been held for a 
few days. Is this something I even need to worry about? So far I have just been 
writing down the date that I stain the patient slide on a log sheet but I am 
trying to minimize the amount of paperwork/manual logging.


1. We recently got a accessioning system and I can now pull the number of 
blocks and stains done each day. Do I need to still keep writing down in my log 
sheet the number of blocks and stains? Do I need to print out the report that 
has the numbers and file it or since I have the ability to pull it from the 
system, I don't need to have physical logs.

I am just trying to minimize as much manual logging and paperwork as possible! 
Thanks in Advance!!!




Tasha Campbell, B.S.,HTL(ASCP)
Frederick Gastroenterology Associates
310 W. 9th St.
Frederick, MD 21701
301-695-6800 ext. 144

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Re: [Histonet] Coronary or Muscular Artery Histology Textbooks

[WILLIAM DESALVO via Histonet]

Stevens and Lowe, Histology, 4th edition, 2015. Good human anatomy  and 
descriptive

William DeSalvo


From: Vivian Hou via Histonet 
Sent: Thursday, July 26, 2018 11:40 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Coronary or Muscular Artery Histology Textbooks

Dear all,

I am searching for textbooks (not journal papers) focused more towards
coronary or muscular artery histology (or just vascular anatomy overall),
any suggestions you may have will be greatly appreciated!

Thank you all for your time,
Vivian

--
-
V Hou
Research Scientist | Engineer III
Center for CardioVascular Innovation
e: ho...@uw.edu | p: 206-221-3053
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Re: [Histonet] Water for H Stainers?

[WILLIAM DESALVO via Histonet]

Tap water is most often used in H staining for rinsing and sometimes blueing 
and can be very cost effective. I do suggest you use a filter prior to the 
water entering the instrument. The filter can eleviate one of the key concerns, 
contaminates. 
The typical contaminates/compounds can be: 
Inorganic ions: chlorides, nitrates, sulfates, sodium, calcium, iron
Organic molecules: humic acids, phenols, tannins, pesticide residues
Particles and colloids
Microorganisms and their by-products
Dissolved gases
 
The other key concern is that the pH of the tap water may vary by location and 
even season. To change the color of hematoxylin stained nuclei from redish to 
blue, the pH should be 7 or higher. Most tap water is typically slightly acidic 
(pH of around 6.0 to 6.8), but this is much more alkaline than the pH of the 
haematoxylin, around pH 2.7. Rinsing for two to five minutes in running tap 
water will remove most of the excess mordant giving sharp blue nuclear 
staining. If you want sharper and better defined
 
As my good friend Skip Brown states, a good H stain is "balance of 
coloration". Understand the chemistry and you control the balance. 
 
William DeSalvo, BS HTL(ASCP)
 
> Date: Tue, 27 Sep 2016 14:17:08 -0700
> To: l...@premierlab.com; pat...@gmail.com; histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] Water for H Stainers?
> From: histonet@lists.utsouthwestern.edu
> 
> Our Prisma is connected to tap water with a prefilter that we change weekly. 
> CathyBritish Columbia 
> 
> 
> Sent from my Samsung Galaxy smartphone.
>  Original message From: Elizabeth Chlipala via Histonet 
>  Date: 2016-09-27  9:54 AM  (GMT-08:00) 
> To: P Sicurello , "'histonet@lists.utsouthwestern.edu'   
> (histonet@lists.utsouthwestern.edu)"  
> Subject: Re: [Histonet] Water for H Stainers? 
> Paula
> 
> We have ours hooked up to tap water.
> 
> Liz
> 
> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
> Premier Laboratory, LLC
> PO Box 18592
> Boulder, CO 80308
> (303) 682-3949 office
> (303) 682-9060 fax
> (303) 881-0763 cell
> l...@premierlab.com
> www.premierlab.com
> 
> Ship to Address:
> 
> Premier Laboratory, LLC
> 1567 Skyway Drive, Unit E
> Longmont, CO 80504
> 
> -Original Message-
> From: P Sicurello via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
> Sent: Tuesday, September 27, 2016 10:28 AM
> To: HistoNet
> Subject: [Histonet] Water for H Stainers?
> 
> Good Morning Everyone,
> 
> 
> 
> What type of water do you use with your automated H stainers?  House or 
> deionized/distilled?
> 
> 
> 
> We cannot buy one of the waterless, fancy schmancy H stainers at this time. 
>  L Thank you in advance.
> 
> Sincerely,
> 
> 
> 
> Paula
> 
> 
> 
> Paula Sicurello, HTL (ASCP)CM
> 
> Histotechnology Specialist
> 
> UC San Diego Health
> 
> 200 Arbor Drive
> 
> San Diego, CA 92103
> 
> (P): 619-543-2872
> 
> 
> 
> *Confidentiality Notice*: The information transmitted in this e-mail is 
> intended only for the person or entity to which it is addressed and may 
> contain confidential and/or privileged material.  Any review, retransmission, 
> dissemination or other use of or taking of any action in reliance upon this 
> information by persons or entities other than the intended recipient is 
> prohibited.  If you received this e-mail in error, please contact the sender 
> and delete the material from any computer.
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Re: [Histonet] No More Blog Posts -- Over and Out!

[WILLIAM DESALVO via Histonet]

Another personal e-mail from you that is unwanted. I am feeling like I have my 
own stalker. You are one of the reasons that individuals will not post or 
comment, personal and ugly. I cannot imagine why your workplace (LUMC.edu) 
chose you to represent them. I request that you no longer contact me and make 
personal attacks. I know nothing of you and you certainly do not know anything 
about me. If you have something to say, post to the listserve, not me.

> From: spinhe...@lumc.edu
> To: wdesalvo@outlook.com
> Subject: RE: [Histonet] No More Blog Posts --  Over and Out!
> Date: Mon, 2 May 2016 20:15:38 +
> 
> As is yours. Just can't keep that dictatorial aspect out of your charming 
> consultant two cents worth.
> 
> Steve Pinheiro
> 
> 
> -Original Message-
> From: WILLIAM DESALVO via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
> Sent: Monday, May 02, 2016 2:11 PM
> To: Manfre, Philip; Rene J Buesa
> Cc: 'histonet@lists.utsouthwestern.edu'
> Subject: Re: [Histonet] No More Blog Posts -- Over and Out!
> 
> Your response is WAY out of line. Keep it professional.
> 
> Sent from my Windows Phone
> 
> From: Manfre, Philip via Histonet<mailto:histonet@lists.utsouthwestern.edu>
> Sent: ‎5/‎2/‎2016 12:08 PM
> To: Rene J Buesa<mailto:rjbu...@yahoo.com>
> Cc: 
> 'histonet@lists.utsouthwestern.edu'<mailto:histonet@lists.utsouthwestern.edu>
> Subject: Re: [Histonet] No More Blog Posts --  Over and Out!
> 
> Delete button broken, huh.  I suppose it was necessary to attack a colleague 
> to feel puffed up about yourself.  Bully for you!
> 
> -Original Message-
> From: Rene J Buesa via Histonet [mailto:histonet@lists.utsouthwestern.edu]
> Sent: Monday, May 02, 2016 2:23 PM
> To: Lester Raff MD; 'histonet@lists.utsouthwestern.edu'
> Subject: Re: [Histonet] No More Blog Posts -- Over and Out!
> 
> Thank you VERY MUCH!René
> 
> On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet 
> <histonet@lists.utsouthwestern.edu> wrote:
> 
> 
>  To My Lab Colleagues:
> 
> As my intent has never been to sow discontent or rancor, I think it is for 
> the best if I no longer post links to my blog, lab related or otherwise.  Of 
> course the blogs go on, and if anyone is interested in being added to my 
> mailing list for future notifications, just drop me a line at 
> les.r...@post.com<mailto:les.r...@post.com>  The mailing list is never used 
> for any purpose other than announcing a new blog post. Be sure to let me know 
> you are from the Histonet list!
> 
> I will continue to participate in any histology/pathologist discussions here 
> as I have for many years.
> 
> Cheers,
> 
> Lester J. Raff, MD MBA
> UroPartners
> Medical Director Of Laboratory
> 2225 Enterprise Dr. Suite 2511
> Westchester, Il 60154
> Tel: 708-486-0076
> Fax: 708-492-0203
> 
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> 
> 
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Re: [Histonet] No More Blog Posts -- Over and Out!

[WILLIAM DESALVO via Histonet]

Your response is WAY out of line. Keep it professional.

Sent from my Windows Phone

From: Manfre, Philip via Histonet
Sent: ‎5/‎2/‎2016 12:08 PM
To: Rene J Buesa
Cc: 
'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] No More Blog Posts --  Over and Out!

Delete button broken, huh.  I suppose it was necessary to attack a colleague to 
feel puffed up about yourself.  Bully for you!

-Original Message-
From: Rene J Buesa via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Monday, May 02, 2016 2:23 PM
To: Lester Raff MD; 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] No More Blog Posts -- Over and Out!

Thank you VERY MUCH!René

On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet 
 wrote:


 To My Lab Colleagues:

As my intent has never been to sow discontent or rancor, I think it is for the 
best if I no longer post links to my blog, lab related or otherwise.  Of course 
the blogs go on, and if anyone is interested in being added to my mailing list 
for future notifications, just drop me a line at 
les.r...@post.com  The mailing list is never used for 
any purpose other than announcing a new blog post. Be sure to let me know you 
are from the Histonet list!

I will continue to participate in any histology/pathologist discussions here as 
I have for many years.

Cheers,

Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203

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proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
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please notify us immediately by reply e-mail and then delete it from
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Re: [Histonet] Medical/health related post

[WILLIAM DESALVO via Histonet]

Again, why the non-Histo post. Take this to another source. I do not understand 
why Dr. Raff has not been removed from this list serve. This is a valuable site 
for histotechnolgy related issues, please let us keep it that way.

Sent from my Windows Phone

From: Lester Raff MD via Histonet
Sent: ‎4/‎28/‎2016 6:59 AM
To: 
'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Medical/health related post

http://www.chicagonow.com/downsize-maybe/2016/04/running-mates-and-other-mates-fighting-trump-fighting-cancer/

Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203

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Re: [Histonet] Passing the HTL

[WILLIAM DESALVO via Histonet]

Congratulations! Well done and now the fun begins.

Sent from my Windows Phone

From: Terri Braud via Histonet
Sent: ‎4/‎26/‎2016 10:58 AM
To: 
'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] Passing the HTL

BIG Congratulations! To Patrick Lewis on passing your HTL

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874

7. I did it  I am now a certified HTL (Lewis, Patrick)
Message: 7
Date: Tue, 26 Apr 2016 16:30:28 +
From: "Lewis, Patrick" 
Subject: [Histonet] I did it  I am now a certified HTL
Hi Everyone
After years of putting it off, I finally took my ASCP HTL  exam and passed it.
Huzzah!
Patrick.
Patrick Lewis
Research Associate II Bench
Seattle Childrens Research Institute
206-884-1115



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Re: [Histonet] Blog Post Not lab related

[WILLIAM DESALVO via Histonet]

IMO, this is a list serve not an advertising space. Keep blog posts and other 
advertising off the list. Keep the exchange about histotechnology. Plenty of 
interweb space for other communication. Dr. Raff and everyone is always welcome 
to be part of the discussion and help get answers to those that need.

Sent from my Windows Phone

From: Megan Dishop via Histonet
Sent: ‎4/‎14/‎2016 2:01 PM
To: 
histonet@lists.utsouthwestern.edu; 
heather.brow...@us.af.mil
Subject: Re: [Histonet] Blog Post Not lab related



Thank you for bringing attention to this, Heather. This has been my
observation also, and I couldn't agree more. Let's keep it professional.


Sent from my Sprint Samsung Galaxy® Note 4.

 Original message 
From: "BROWN, HEATHER L GS-07 USAF AETC 59 CRD/SGVUO via Histonet"

Date: 4/14/16  1:02 PM  (GMT-06:00)
To: histonet@lists.utsouthwestern.edu, HEATHER L GS-07 USAF AETC 59
CRD/SGVUO BROWN 
Subject: Re: [Histonet] Blog Post Not lab related


>>> "BROWN, HEATHER L GS-07 USAF AETC 59 CRD/SGVUO via Histonet"
 2016-04-14T13:02:26.146402 >>>
I am new to the Histonet, not to histology though.  There have been 2
great question and answer sessions since I've been on here.  The way
some of you guys talk to each other is so disrespectful.  I went through
AFIP as a civilian years ago and I thought the histo world was a small
society of people who shared a common, if not morbid, curiosity of the
human anatomy, how it works and how can we do the best we can do to help
our patient.  I will read all the submissions, because there may
actually be a good answer to a question.  I will have to really weigh my
options with asking a question...cruelty and ridicule or the possibility
of a good answer?  If you don't like histo, then don't do histo.  If you
can't be nice, then say nothing at all.  Life is a beat down everyday, I
darn sure wouldn't need if from my peers too.

Heather L. Brown, HT, ASCP
JBSA-Lackland, Texas



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Re: [Histonet] Processing issues

[WILLIAM DESALVO via Histonet]

Not knowing what issues you have, I suggest you look to your samples for 
processing. Reduce thickness and the shorter times will work. 2-2.5 mm thick. 
Standardize fixation before placing in tissue processor. What time is the 
processor started and what time does the tech remove? You may be able to reduce 
delay and increase alcohols. Specimen thickness will reduce the the time for 
solution to move through the tissue sample and allow exchange of solution. 
There are many options, but be more specific about the problem and you can 
narrow the changes

Sent from my Windows Phone

From: Charles Riley via Histonet
Sent: ‎3/‎22/‎2016 8:57 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Processing issues

We are having issues with our tissue processing. We use the thermo shandon
excelsior processor. All steps have agitation and under vacuum. Our routine
process is  as follows

1. Formalin   1hr
2. Formalin   1hr
3. 75% alcohol 30 mins
4.85% alcohol 30 mins
5. 95% alcohol 30 mins
6.95% alcohol 30 mins
7. 100% alcohol 30 mins
8. 100% alcohol 30 mins
9 xylene 30 mins
10. xylene 30 mins
11 xylene 30 mins
12. Wax for 30 mins
13. Wax 30 mins
14. Wax 30 minutes.

Can anyone give any suggestions for altering this process to work better? I
know the process should probably be longer however medical director does
not want to delay turn around time. If possible keep the process to under 9
hours. If this isn't conducive to consistent processing please explain why
so  I can show my superiors to give myself some leverage to change things
--

Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs

Doctors Pathology Services, Dover DE
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Re: [Histonet] automatic stainers and tissue processors

[WILLIAM DESALVO via Histonet]

I suggest you take the opportunity to find the instruments that fit your needs. 
Are going to rapid process, what types of tissue samples do you have, what do 
you want to improve? The histology lab is changing and you should look 5+ years 
down the road. I would not want you to be pigeon holed into a set of 
instruments. Drive quality, patient experience and flexibility. I am a wrong 
proponent of rapid processing, fix appropriately and do not use xylene or over 
process. For staining, Prisms and film are my choices. You need to find the 
instrumentation that works best for you. Good luck in your search, there are 
many options and great products to evaluate.

Sent from my Windows Phone

-Original Message-
From: "Silvia Bonner via Histonet" 
Sent: ‎2/‎29/‎2016 1:19 PM
To: "histonet@lists.utsouthwestern.edu" 
Subject: [Histonet] automatic stainers and tissue processors


Hello,
We are looking into purchasing new automatic H strainers and new tissue 
processors.  Any helpful advice?  I know this may have been discussed before 
but I an new to histonet.
Thanks for your help!
Silvia Bonner, HT(ASCP) CM
Histology Supervisor

sbon...@pathregional.com
Pathologists' Regional Laboratory
1225 Highland Ave
Clarkston, WA 99403
 This email may contain physician, patient and/or attorney material that is 
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Re: [Histonet] Bar Code and Tracking

[WILLIAM DESALVO via Histonet]

I believe it will be difficult to afford the cost of Co-Path in your small lab 
area, unless you can attach to a bigger hospital information system contract. I 
suggest you look to companies that have developed products for the small to 
medium sized histology labs.

Sent from my Windows Phone

From: Abbott, Tanya via Histonet
Sent: ‎2/‎17/‎2016 11:48 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Bar Code and Tracking

Hello,
I manage a small Pathology lab where we generate approx. 80-140 blocks for 
Histo and 5-6 Cytology fluids a day. I have 2 Histotechs that swap doing 
grossing monthly. I have 1 full time and 1 part time Cytotech and a Cyto 
assistant. I am curious if there are any smaller labs out there that have gone 
to a Bar Code and Tracking system and can offer any input. I came from a much 
larger lab that used CoPath from accessioning to sign-out, so that is the kind 
of system I am used to. It is certainly a system I wish to integrate in to my 
lab on a much smaller level, we currently have a very manual process, other 
than some entry/labeling in our LIS.
Any input is appreciated! Tanya

Tanya G. Abbott
Pathology Manager
Penn State Health St. Joseph
Pathology Department
(o) 610-378-2635
(f) 670-898-5871
tabb...@pennstatehealth.psu.edu
Penn State Health St. Joseph | The Future of 
Healthcare

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Re: [Histonet] Storage of Unstained Slides

[WILLIAM DESALVO via Histonet]

I would not heat dry unstained as heat drying removes the paraffin and 
cells/tissue in no longer protected by the paraffin. Drying slides is a process 
to remove the sate trapped between the section and glass. Heat drying can speed 
that process and is also helpful in "baking" tissue to slide to assist in 
tissue adherence. Air dry until staining is requested, is my advice.

Sent from my Windows Phone

From: T Williams via Histonet
Sent: ‎2/‎1/‎2016 5:31 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Storage of Unstained Slides

Does anyone have any suggestions as to how unstained slides with patient
tissue should be stored?  Or could anyone recommend a product for
storage?   Also - is it preferable that these slides not be placed in the
dryer until they are intended to be stained?  If so, what is the problem
with baking them as soon as they're cut and dried at room temperature?

I apologize for all the questions but I do  appreciate the guidance.

T. Williams
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Re: [Histonet] changing tissue processors

[WILLIAM DESALVO via Histonet]

I would suggest by cassette numbers. It will take some data collection, but 
once you settle on the number of cassettes, then you will have the process in 
control. The actual number will depend on the tissue types placed in the 
cassettes and the amount of blood, lipids and other elements extracted. Start 
with a minimum of 600 cassettes and see see what additional cassettes can be 
processed without losing quality.

Sent from my Windows Phone

From: Nirmala Srishan via Histonet
Sent: ‎1/‎11/‎2016 12:33 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] changing tissue processors

Hi Histonetter,


Does any one have guidelines, which indicates how often to change the
tissue processors - Cassette Volume run, Make of processors etc

Thank you


Nirmala Srishan
Histology Supervisor
Holy Name Medical Center
718 Teaneck Road
Teaneck NJ 07666
Lab: 201 833 3023
Office: 201 541 6328







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[Histonet] NSH Financial and Tax Statements

[WILLIAM DESALVO via Histonet]

As a due paying member of NSH, I ask all members and anyone interested in 
Histotechnology request from NSH office to see the Financial and Tax Statements 
for 2013 / 2014. This is public information. We should all know where our dues 
money, money from educational activities and vendor money from the convention 
is being spent. 

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Re: [Histonet] Help with causes or theory behind failure of nuclear counterstaining after IHC!

[WILLIAM DESALVO via Histonet]

Maria, I. Think you may have a pH issue. High pH results in reduction of 
protons, H+, effects dye structure and can cause light to no staining after 
bluing. If you are using hematoxylin w/ aluminum, most popular, decreased pH = 
decreased intensity. Acid breaks the Al+3. Check your ph throughout the 
process. Sounds like something has changed. Good luck.

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From: Morken, Timothy via Histonet
Sent: ‎1/‎4/‎2016 9:20 AM
To: Maria Mejia
Cc: Histonet
Subject: Re: [Histonet] Help with causes or theory behind failure of nuclear 
counterstaining after IHC!

Maria,

If the counterstain is good when done before IHC stain and poor after it sounds 
like proteins are being extracted during the IHC processing and staining. Have 
you tried staining sections after each step of the IHC process to isolate the 
point the stain becomes weak?

Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center



-Original Message-
From: Maria Mejia via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Sunday, January 03, 2016 8:29 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Help with causes or theory behind failure of nuclear 
counterstaining after IHC!

Happy New Year Everyone,

I'm the lead histologist working in an IHC based research lab focused on early 
stages of Alzheimer's disease.  We work on paraffin sections processed & cut 
from 600um celloidin sections.  Including a lot of 60um cellodin sections from 
whole human brainstem.

For years, everything has going good regarding counterstaining after single & 
double IHC staining on 60um free-floating sections.  However for the past two 
months we've struggled to achieve good visible counterstaining on IHC sections 
to count the stained neurons - to see clearly the nucleus & nucleolus!

For a number of years, Gallocyanine was our choice of counterstain after IHC.  
Now, it's NOT working (neurons not stained visible enough to count).  We've 
also tried cresyl violet
counterstain -  staining too weak!   In both counterstains, we modified
the staining protocols quite a number of times to get good visible staining - 
nothing!!!

Strangle because we get lovely counterstained neurons with NO IHC staining on 
our 60um sections, but as soon as we take the sections through the IHC protocol 
e.g. antigen retrieval, antibodies & chromogens - counterstain is too weak!  
Our paraffin IHC sections work & look wonderful!

Now, my PI wants to try methyl green counterstain, however I think we'll have 
the same problem.
Here's what I need help with:

1) Can someone please explain the reason or theory  behind the failure of 
counterstain uptake by cells such as human neurons on 60um celloidin sections?

2) Can anyone please offer staining protocols that use alternative dehydration 
& clearing reagents.
I've been using alcohols dehydration (96% & 100%) without success as well as 
clearing with xylene - which hardens the tissue if left too long in this 
reagent.

 I was thinking of perhaps using acetone instead of alcohols & maybe using a 
methyl salicylate or chloroform.  Thoughts anyone?

I wish Dr Chris van der Loos was still with us.  I'd dearly like to hear from 
anyone who can help with this issue - Gayle Callis, Terry Johnson, Dr Hohn 
Kiernan et al.

Any assistance anyone can provide will be greatly appreciated!

Best
Maria Mejia
UCSF
Memory & Aging Department
San Francisco, CA
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Re: [Histonet] Collodian bag for cell blocks

[WILLIAM DESALVO via Histonet]

What method are you using? Making your own coated tubes, UCSF or MD Anderson. 
Are you purchasing coated tubes? Once the tube is coated, dried (using hood) 
and filled w dH2O, there should be no odor. Always work w/ collodion under the 
hood. Ethyl ether is main component. Once in cassette and processed, there 
should not be odor.

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From: Baldwin, Kathy via Histonet
Sent: ‎1/‎4/‎2016 2:07 PM
To: 
'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Collodian bag for cell blocks

Hi All
Was wondering if anyone is using the Collodian bag for cell blocks.  We just 
got it and have been using it 'works great' but all the techs are complaining 
of the smell.. Our ventilation has been looked at and has passed however the 
smell still lingers in the room.  Any suggestions??


Thanks
S. Kathy Baldwin
Histology/Cytology Supervisor
Memorial Hospital and Health Care Center
800 West 9th St.
Jasper, Indiana  47546
Office 812-996-0210
Fax 812-996-0232
Cell 812-887-3357





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Re: [Histonet] Best solution for surface decalcification of paraffin block

[WILLIAM DESALVO via Histonet]

I do no recommend surface decal, but if you must use a formic acid product. 
Rinse the block several times to remove decal before cutting a section. Always 
better to go to the source and adjust time in decal and method for determining 
end point before processing.

Sent from my Windows Phone

From: Carlos Defeo via Histonet
Sent: ‎12/‎16/‎2015 8:37 PM
To: 
histonet-requ...@lists.utsouthwestern.edu
Cc: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Best solution for surface decalcification of paraffin block

Hi all, I would like your opinion on the best way to achieve many few
decent sections of calcified material.
IED by Biocare,a strong acid solution, any input would be greatly
appreciated.
Thanks in advance.
Carlos Defeo
Histotechnologist
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Re: [Histonet] Workload

[WILLIAM DESALVO via Histonet]

I measure productivity by unit per task (specimens, blocks, slides). There is 
no right or wrong, but there are multiple tasks associated w/ a CPT code. I 
suggest you talk to you management or finance and find out what they use to 
measure productivity. Using their method/units allows for roll up to other 
reports.

Sent from my Windows Phone

From: Manahil via Histonet
Sent: ‎12/‎8/‎2015 12:13 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Workload

Hi histonet,
I have a query about workload and how do you measure productivity? Do you 
capture it by CPT codes?
Your input is highly appreciated,

Manahil
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Re: [Histonet] Rapid tissue Programs

[WILLIAM DESALVO via Histonet]

First suggestion is to remove the sponge. There will be carry over on 
biopsy/rapid tissue processor schedules. The sponges do require longer times to 
drain and exchange liquids. Your fixation times must be >6 hours. Make sure you 
are not applying too much heat to processing. Small biopsies are delicate and 
exchange of fluids/paraffin should not need physical elements to assist. 
Additionally, do not over dehydrate through alcohols.  You processor schedule 
will need to be validated, if you make time/ pressure/heat or reagent change. 
Good luck in the problem solving process.

Sent from my Windows Phone

From: Vickroy, James via Histonet
Sent: ‎11/‎24/‎2015 10:27 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Rapid tissue Programs

Our latest CAP survey was returned today and although there are no major issues 
one possible improvement area is evident.   On all of the biopsies the area of 
"fixation/processing" was not rated as excellent.  The suggested possible 
reasons were:   fixation incomplete, nuclear bubbling artifact, tissue poorly 
processed.   The survey also said that many of the samples sent in throughout 
the country had similar  issues in the fixation/processing area most likely 
because of the rapid turnaround times and shortened processing times.   I am 
trying to be proactive here and see if we can adjust some times to improve the 
processing quality even though we have not had any complaints from the 
pathologists.   Of course we all know that other artifacts caused prior to the 
specimen arriving in the lab can also have an effect on the quality of the H 
slides.   Our fixation times should not be a factor so I have to conclude that 
maybe the rest of our processing times need to be adjusted.   Another factor 
that we have is that we use blue sponges for almost all of our tissues.  Our 
largest number of specimens are GI biopsies.If possible can anyone share 
with me their rapid processing schedules or simply the approximate times they 
have for each dehydration or clearing step. We do run a larger overnight tissue 
run on any biopsy or tissue that we feel is too large for the "rapid run".I 
am hesitant to run the biopsies routinely on the longer programs becase of over 
dehydration, etc. even though we do use an alcohol blend.

Any suggestions or similar experiences please share.Again our pathologists 
say everything looks great so I don't want to change much.

Jim

Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois  62703
Office:  217-528-7541, Ext. 15121
Email:  jvick...@springfieldclinic.com



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Re: [Histonet] Xylene and Formalin substitutes

[WILLIAM DESALVO via Histonet]

Contact Ada Feldman, Anatech Ltd. Best resource.

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From: Bharti Parihar via Histonet
Sent: ‎10/‎21/‎2015 3:49 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Xylene and Formalin substitutes

Hello everyone. I'm interested in starting a conversation at my workplace
regarding substitutes for xylene,  and if there are any substitutes for
formalin. If anyone out there is using any can they please give me feedback
regarding the following questions,

1. Name of substitutes?
2. Pros and Cons?
3. Any quality issues when performing H, IHC or Special Stains?
4. Is it cost effective?
5. Does it require different dehydrants other than alcohol?
6. If formalin is still used as fixative,  what xylene substitute works
best with formalin?

I appreciate any feedback! Thanks!
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Re: [Histonet] dictation systems

[WILLIAM DESALVO via Histonet]

At our lab, we have used a digital server system called Fusion.

I suggest you go digital and then consider if you can also move to voice 
recognition and activation for efficiency.

There are so many more options now as compared to the tape systems.

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From: Horn, Hazel V via Histonet
Sent: ‎9/‎17/‎2015 9:00 AM
To: histonet 
(histonet@lists.utsouthwestern.edu)
Subject: [Histonet] dictation systems

All,
We are looking for a new dictation system for grossing.  Will you please share 
what you use?
We still have a Lanier microcassette system and it's outdated and not made 
anymore.
Thanks.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org







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