Re: [Histonet] Microwave Tissue Processors

[Willis, Donna G via Histonet]

Who is the manufacturer of the unit that you are using?

Donna Willis HT/HTL (ASCP)
Anatomic Pathology Manager
Baylor Scott&White Health
Baylor University Medical Center
3500 Gaston Ave.
Dallas, Texas 75246
214-820-2465 office
214-725-6184 cell

-Original Message-
From: Robert Herrera via Histonet  
Sent: Friday, November 17, 2023 12:08 PM
To: histonet@lists.utsouthwestern.edu
Subject: {EXTERNAL} [Histonet] Microwave Tissue Processors


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Quick question; are there any facilities utilizing “microwave tissue 
processors?” If so, can I get the name and number of the company that is 
servicing the microwave. We have three that need servicing.
Thank YouRobert

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Re: [Histonet] Leica H&E 600

[Willis, Donna G via Histonet]

Good Morning Beth,
The HE600 is a Roche product.  The slides are stained flat with reagents being 
applied to each slide individually instead of batch staining like other 
instruments.  This allows you to get the same staining quality on EACH slide.   
We have used the instrument in our lab since 2005 and got a second unit when we 
moved into a larger location 2 years ago.  Coverslips are removed by apply heat 
from a hot plate and we have no issues with the hematoxylin if we remove a 
coverslip.  We rarely have issues with coverslips that we need to remove one.  
Let me know if you have any other questions.

Donna Willis HT/HTL (ASCP)
Anatomic Pathology Manager
Baylor Scott&White Health
Baylor University Medical Center
3500 Gaston Ave.
Dallas, Texas 75246
214-820-2465 office
214-725-6184 cell

-Original Message-
From: O'Neil, Beth via Histonet  
Sent: Wednesday, August 23, 2023 7:27 AM
To: Histonet 
Subject: {EXTERNAL} [Histonet] Leica H&E 600


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I would appreciate any feedback from anyone with experience using the Leica H&E 
600. I recently attended a demo of the unit and we are still hung up on the 
large slide tray it uses instead of the traditional slide racks.   I also spoke 
with another user who indicated that if you need to re-coverslip a slide it is 
very difficult to remove the existing coverslip and when you do the hematoxylin 
is removed from the stain ???  We already know we will need two units to handle 
our workload of approximately 900 H&E.  Any information would be greatly 
appreciated.

Beth O'Neil
beth.one...@wvumedicine.org
West Virginia University, JW Ruby Memorial Hospital Confidentiality Notice: 
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[Histonet] DreamPath Block and Slide Storage Systems

[Willis, Donna G via Histonet]

Is there anyone on the Histonet that is currently using the DreamPath Block and 
Slide Storage System?  Can you give me your opinion of the company and the 
product.

Thanks,

Donna Willis HT/HTL (ASCP)
Anatomic Pathology Manager
Baylor Scott&White Health
Baylor University Medical Center
3500 Gaston Ave.
Dallas, Texas 75246
214-820-2465 office
214-725-6184 cell

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Re: [Histonet] IHC gunk

[Willis, Donna G via Histonet]

What instrumentation are you using.  Have you talked to your vendor?

Donna Willis
Anatomic Pathology Manager
Baylor Scott&White Health
Baylor University Medical Center
3500 Gaston Ave|Dallas, Texas 75246
214-820-2465 office|214-725-6184 mobile



-Original Message-
From: Karen Heckford CA-San Francisco via Histonet 
 
Sent: Wednesday, March 22, 2023 12:45 PM
To: Histonet 
Subject: {EXTERNAL} [Histonet] IHC gunk


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Good Morning,
I have recently developed a problem with contamination of some kind on my IHC 
slides.
The contamination is black clumps and lays on top of the tissue.  I have
been told it is bacteria but the Pathologist and I kind of doubt that.   I
cannot get a good picture of it to show.  It may not be on all the slides on 
the same run or even the same antibody slide.

I have decontaminated the whole system.  I have put fresh reagents on the
instrument.   This stuff looks like it would wash off at the end of the run
since it is sitting right on top of the tissue.  I do not see this stuff
elsewhere on the slide, only the tissue.   Does not matter if the tissue
was cut fresh or not.

I have tried everything I can think of to get rid of it and still have this 
issue.  I use DiH20 from the hospital system.  Not sure if this may be the 
problem.  I can have Engineering test the DiH20.

Any help would be greatly appreciated.

Thanks,

Karen Heckford HT ASCP CE

Lead Histology Technician

St. Mary's Medical Center

450 Stanyan St.

San Francisco, Ca. 94117

415-750-5751

karen.heckford@ commonspirit.org




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Re: [Histonet] Tracking of blocks onto processors

[Willis, Donna G via Histonet]

Chris,
We have been tracking blocks since we went live with Vantage over 10 years ago. 
 Roche (Ventana then) built a custom program for us to use at that time because 
they had not yet implemented it into Vantage.  In the past 2 years Roche has 
updated their software to allow documenting this information.  There are 
several options of how the scanning is performed.  For our lab we scan before 
we place the block into the formalin holding tanks, right after the 
PA/Resident/Grossing Tech have placed the tissue into the block.  I prefer it 
done there because I do not want my technicians exposed an extra time to 
formalin if the scanning is done right before the baskets are placed on the 
processors.  We have a manual log that is used to document the time the 
cassette are placed in and taken out of the processors.  We also track our 
blocks and slides into storage.  I hope this helps.  

Donna Willis
Anatomic Pathology Manager
Baylor Scott&White Health
Baylor University Medical Center
3500 Gaston Ave|Dallas, Texas 75246
214-820-2465 office|214-725-6184 mobile



-Original Message-
From: Hagon, Christopher (Health) via Histonet 
 
Sent: Tuesday, March 21, 2023 5:17 PM
To: histonet@lists.utsouthwestern.edu
Subject: {EXTERNAL} [Histonet] Tracking of blocks onto processors


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UNOFFICIAL

Hi Histonetters,

Just wondering if or how others are tracking/scanning racks of blocks onto 
processors. In this day and age of traceability with barcodes, we scan each 
block in each rack before loading onto a processor and recording which 
processor that rack went on to. Has anyone got a more efficient way of doing 
this? Assigning at grossing/cut-up is an option, but is an extra step for the 
PA/registrar and relies on knowing which processor is in use that day.

Looking in the crystal ball, will next gen processors automatically scan blocks 
as the first step? Load blocks, start the run and it produces a report of the 
blocks on that run? Interested to hear peoples thoughts.

Cheers,

Chris Hagon - Pathology
Digital Solutions Division, ACT Health Directorate


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Re: [Histonet] Asking for software

[Willis, Donna G via Histonet]

Software comes with the unit when you purchase it from Leica.

Donna Willis
Anatomic Pathology Manager
Baylor University Medical Center
Baylor Scott&White Health
214-820-2465 office

From: Carlos Torres Vega via Histonet 
Sent: Saturday, August 6, 2022 1:36:55 PM
To: histonet@lists.utsouthwestern.edu 
Subject: {EXTERNAL} [Histonet] Asking for software


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Terry Braud
You have used a Leica ISP  slide printer as stand alone unit, what software did 
you use. ?
Because I have one but no software


Enviado desde mi iPhone
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Re: [Histonet] Microwave Reproducibility

[Willis, Donna G via Histonet]

What brand/type of microwave are you using?

Donna Willis
Anatomic Pathology Manager
Baylor Scott&White Health
Baylor University Medical Center
3500 Gaston Ave|Dallas, Texas 75246
214-820-2465 office|214-725-6184 mobile



-Original Message-
From: Lora Jauregui via Histonet  
Sent: Thursday, April 14, 2022 8:26 AM
To: histonet@lists.utsouthwestern.edu
Subject: {EXTERNAL} [Histonet] Microwave Reproducibility


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Any recommendations on a protocol for testing for reproducibility on our 
microwave and documenting it for CAP?  We are a new lab and just setting up 
equipment and such. We only use it to heat water and to heat silver solution 
for our GMS stain.
Thank you

Sent from 
Mail for Windows

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[Histonet] Freida Carson memorial service

[Willis, Donna G via Histonet]

Good Morning,
For those on Histonet that do not read the messages daily, you may not be aware 
of the passing of Freida Carson, PhD.  She was one of our GREAT Educators and a 
friend to many including myself.  Her Celebration of Life Memorial Service will 
be held Thursday January 20, 2022 at 1PM at her church.

Reverend Brent McGuire
Our Redeemer Lutheran Church
7611 Park Lane
Dallas, TX 75225
214.368.1371
revmcgu...@orlcs.com

I know that there are thousands that will not be able to attend the event so I 
have some good news.  The church will be livestreaming the service through the 
church’s YouTube channel for those that would like to view the service remotely 
  
Here
  is the link (Right click and Open Hyperlink)  Also, there will be a light 
reception set up about thirty minutes before the service.  The church wanted it 
to be convenient both to those who would prefer to arrive early and eat 
something before the service and to those who will stay afterwards.

Have a Blessed Day,

Donna Willis
Anatomic Pathology Manager
Baylor Scott&White Health
Baylor University Medical Center
3500 Gaston Ave|Dallas, Texas 75246
214-820-2465 office|214-725-6184 mobile





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[Histonet] Dr Freida Carson

[Willis, Donna G via Histonet]

For those of you that are not on Facebook you may not already know that 
yesterday Freida Carson, PhD became one of our Histology Angels.  I had the 
privilege of having her as my Histology Educator long before she wrote her 
first edition of Histotechnology.  But more precious to me than having her as 
my educator was that she was a friend.  She will be missed.  Thank you Freida 
for all that you have given to the Histology Profession and to me.  Rest In 
Peace singing in the Angels Choir.

Donna Willis
Anatomic Pathology Manager
Baylor Scott&White Health
Baylor University Medical Center
3500 Gaston Ave|Dallas, Texas 75246
214-820-2465 office|214-725-6184 mobile



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[Histonet] Dr. Freida Carson Histology Lab

[Willis, Donna G via Histonet]

Good Morning Everyone,
On December 15, 2021 Baylor Scott&White Health Baylor University Medical Center 
will be naming the Anatomic Pathology Department after Dr. Freida Carson. She 
was a founding member of NSH and served as President and Speaker of the HOD for 
many years. At Baylor she was the department leadership for 35 years, opening 
one of the first NAACLS approved schools in the nation in 1971. Numerous 
Histotechnician have had the privilege to have been trained by her or have used 
one of the 5 editions of her Histology training book throughout the years. 
Naming the lab after her is a little thing compared to what she has done for 
our profession. I am excited that the Baylor Board of Trustees is honoring her 
with this prestigious tribute.


Donna Willis
Anatomic Pathology Manager
Baylor Scott&White Health
Baylor University Medical Center
3500 Gaston Ave|Dallas, Texas 75246
214-820-2465 office|214-725-6184 mobile



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[Histonet] Beaker Tracking vs Vantage

[Willis, Donna G via Histonet]

Good Morning everyone,
I would like to know how many of you are using Beaker Tracking from Epic.  We 
are moving from SoftPathDX to Beaker.  Currently we have Vantage totally 
bi-directional with SoftPathDX for our Tracking/Patient Safety system.  Would 
love to hear from Beaker users about the Beaker Tracking System.  If there is 
someone that had Vantage and went to Beaker can you give detail about the 
differences between the 2 and your preference.  Any helpful hints for a Beaker 
Build.

Thanks and have a Blessed Day,
Donna Willis
Anatomic Pathology Manager
Baylor Scott&White Health
Baylor University Medical Center
3500 Gaston Ave|Dallas, Texas 75246
214-820-2465 office|214-725-6184 mobile



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Re: [Histonet] FREEZING CHAMBER

[Willis, Donna G via Histonet]

We use the PrestoChill from Milestone.

Donna Willis
Anatomic Pathology Manager
Baylor University Medical Center
Baylor Scott&White Health
214-820-2465 office

From: Hannen, Valerie via Histonet 
Sent: Wednesday, August 12, 2020 9:54:37 AM
To: Histonet@lists.utsouthwestern.edu 
Subject: {EXTERNAL} [Histonet] FREEZING CHAMBER


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Good morning all,

I am hoping you can help me.  We are looking for a "freezing chamber/bath" for 
freezing blocks for Frozen Sections.  I have looked and can not find what I am 
looking for. What is everyone else using?  We currently are using a 
"Thermoflask" with 2 Methlybutane and dry ice... this is not working so 
well...the chamber itself is too narrow.

Any help would be greatly appreciated!!

Thanks and have a great day!!


Valerie Hannen,MLT(ASCP),HTL,SU (FL)
Section Chief, Histology
Parrish Medical Center
951 N. Washington Ave.
Titusville,Florida 32796
T: (321)268-6333 ext. 7506
F: (321) 268-6149
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Re: [Histonet] stored in 80% isopropanol

[Willis, Donna G via Histonet]

80% Isopropanol has 20% water. You will still have water in the tissue.

Donna Willis
Anatomic Pathology Manager
Baylor University Medical Center
Baylor Scott&White Health
214-820-2465 office

From: Betsy Molinari via Histonet 
Sent: Wednesday, June 3, 2020 1:39:37 PM
To: 'Histonet@lists.utsouthwestern.edu' 
Subject: {EXTERNAL} [Histonet] stored in 80% isopropanol


CAUTION:  This email originated outside of BSWH; avoid action unless you know 
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Hi,
If tissue is fixed in formalin ( I do not know if it is 10% NBF) then put in 80 
% isopropanol  can the tissue be processed as if it was in ETOH? What are the 
effects to the tissue stored in isopropanol?
Thanks,
Betsy Molinari HT (ASCP)
Texas Heart Institute
Cardiovascular Pathology
6770 Bertner Ave
Houston, TX 77030

832-355-6524 (lab)
832-355-6812 (fax)

Betsy Molinari, HT (ASCP)
Sr. Histology Research Technician
CV Pathology Research

Texas Heart Institute
6770 Bertner Avenue, MC 1-283
Houston, TX 77030

Office: 832-355-6524 | Fax: 832-355-6812
Email: bmolin...@texasheart.org
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Re: [Histonet] Histotechnician open positions

[Willis, Donna G. via Histonet]

Good Afternoon Histology world,
Baylor Scott and White, Baylor University Medical Center Dallas has 2 open 
position on the evening/night shift, in the routine Histology laboratory.  1 
position is full time from 6pm to 2:30am and the other is a part time position 
from 2am to 6am.  Application to these position can be placed on the BSWHealth 
website.

Donna Willis, HT/HTL(ASCP)
Anatomic Pathology Manager
Baylor University Medical Center
3500 Gaston Ave|Dallas, Texas  75246
214-820-2465 office|214-725-6184 mobile
BaylorScottandWhite.com

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the intended recipient (or have received this e-mail in error) please notify 
the sender immediately and destroy this e-mail. Any unauthorized copying, 
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Re: [Histonet] Personel

[Willis, Donna G. via Histonet]

My interpretation of this is that for AP the "test" is the reporting of the 
results.  What is done in the Histology lab to prepare the slides is the 
pre-analytical portion of the test.  

For the revised ANP. 21395 the pathologist is mentioning in the report the 
results of the controls therefore it is in concurrent with the report.  We 
still check our results before taking the slides to the pathologist.

Donna Willis, HT/HTL(ASCP)
Anatomic Pathology Manager

Baylor University Medical Center
3500 Gaston Ave|Dallas, Texas  75246
214-820-2465 office|214-725-6184 mobile
BaylorScottandWhite.com

-Original Message-
From: Jesus Ellin via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Monday, November 28, 2016 8:04 AM
To: Morken, Timothy; Jennifer Valentine-Williams
Cc: Histonet
Subject: [EXTERNAL] Re: [Histonet] Personel

I hope that everyone had a great weekend and a good thanksgiving,, 

Just to shed some light on the subject on the matter to have things in 
perspective,  when I called CAP and here is what the Rep shared with me,  it 
wasn't the interpretation  or the result but rather the QA/QC of the result.  
See below:  now here is the rub on this, as a person doing Special Stains, IHC, 
Digital Path and ISH,,  we as TECH's QA/QC before we hand into the Pathologist, 
our controls are supposed to be reviewed by the tech to make sure the reaction 
took  place, we don't say it looks brown, or red, or hey the colorful,, WE 
confirm the reaction, cause if not we redo.  This is what ALSO the CAP states 
that we do because it does say Pathologist or Designee , and it's the QA/QC 
step.  By no means are we resulting or giving Diagnosis/interpretation.  This 
is where I am having trouble with this statement, about 
testing/interpretation,, it has nothing to do with that at all.  CLIA also 
doesnt define QA/QC , just resulting/interpretation.

They are the same Steps that are being followed on digital path, were imaging 
of the slides require a person of High Complexity testing to be doing the 
scanning.  Again this is another QA/QC step, so I get all the stuff about CLIA 
and reporting, but CAP is specific and we are doing the QA/QC of this testing.

I also had a conversation about whether Licensure was acceptable or not,,  
without meeting the requirements, and I was told NO,, they need to meet the 
minimum requirements as stated in CLIA for high complexity testing,, again 
something to look at.

I then called ASCP to ask them about where they stood with this and was told we 
only do the licensure not the regs.

I called CMS to get a better understanding stating the issue, as for them the 
person interpreting and reporting is the Pathologist, but if CAP requires that 
the QA/QC be done with those regs,, then that is more than is required, but 
that is their regulation and to be adhered too.

Funny thing then when you go the CLIA website you get the high complexity 
testing, you can see where the testing methodologies are and can see when the 
test system came about. 

I know this is going to be a debatable situation and I have to agree with Tim,, 
please standardize and let us know,, but it is clear what they are saying about 
results, testing , interpretation and now QA/QC..  

Thoughts anyone,, but I see that this is going to change histology as a whole, 
since now this is being added too the mix.  I am not saying it is right or 
wrong,, but it does put forth effort that we need to classify what we do and 
also try to align the test system accordingly, because QA/QC is almost 
everything,, you can put this on instrumentation, protocols, procedures, etc.  

Also what do we do with those that have been doing this for years,, that have 
the knowledge and also the background,, again I don't wish to open up PANDORA's 
box,, I also know we are all going to look at this differently,, but the facts 
are we are changing and if we do not own the processes we currently do, someone 
else will that's for sure.

Jesus Ellin



**REVISED** 08/17/2016
ANP.21395 Special Stains/Studies Phase II For special stains, including 
histochemical stains, and studies using immunologic and ISH methodology, 
positive and negative controls are verified and recorded as acceptable prior to 
or concurrent with the reporting of patient results and records maintained.

NOTE: Controls must be verified and recorded as acceptable by a pathologist or 
designee (provided the designee meets high complexity testing qualifications).

Positive tissue controls must contain the component specific to the special 
stain that is being applied to the specimen.
Immunohistochemical tests using polymer-based detection systems (biotin-free) 
are sufficiently free of background reactivity to obviate the need for a 
negative reagent control and such controls may be omitted at the discretion of 
the laboratory director following appropriate validation.
If interpretation of the special stain or study is performed by a different 
laboratory, there

[Histonet] Baylor Dallas Recuritment Event

[Willis, Donna G. via Histonet]

Baylor Scott&White North Texas will be hosting a Recruitment Event on August 
27, 2016 from 10AM to 1PM.  The Link to the Event flyer is below.

http://bit.ly/BSWHLab_Rad_August2016

Thanks,

Donna Willis, HT/HTL(ASCP)
Anatomic Pathology Manager

Baylor University Medical Center
3500 Gaston Ave|Dallas, Texas  75246
214-820-2465 office|214-725-6184 mobile
BaylorScottandWhite.com

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[Histonet] Baylor University Medical Center Histology Supervisor

[Willis, Donna G. via Histonet]

Good afternoon everyone.  I have a new position that I would like to mention.  
Hope to get some good applicants.

Supervisor, Histology - Dallas, Texas - Baylor Scott and White Health
Requisition Number: 193596_1

The Histology Lab Supervisor oversees/supervises the routine activities of the 
laboratory or laboratory section to ensure that staffing is adequate and that 
tests are performed, recorded, and reported in a timely manner and in 
accordance with quality standards. The Histology lab Supervisor assists the 
manager as defined by the laboratory or laboratory section policy, with staff 
hiring, training, performance evaluations, payroll, etc.

Requirements:
HT or HTL (License/Certification) Required
2 Years of Experience Associated with Progressive Responsibility
College Degree- Bachelor of Science

If you are qualified and interested in applying to this position, please go to: 
http://jobs.baylorscottandwhite.com/ and enter the job requisition number 
193596_1 in the keyword search.

Thanks to all,

Donna Willis, HT/HTL(ASCP)
Anatomic Pathology Manager
Baylor University Medical Center
3500 Gaston Ave|Dallas, Texas  75246
214-820-2465 office|214-725-6184 mobile
BaylorScottandWhite.com


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