[Histonet] Suggestions for staining ground substances in the Heart
Hi all, We are interested in staining ground substances in the heart. Are there other stains that will work beside the Pentachrome? We have tried the Movat's pentachrome (commercial kit) and are able to demonstrate ground substance in the bone with the alcian blue part of this stain. In our hands the goblet cells of the gut and the ground substance in the heart are devoid of staining. We have tried sections cut at 4 and 6 microns. Any thoughts/suggestions? Kristie Christina Wolfe, BSHA, MLT (ASCP), HT, QIHC Drug Safety Evaluation/Bristol-Myers Squibb Pathology Dept. 812-307-2093 This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Movat's Pentachrome Paper?
Hi all, Does anyone have a pdf of Movat's paper Demonstration of All Connective Tissue Elements in a Single Section. Arch. Path. 60: pp 289-295 Thanks! Kristie Christina Wolfe, BSHA, MLT (ASCP), HT, QIHC Drug Safety Evaluation/Bristol-Myers Squibb Pathology Dept. 812-307-2093 This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Bubble problems
Hi Melissa, We have intermittently had problems with our Leica Glass coverslipper over the years. During troubleshooting with their tech support, we found our issue was most likely with the coverslips. We use the VWR micro glass 24x50 mm cat# 48404-452 coverslips and the Tissue-Tek Glas mounting medium cat #6419. There are a couple of settings on the instrument that control the amount of mounting medium flow onto the glass slide - you can call me if you want our settings that work well for us. Also, the xylene in the 'holding area' on the coverslipper should be changed regularly and needs to be pretty full (there is a line on ours). We have found if that xylene is too old and if the volume of xylene drops below the fill line - we start getting micro bubbles. One last thing, it is important to also push the button to drop the mounting medium dispenser at the end of the day to be submerged in the little container of xylene and to make sure and clean the rubber stopper that picks up the glass coverslips with the brush before each day of use. We have found that all of these elements affect air bubbles on the slides. Feel free to give me a call if you like. Christina Wolfe Bristol Myers Squibb 812-307-2093 From: histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu on behalf of Melissa Ng melissa...@spdscientific.com Sent: Thursday, August 07, 2014 7:59 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Bubble problems! Dear Histonet, I am having a major problem with bubbles in my mounting media. I am currently using the Leica coverslipper and Cellpath mounting media (Phalate free). Micro-bubbles are observed on the slides after sometime of coverslipping. In fact, when I checked the media, I found lots of bubbles inside the bottle too! There were no bubbles in the bottle prior to placing the mounting media with the machine (I checked!). Also, we do not see any bubbles in the tubes that lead to the probe! Previously, we were using the Leica mounting media (yellowish liquid) and there were no bubble issues. I have checked with other labs using the Cellpath mounting media (they use Leica coverslipper too) and no one seems to be having the same problem as us! The Cellpath mounting media is way more viscous compared to that of the Leica media. Has anyone here experienced a similar issue, and how have you resolved it? Appreciate all the help I can get! Best regards, Melissa Application Specialist ___ ** This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Help Please - Need a Leica Rep to contact me
I am in Indiana - I need a Leica sales rep to contact me please or if someone can provide me with a regional sales rep name - that would be awesome! Thanks! Kristie Ph 812-307-2093 christina.wo...@bms.com This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 128, Issue 6 - Leica Rep Question
Hello all, Thanks for the prompt response to my inquiry to find an equipment sales rep! Leica has very quickly been attentive to help me out. Great job Mike Dave - thanks again! Kristie Message: 7 Date: Tue, 8 Jul 2014 12:58:15 -0400 From: Wolfe, Christina christina.wo...@bms.com Subject: [Histonet] Help Please - Need a Leica Rep to contact me I am in Indiana - I need a Leica sales rep to contact me please or if someone can provide me with a regional sales rep name - that would be awesome! Thanks! Kristie Ph 812-307-2093 christina.wo...@bms.com This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 128, Issue 6 This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Lendrum's stain for Paneth Cells
Hi, Does anyone have a PMID for Lendrum's original paper LENDRUM, A.C. 1947. The Phloxine-tartrazine method as a general histological stain and for the demonstration of inclusion bodies. Journal of Pathology and Bacteriology.? I have searched PubMed and I am not getting any results. We are having trouble getting this stain to work in our lab. The Paneth cells are not staining and there is no yellow stain at all with the tartrazine. We are trying to stain rat ileum. Anyone with experience using this stain - please help. :) Thanks! Kristie Christina Wolfe, BS, MLT (ASCP), HT, QIHC Drug Safety Evaluation/Bristol-Myers Squibb Pathology Dept. 812-307-2093 This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: anyone out there doing a DMAB-nitrite stain for tryptophan?
As a follow up to my original questions - I now understand the ceolloidinise is the procedure using celloidin. I am still having trouble finding a vendor for celloidin. Can I substitute LVN in place of the celloidin? Or maybe there is another method to use in this step. Any feedback will be greatly appreciated! Kristie Christina Wolfe, BS, MLT (ASCP), HT, QIHC Drug Safety Evaluation/Bristol-Myers Squibb Pathology Dept. 812-307-2093 From: Wolfe, Christina Sent: Monday, May 12, 2014 2:25 PM To: 'histonet@lists.utsouthwestern.edu' Subject: anyone out there doing a DMAB-nitrite stain for tryptophan? Hi, We are wanting to do the DMAB-nitrite method for tryptophan (Bancroft is the reference). I have some questions about the method. If anyone has experience, please contact me. Below are my questions: 1.Take sections to alcohol. (What percentage? Not sure if this matters). 2. Celloidinise in 0.5 per cent celloidin.(Where can I find celloidinise - is this still sold commercially?) 3. In step 6 of the procedure there is an acid alcohol rinse (anyone know what the acid alcohol concentration should be?) Thanks in advance if you can help! Kristie Christina Wolfe, BS, MLT (ASCP), HT, QIHC Drug Safety Evaluation/Bristol-Myers Squibb Pathology Dept. 812-307-2093 This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
