Re: [Histonet] Procedure for Blue Cheese for Fungus Controls

[cynthia haynes via Histonet]

I would like a copy of your procedure please. My email addresses is 
naje1...@yahoo.com. Thank you 

Sent from Yahoo Mail on Android 
 
  On Sat, Nov 23, 2019 at 4:14 PM, John Garratt via 
Histonet wrote:   If you have access to 
spare fresh tissue (ie placenta) you can infuse the tissue with fungus (blue 
cheese if you wish) and cultivate overnight before FFPE, I have found these 
make excellent control blocks and the tissue makes for good structural support, 
plus the tissue architecture might make the blue cheese more palatable to 
pathologists. Your idea is certainly not crackers.

John


www.ciqc.ca

‐‐‐ Original Message ‐‐‐
On Friday, November 22, 2019 5:01 PM, Akemi via Histonet 
 wrote:

> Hi Histopeeps! Here’s my procedure for Fungus Test Controls since we are out 
> of controls. This was from Blue Cheese I processed yesterday. It turned out 
> fabulous!!! Photo credit to Christina George (i know Histonet doesn’t attach 
> photos so can send to you) and lab credit to my staff, Quinnlan Dewitt and 
> Carlos Duran. I used President brand Le Blue which I purchased from Sprouts 
> grocery. It cost $7.95 for 3.5 oz. it was the most aged. I then took a 
> portion of the “moldy blue” cheese and put it in lens paper, then cassetted 
> it and fixed it in 10% Formalin in the morning for 8 hours and ran it 
> normally with routine surgicals. Thinking of publishing!
>
> Cheers!
>
> Sent from my iPhone
>
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet



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Re: [Histonet] Dermatopathology Histology Opportunity Full Time

[cynthia haynes via Histonet]

Sent from Yahoo Mail on Android 
 
  On Mon, Jul 15, 2019 at 11:56 AM, Pam Barker via 
Histonet wrote:   Hi Histopeeps!
Happy Monday!
I have been engaged exclusively by a private Dermatopathology lab to find a
fantastic histotech to work as the sole practioner in this state of the art
lab.
This is a full time permanent M-F day shift position and my client offers a
great pay rate, nice benefits and an excellent environment to work in.
For more information please contact me – Pam Barker at rel...@earthlink.net
or asap on my cell/text at 407-353-5070 or toll free at the office at
866-607-3542.
If you refer someone to me and I place them you will earn a referral fee!  
Have a great day!

Thanks-Pam

#jobs4myhistopeeps 
#ilovemyhistopeeps
#histopeeps
Follow my hashtags and make your day great and your career greater!!

Right Place, Right Time, Right Move with RELIA!

Thank You!
 Pam M. Barker 
Pam Barker
President/Senior Recruiting Specialist-Histology
RELIA Solutions
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
E-mail: rel...@earthlink.net 
https://www.facebook.com/RELIASolutionsforhistologyprofessionals
www.facebook.com/PamBarkerRELIA
www.linkedin.com/in/reliasolutions
www.twitter.com/pamatrelia 







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[Histonet] Inventory for sales

[cynthia haynes via Histonet]

Hello all My lab closed in February of this year. Please email me for a list of 
supplies we have for sale. Thank you Cynthia James H.T.

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Re: [Histonet] SV40 control tissue/slides

[cynthia haynes via Histonet]

Hello KachemakPleasecontact me at naje1...@yahoo.com. I think I can help you 
with obtaining this control tissue. Cynthia James H.T 
Sent from Yahoo Mail on Android 
 
  On Tue, Nov 28, 2017 at 8:16 AM, kalkhenaizi . via 
Histonet wrote:   Hello histonetters,

Can someone advise where I can get SV40 control tissue/slides?

Thanks,

Kadhem
ExpressMed Labs
Bahrain
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Re: [Histonet] Histonet Digest, Vol 164, Issue 5

[cynthia haynes via Histonet]

Are you batch staining or you placing a control on every slide; if batches are 
used  a good controld block will last  6 month or more. Placing a control on 
every slide needing a stain will last 3 month. I will try to get you a block 
free of charge. Cynthia James H.T 

Sent from Yahoo Mail on Android 
 
  On Fri, Jul 14, 2017 at 12:55 PM, Brent Adams via 
Histonet wrote:   
Hey folks,



I am looking to purchase some blocks with positive H pylori tissue.

I have never done this before as we always had plenty of positive tissue in the 
wet tissue room. Has anyone had to buy tissue?

If you have, who did you find to be good to work with and how is

it sold, by the block by the case by the pound ( LOL). Really could use some 
direction on this. I just cant keep enough acceptable positive H pylori control 
slides on hand and am always running out. Would like to have a 6 month supply 
to work from and be able to keep a head with.



Thanks


Brent Adams – BS, LPN, HT





www.acadianagastro.com



Acadiana Gastroenterology Associates, LLC

439 Heymann Blvd

Lafayette, LA  70503



tel:  (337) 269-1126

fax:  (337) 269-1476


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Today's Topics:

  1. Pathology Band Saw (Sandra Cheasty)


--

Message: 1
Date: Fri, 07 Jul 2017 19:33:36 +
From: Sandra Cheasty 
To: "Histonet (histonet@lists.utsouthwestern.edu)"
        
Subject: [Histonet] Pathology Band Saw
Message-ID:
        


Content-Type: text/plain; CHARSET=US-ASCII

Hello,
                Can anyone recommend a band saw for pathology? We are looking 
for a new band saw for use in necropsy and research. It has to be a "meat" band 
saw, as workshop band saws aren't compatible with wet tissue.
A table-top model is preferred, and cheaper than a floor model. (Plus, the 
floor models all seem to come with a sausage-maker, which is pretty gross.)
                If you have used a table-top band saw in your pathology lab, 
I'd appreciate hearing from you.
Cheers!
Sandy

Sandra J. Cheasty, HT (ASCP)
Histology & Necropsy Supervisor
UW-Madison, School of Veterinary Medicine



--

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Re: [Histonet] TTG

[cynthia haynes via Histonet]

My pathologist usually asks for CD68 to be done on possible celiac 
cases.Cynthia Haynes-James H.T 
Sent from Yahoo Mail on Android 
 
  On Fri, May 26, 2017 at 12:35 PM, Pairan, Kelly via 
Histonet wrote:   Happy Friday!

A special thank you to everyone who answered by question about Inhibin 
yesterday.  One of my pathologists has now asked me to reach out to all of you 
to ask if anyone is using TTG for Duodenal Biopsies from possible Celiac 
patients.  If so, where are you currently getting your antibody and do you have 
any problems with the staining?

Thank you for your help and have a lovely long weekend!
Kelly
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Re: [Histonet] A Question About Paraffin Times

[cynthia haynes via Histonet]

Please don't leave the tissue in the hot wax.They will harden and be brittle. 
Cynthia James H.T 

Sent from Yahoo Mail on Android 
 
  On Tue, May 16, 2017 at 10:50 AM, P Sicurello via 
Histonet wrote:   Good Morning Listers,

I am asking the collective wisdom of the Histonet this question:


Is it better to remove baskets from the processor on Saturday morning and:

A.  Let the cassettes freeze, then melt them down and embed Monday morning?
OR
B.  Leave the cassettes in molten paraffin and embed Monday morning?


I am of the opinion that leaving the samples (not fatty, like breast cores)
in molten paraffin (62 degrees C)  is bad practice, and causes them to get
"crunchy", among other things.

What do you think?

Thank in advance.

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

200 Arbor Drive

San Diego, CA 92103

(P): 619-543-2872 <#>



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[Histonet] Part time /As needed position

[cynthia haynes via Histonet]

I am in need of someone to provide coverage for  sick or vacation days. This 
person will also be offered weekends as well. It would be every other weekend. 
I am located in Chicago, Illinois. I can be reached at naje1...@yahoo.com or 
312-919-9887.Thank you in advancedCynthia HJames H.T.
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[Histonet] HMGCoA Reductase analysis

[cynthia haynes via Histonet]

I have a researcher in need of a lab that is doing HMGCoA Reductase procedures, 
if you are doing this type of staining; please contact me at naje1...@yahoo.com 
or 312-919-9887.Thank youCynthia Haynes-James H.T.
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[Histonet] Wax clogs in VIP 6 processor

[cynthia haynes via Histonet]

 Hello Histo NetI am having a problem with wax clogs on my VIP 6 processor. 
This unit vents to the outside and I was just wondering if that may have 
something to do with my problem. It started 2 weeks ago. I've had the unit 
serviced 2 x's. It has been very humid in our city. I am changing sol'n 
regularly as per company supplied maintenance program. HELP!. I am at my wits 
end. This relatively a new unit. 3 year old. Has anyone experience this problem 
before.Thanks in advance.Cynthia Haynes-James H.T.
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[Histonet] Labelers for slides and paraffin blocks

[cynthia haynes via Histonet]

Vendors are welcome to contact me at naje1...@yahoo.com. I need pricing 
information as well . Cynthia Haynes-James H.T 

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Re: [Histonet] de-waxing protocol on instruments just fact finding

[cynthia haynes]

Only if you don't de-wax long enough. 

De-wax for 10 minutes  in xylene  at least 3 changes each. Then dehydrate 5 
minutes in 100 %  alcohol 3 changes each. Then 95% alcohol 2 changes each for 5 
minutes. Then 80% 2 minutes each and finally 70% 1 minute,then rinse well in DI 
h2o.

hope this helps. 



Sent from Yahoo Mail on Android

From:Rachel M Gonzalez rac...@gbi-inc.com
Date:Mon, Jun 22, 2015 at 5:12 PM
Subject:[Histonet] de-waxing protocol on instruments just fact finding

Hi

Just beginning to look at instruments DAKO Ventana Leica. We may not have
the budget but if we do However talking to the sales reps have been
challenging as everything is proprietary.

This is for research consideration so we will use an open instrument? Which
instrument do you like better open?

Does  manual dewaxing affect the staining? Every rep says yes... but does
it?

What is the time for dewaxing when you start the instrument? What are the
number of steps in the protocol? What is the waste generation like more or
less than manual?

Thanks for your help.

Rachel
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[Histonet] (no subject)

[cynthia haynes]

My name is Cynthia James; I work in a histopathology lab in Melrose Park 
Illinois. We are told to change the solution(2-Methyl Butane) which we use in 
the Histobath to freeze tissues for frozen sections to something less 
flammable. I remember a solution was discussed that can be used instead of the 
which is less flammable than the  IsoPentane that we are currently using. 
Would someone contact at 708-681-3200 ext:2012 or 2036 and tell me more about 
this solution.I am available from 5:00 am to 1:30 pm monday-friday central 
time, if you know the company that is selling this solution please let me know.
 
Thank you in advance
Cynthia James
 
P.S I can also be reached at this email address naje1...@yahoo.com or 
cynthia_haynes-ja...@luhs.org
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[Histonet] part time histology job in chicago

[cynthia haynes]

We have a part time histology position located at Gottlieb memorial hospital in 
Melrose Park, Illlinois; if anyone is interested in a part time histology 
please give us a call at 708-618-3200 ext 2012.

You can also go to the Gottlieb website and fill out the application and submit 
your resume there as well; if you go that route your application and resume 
will go directly to the lab manager and she will forward it to me. Our fax 
number is 708-681-7874.

Thank you 
Cynthia Haynes-James H.T.





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