Our lab has started using Immunocal decal solution on our bone marrows. Most of
our antibodies have improved except kappa and lambda. We do not do ISH. Kappa
and lambda staining in the tonsil controls is good, The bone marrows are now
very overstrained. We use DAKO polyclonals at 1:10,000 with protease1 on the
Ventana Ultra. Does anyone have a procedure?
- Original Message -
From: histonet-requ...@lists.utsouthwestern.edu
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 124, Issue 24
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Today's Topics:
1. specimen marking ink (Davis, Cassie)
2. Leica Reichert Jung Cryocut 1800 (King, Laurie J)
3. RE: specimen marking ink (wanda.sm...@hcahealthcare.com)
4. IHC on paraffin embedded skin tissue! (Jennifer Leigh)
5. Re: RE: specimen marking ink (David Kemler)
6. Re: RE: specimen marking ink (Bryan Llewellyn)
7. Re: IHC on paraffin embedded skin tissue! (C.D.G.)
--
Message: 1
Date: Fri, 21 Mar 2014 13:03:35 -0400
From: Davis, Cassie cda...@che-east.org
Subject: [Histonet] specimen marking ink
To: histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Message-ID:
be7af02009c3a74f9b8344e70f3655cbfb9...@chexcms01.one.ads.che.org
Content-Type: text/plain; charset=iso-8859-1
Hi Histo World, as I was cutting to day I was thinking why don't we see if we
could get specimen marking ink directly from a tattoo vendor? When I first
started in histo I was told the ink we use was actually tattoo ink. As we know
as soon as somebody labels something as a medical supply the price is
increased. Just a cost saving thought, I mentioned it to my immediate
supervisor but she think it would be a liability issue. I thought we could
test/validate it on skin tissue left over from a mastectomy or extremity. Any
thoughts?
Cassandra Davis
cda...@che-east.org
302-575-8095
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Message: 2
Date: Fri, 21 Mar 2014 17:45:48 +
From: King, Laurie J king.lau...@marshfieldclinic.org
Subject: [Histonet] Leica Reichert Jung Cryocut 1800
To: 'histonet@lists.utsouthwestern.edu'
(histonet@lists.utsouthwestern.edu)
histonet@lists.utsouthwestern.edu
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7578207839f50248a7a6cd33517295ea4f13b...@mcl-exmb03.mfldclin.org
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Hello all,
Looking for a manual for a Leica Reichert Jung Cryocut 1800.
Laurie
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Message: 3
Date: Fri, 21 Mar 2014 14:37:59 -0500
From: wanda.sm...@hcahealthcare.com
Subject: [Histonet] RE: specimen marking ink
To: cda...@che-east.org, histonet@lists.utsouthwestern.edu
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9e2d36ce2d7cba4a94d9b22e8328a3ba27efddf...@nadcwpmsgcms03.hca.corpad.net
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Back in the 70's (when I was six, but still a histotech!!!) my Pathologist had
gotten tattoo pigment powder in two colors and that's what we used to mark
tissue. Don't know where it came from and don't know where it went!!!
Happy Friday and Happy Weekend to everyone!!!
Wanda
WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC� 29406
843-847-4586
843-847-4296 fax
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