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On Jul 22, 2013, at 10:01 AM, histonet-requ...@lists.utsouthwestern.edu wrote:
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Today's Topics:
1. Re: Re: Carnoy solution (Jay Lundgren)
2. Re: Spirochete IHC on H-Pylori infected Tissue ? (Rene J Buesa)
3. RE: Transcriptionist Productivity (Cindy Pyse)
4. Re: Transcriptionist Productivity (Rene J Buesa)
5. Knife sharpener glass plate (Hannen, Valerie)
6. Part-time Histo needed- East Bay of San Fran (Heidi Hawthorne)
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Message: 1
Date: Sun, 21 Jul 2013 14:42:49 -0500
From: Jay Lundgren jaylundg...@gmail.com
Subject: Re: [Histonet] Re: Carnoy solution
To: Bob Richmond rsrichm...@gmail.com
Cc: histonet histonet@lists.utsouthwestern.edu
Message-ID:
CANCZNuZKDCmqdFB1c=mp465orzvh3z4qygfcckph1iya8yb...@mail.gmail.com
Content-Type: text/plain; charset=ISO-8859-1
Whenever I am asked to make Carnoy's I reply, Vroom-vroom!.
Sincerely,
Jay A. Lundgren, M.S., HTL
(ASCP)
On Fri, Jul 19, 2013 at 11:56 AM, Bob Richmond rsrichm...@gmail.com wrote:
Carnoy's fixative contains chloroform, and that's enough reason not to use
it. In addition to its toxicity, chloroform dissolves methacrylate plastic,
as I found out the hard way once when I tried to fix a specimen in Carnoy's
fixative in a methacrylate container because I had nothing else.
I've seen Carnoy's fixative used as a disclosing fixative for finding
mesenteric lymph nodes in colectomy specimens. There's no excuse for this.
There are a number of proprietary fixatives (such as Surgipath's O-Fix) for
this purpose, or if you're allowed to brew your own, I use Davidson's
fixative (3 parts water, 3 parts reagent alcohol, 2 parts 37% formaldehyde
[NOT 10% NBF], 1 part glacial acetic acid.)
Bob Richmond
Samurai Pathologist
Maryville TN
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Message: 2
Date: Sun, 21 Jul 2013 13:11:34 -0700 (PDT)
From: Rene J Buesa rjbu...@yahoo.com
Subject: Re: [Histonet] Spirochete IHC on H-Pylori infected Tissue ?
To: a b zipper...@gmail.com,histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Message-ID:
1374437494.60048.yahoomail...@web163106.mail.bf1.yahoo.com
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You are overlooking the essential characteristic of IHC, namely, specificity.
Ab to H-Pilory will react only with H-Pilory, and the same goes for
Spirochete.
You will be wasting all your reagents and, worse of everything, your
supervisor will wonder about your knowledge.
René J.
From: a b zipper...@gmail.com
To: histonet@lists.utsouthwestern.edu
Sent: Saturday, July 20, 2013 10:09 PM
Subject: [Histonet] Spirochete IHC on H-Pylori infected Tissue ?
So here I am working my Saturday night, running an Immuno for Monday, only
to open the fridge and find no more H-Pylori antibody cause we're out! : (
Can I substitute with Spirochete Antigen, and If so will the result will be
as strong?
As a standard procedure, I am doing a Warthin Starry on it also,
Advice would be great. Thanks
Belma Woodcraft ( Histology Lead )
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Message: 3
Date: Mon, 22 Jul 2013 08:26:12 -0400
From: Cindy Pyse cp...@x-celllab.com
Subject: RE: [Histonet] Transcriptionist Productivity
To: 'Willis, Donna G.' donna.wil...@baylorhealth.edu,
histonet@lists.utsouthwestern.edu
Message-ID: 000f01ce86d6$a720a1a0$f561e4e0$@x-celllab.com
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I also would be interested in any replies.
Cindy
Cindy Pyse CLT, HT(ASCP)
Laboratory Manager
X-Cell Laboratories of WNY
20 Northpointe Parkway Ste 100
Amherst, NY 14228
716-250-9235 Ext. 232
cp...@x-celllab.com
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Willis,
Donna G.
Sent: Friday, July 19, 2013 1:10 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Transcriptionist Productivity
For Managers of Anatomic
