Re: [Histonet] RE: sections not sticking to charged slides
We follow the same process as Jeff. Michelle On Feb 24, 2015, at 4:59 PM, Jeffrey Robinson jrobin...@pathology-associates.com wrote: HI Renee- I had a major problem in the past with tissue staining for IHC lifting off the slides no matter what I tried. It turned out that when we precut our controls and put them in the oven and then used them later for the patient tissue that the charge on the slide was altered. We currently still precut controls on control slides (we use Leica APEX) and then let them just air dry with no heat. We then use those slides when needed and add the patient tissue to the bottom and then put the slides in the oven (we use 70C for one hour) prior to IHC staining. The tissue lifting decreased dramatically- even on breast tissue. Be sure to pick up your controls from the wrong (label) end when picking up your controls and do not let the bottom half of the slide get into the waterbath so as to avoid double-dipping which can also cause tissue adherence problems. Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Renee H. Workman Sent: Tuesday, February 24, 2015 1:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] sections not sticking to charged slides Help, sections not sticking to charged slides. We use Mercedes Medical charged slides. Lately have been using sta-on but still have occasional problems especially during antigen retrieval. I need any suggestions. Renee H. Workman Histology Supervisor Virginia Urology 9105 Stony Point Drive Richmond, VA 23235 W: 804-527-1316 | F: 804-270-0917 rhwork...@uro.com | www.uro.com Disclaimer: The email and files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the original recipient or the person responsible for the delivering the email to the intended recipient, be advised that you have received this email in error, and that any use, dissemination, forwarding, printing or copying of this email is strictly prohibited. If you received this email in error, please delete it from your system without copying it, and notify the sender by reply email so that our address record can be corrected. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Requirements that tissue cassette storage cabinets be off floor
I agree with Sheila. At our place, it was fire code that everything be off the ground by 6... not just blocks, but anything being stored. Michelle Sent from my iPhone On Feb 9, 2015, at 1:30 PM, Tapper, Sheila J. sheila.tap...@essentiahealth.org wrote: In my experience, this is usually a local fire code requirement. Sheila -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Debra Siena Sent: Monday, February 09, 2015 11:35 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Requirements that tissue cassette storage cabinets be off floor Hi Histonetters- Can anyone tell me if it is a JCAHO, CAP or CLIA requirement that tissue cassette storage cabinets be at least 6 inches off the floor? Also, If possible, could you tell me how I could find this informaiton? Any information is appreciated, thanks for your help in advance. Debbie Siena, HT(ASCP)QIHC StatLab Medical Products Technical Support Manager 407 Interchange Street | McKinney, TX 75071 t: 800.442.3573 ext. 229 | f: 972.767.3992 dsi...@statlab.com mailto:bbro...@statlab.com%7C | www.statlab.comhttp://www.statlab.com/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Frozen Sections
We provide assistance, primarily on the outpatient frozens, but help with in-patient ones as well. What makes you ask? Michelle Sent from my iPhone On Feb 6, 2015, at 10:37 PM, lsmal...@juno.com lsmal...@juno.com wrote: Hi, I need to ask Histoland a questionHow many HT departments provide assistance to the pathologist in the performance of frozen sections (cutting and staining of slides) to be evaluated by the pathologist? Thank you very much in advance! Lorraine Fast, Secure, NetZero 4G Mobile Broadband. Try it. http://www.netzero.net/?refcd=NZINTISP0512T4GOUT2 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Re: Embedding
We also use the para-trimmer. In my view, it is worth its weight in gold! I can melt 5 blocks at a time, works like a charm. I am one who does not mind the wax on the sides, as I am most confident that there is enough paraffin to support the cassette. Michelle Sent from my iPhone On Jan 21, 2015, at 11:41 AM, Morken, Timothy timothy.mor...@ucsf.edu wrote: I agree. We got one of these a couple years ago and the techs love it. It is a heated block on which you rub the cassette. The paraffin melts away. It is especially good for preserving barcodes (but don't press the printed surface on the heat block too long - you can soften the print and cause some damage, but nothing like can happen with scraping). Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa Sent: Wednesday, January 21, 2015 8:29 AM To: gayle.cal...@bresnan.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Embedding I agree with Gayle. We finally purchased a trimmer from Ted Pella - lowest price by far - and are saving our finger joints. The amount of wax remaining on the cassette also appears to depend on the brand of mold used. Tresa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Wednesday, January 21, 2015 9:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Embedding After years of never winning the battle of paraffin on cassette edges after embedding, we purchased a paraffin block trimmer. It saves time and the stress on finger joints compared to scraping cassettes daily. No matter how careful we were during embedding to keep excess paraffin off cassette edges, we were never successful. Several vendors have these and you may be able to find a refurbished one. Gayle M. Callis HTL/HT/MT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tesr
Please disregard Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Automatic H E slide stainer recommendations
I second the Leica XL. That stainer is a reliable workhorse. Sent from my iPhone On Jan 7, 2015, at 8:08 PM, ian bernard ian.bern...@comcast.net wrote: The Leica Autostainer XL has proven effective for our lab. IRB -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Conway, Carla Sent: Tuesday, January 06, 2015 2:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automatic H E slide stainer recommendations Hello colleagues, I would appreciate any recommendations for an automatic slide stainer. It will primarily be used for H E staining, not IHC. Thanks in advance, Carla Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street Seattle, WA 98115-5016 USA Phone: 206-526-2042 Fax: 206-526-6654 E-mail: cmcon...@usgs.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] New 88344 CPT code question
Hello Histonetters :) With regards to billing for multiplex IHC stains, I have a scenario and question. Scenario: 1 container, 2 blocks, 2 different cocktail IHC stains. Stain1 is done on both blocks A1 and A2. Stain2 is done on block A1. How would we bill for this? Would it be 88344X2 - because there are two distinct cocktails. OR would it be 88344X1 - because you can only charge one per container? Thanks in advance, Michelle Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Facility Name on Slides
Mary, thank you for your reply. Can you point me to a specific regulation from which I can quote? Thanks! Michelle On Oct 15, 2012, at 9:36 AM, MARY T HODGES hodges...@msn.com wrote: The nuber and name of patient as well as the hospital is required Sent from my Verizon Wireless BlackBerry -Original Message- From: Jesus Ellin jel...@yumaregional.org Date: Mon, 8 Oct 2012 23:40:37 To: histot...@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Facility Name on Slides To my knowledge there is no regulation rather than having 2 identifiers,, Sent from my iPad On Oct 8, 2012, at 1:46 PM, histot...@imagesbyhopper.com histot...@imagesbyhopper.com wrote: Does anyone have any regs that you can point me to for both Joint Commission and CAP which details definitively whether or not a facility name is *required* to be included on slides? I know it's a good idea, the larger question here is whether or not there is a specific regulation for it? Thanks for all your help! Michelle ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Reprocess Procedure
Once the block has been faced, we melt the excess paraffin from around the tissue, being careful not to seal the exposed tissue in paraffin. We remove the tissue from it's embedded cassette, put it in a newly numbered cassette and place it in the processor for reprocessing. Works well in our lab! On Oct 15, 2012, at 3:41 PM, kira...@sbcglobal.net wrote: Thank you for your input. I would like to know how others are handling reprocess. Kiran Sent from my Verizon Wireless BlackBerry -Original Message- From: Grantham, Andrea L - (algranth) algra...@email.arizona.edu Sender: histonet-boun...@lists.utsouthwestern.edu Date: Mon, 15 Oct 2012 15:28:21 Cc: histonet@lists.utsouthwestern.eduhistonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Reprocess Procedure A few years ago this subject was discussed on histonet and somebody - I think it was Joe the Toe - said that they just melted the paraffin away from the tissue if it had been embedded and threw it back in to process with the next batch of tissues. The theory being that the part of the tissue that was processed ok was protected by the paraffin and the part that needed additional processing was exposed. I don't have to reprocess often but when I was gone a student had a problem with the processor and put in an emergency call to me. I told her to do this and the tissues came out fine the next day. Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edumailto:algra...@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Facility Name on Slides
Does anyone have any regs that you can point me to for both Joint Commission and CAP which details definitively whether or not a facility name is *required* to be included on slides? I know it's a good idea, the larger question here is whether or not there is a specific regulation for it? Thanks for all your help! Michelle ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: back up cryostat
We have two back ups! :) We also have back up tissue processors and microtomes as well. Sent from my iPhone On Sep 21, 2012, at 8:15 AM, Manfre, Philip philip_man...@merck.com wrote: If you use anything often enough, it is wise to have a back-up, if possible. Microtomes, and especially cryostats, tend to malfunction without warning and can leave you stranded. We have a back-up that isn't ours, but we have access to it. That's another option. Make friends with someone else who has one and strike an arrangement with them. Philip Manfre, BA, HT(ASCP) Associate Principal Scientist Merck Research Laboratories WP45-251 PO Box 4 West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_man...@merck.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of barbara.cr...@lpnt.net Sent: Friday, September 21, 2012 8:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] back up cryostat I would like to know what the consensus is on having a backup cryostat. Does anyone have a back up cryostat? I am nervous about not having a backup cryostat Antoinette Crill TEAM LEADER ANATOMIC PATHOLOGY X5451 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Milestone Histos 5
Does anyone know of a company who buys used Histology equipment and would give quotes for used Milestone Histos 5 microwave tissue processors. Thanks! Michelle ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] (no subject)
Here are some links for HT/HTL licensure in FL. http://ww2.doh.state.fl.us/mqaservices/PractitionerServices.asp http://www.doh.state.fl.us/mqa/ClinLab/clp_applications.html There is a lot of information on that page, not just the application, so you might want to spend a few extra minutes looking around. Further down on the page, you can find the link related to the application. I've made it easier for you, by including the direct link here: http://www.doh.state.fl.us/mqa/ClinLab/ap_licensure.pdf The following URL is one resource for online CEUs. You can take the classes online, print your certificates and mail them in with your application, all on the same day! http://www.4ceuinc.com/home.asp?Profession=1 You will need to create a user id (using your email address is an easy way to remember your user name!) and password in order to see the next link. http://www.4ceuinc.com/courseDetails.asp?CourseId=344 This link takes you to the page for NEW FL licensee people. I would suggest you take the online courses, so you can print your certificate at the end On Sep 20, 2012, at 12:19 PM, Dorothy Glass techman...@yahoo.com wrote: What are the steps to applying and receiving a health care license to work in florida for an HT or an HTL? I heard there is also a requirement or different fees to be paid for a position as supervisor. Curious in Georgia. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Open Position - Laboratory Supervisor - Pathology
Please see the following link for more information and online application. https://uhs.ats.hrsmart.com/cgi-bin/portal/highlightjob.cgi?jobid=75412 Manatee Memorial Hospital, a 319-bed acute care facility is located on the banks of the Manatee River in Bradenton, Florida. The hospital has been a major provider of quality healthcare in the Tampa Bay Region since 1953. Manatee Memorial Hospital has a medical staff of over 500 physicians and the only residency program in the county. The spectacular four-story patient tower welcomes visitors as they cross over the bridge from Palmetto to Bradenton. The hospital offers a comprehensive range of medical services, including 24-hour emergency services, cardiac, cardiovascular, surgery, outpatient services, women’s and children’s services and orthopedic services, in addition to specialty programs in chest pain, a primary stroke center and the only pediatric center and Level ll Neonatal Intensive Care Unit in the county. Manatee Memorial Hospital currently has a Supervisor- Laboratory Pathology position available. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: disposal of formalin
We use Aldex, neutralize the formalin and dispose of the neutralized gel in the biohazard trash. The manufacturer says it can go in regular trash, but we choose to put it in biohazard trash. Michelle On Aug 17, 2012, at 4:18 PM, Vanessa Perez vpe...@pathreflab.com wrote: We use formalex green. http://americanbiosafety.com/PDF/ABS_FXG_Instructions.pdf is a link to the instructions for it we also keep a disposal log every time we dump the neutralized formalin down the drain.. Vanessa Perez Garcia Histology Supervisor Pathology Reference Lab 210-892-3746 210-892-3732 vpe...@pathreflab.com -Original Message- From: Johnson, Pamela [mailto:pamela.john...@stjude.org] Sent: Friday, August 17, 2012 3:09 PM To: Vanessa Perez; Lake,Debbie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: disposal of formalin May I ask what your neutralization protocol is? Pam Johnson, BS, HT (ASCP) Lab Manager Veterinary Pathology Core Lab St. Jude Children's Research Hospital 262 Danny Thomas Place Memphis, TN 38105-3678 Office - 901-595-3355 -Original Message- From: Vanessa Perez [mailto:vpe...@pathreflab.com] Sent: Friday, August 17, 2012 10:25 AM To: Lake,Debbie; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: disposal of formalin We neutralize it and then dump down the drain with tons of running water. Vanessa Perez Garcia Histology Supervisor Pathology Reference Lab 210-892-3746 210-892-3732 vpe...@pathreflab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lake,Debbie Sent: Friday, August 17, 2012 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] disposal of formalin Would anyone be willing to share how formalin is disposed of at your facility? Neutralization, disposal off site, etc. Thank you. Debra Lake MT(ASCP) Manager Micro, Blood Bank, Pathology Marion General Hospital Marion, IN 46952 (765) 660-6521 Fax: (765-651-7330) If you are not the intended recipient(s), you are notified that any disclosure, copying, distribution or any action taken or omitted to be taken in reliance on the contents of this information is prohibited and may be unlawful. If you receive this message in error, or are not the named recipient(s), please notify the sender, delete this e-mail from your computer, and destroy any copies in any form immediately. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Email Disclaimer: www.stjude.org/emaildisclaimer Consultation Disclaimer: www.stjude.org/consultationdisclaimer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Leica CV5030 coverslipper issues
We do have the Ventana labeled slides, but coverslip them by hand. The issues we have are with standard, non-labeled slides In fairness to both Leica and the repair company we use, it has been a couple of months since we have had a real problem. Not sure if that is saying a lot, just wanted to be fair. Michelle On May 22, 2012, at 5:40 AM, Angela Bitting akbitt...@geisinger.edu wrote: If you are using slides with labels (esp. Ventana's), don't think the upgrade will fix it. We tear our hair out most days of the week with our 2 CV5030s. I wish Ventana would just make a coverslipper to use with their labels or change their labels to something thinner. Symphony takes too long to use as a coverslipper. histot...@imagesbyhopper.com histot...@imagesbyhopper.com 5/21/2012 3:30 PM We have the CV5030 and have had *many* issues. I have a graveyard of the composite racks with broken ears on them. The machine has broken racks and slides, thrown coverslips, dropped slides and we kept being told that we just needed adjustments. Later we were told that a new electronics board upgrade would fix the issue. My understanding is that the upgraded board is approximately $4000! I love Leica products, but this particular unit has not lived up to their reputation. These issues began almost immediately after purchase and continue 5 years later. We keep getting it repaired, but I have told the repair company, it was my position that Leica should have stepped up and replaced the board free of charge given all the issues we have endured! Michelle On May 21, 2012, at 3:00 PM, Horn, Hazel V hor...@archildrens.org wrote: We have a CV5030 coverslipper and we have had a few issues but Leica has been quick to resolve them. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hor...@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Monday, May 21, 2012 1:15 PM To: 'Linda'; histonet@lists.utsouthwestern.edu Cc: kristy.han...@leica-microsystems.com; jack.ke...@leica-microsystems.com; paul.raimo...@leica-microsystems.com Subject: RE: [Histonet] Leica CV5030 coverslipper issues We've had some issues that were mostly due to operator errors and lack of housekeeping, but they have been very good about correcting the problems. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Linda Sent: Monday, May 21, 2012 1:58 PM To: histonet@lists.utsouthwestern.edu Cc: kristy.han...@leica-microsystems.com; jack.ke...@leica-microsystems.com; paul.raimo...@leica-microsystems.com Subject: [Histonet] Leica CV5030 coverslipper issues Hello Everyone, I purchase a brand new Leica CV5030 coverslipper, which I received at the beginning of February. I have had non- stop issues with this coverslipper from it throwing slides, coverslips and now the sensor not working properly. I have emailed Leica several times now requesting a new coverslipper. If I have this many issues at three months what is it going to do in a year? The tech service support person has been great with fixing all of the issues. This is my reply I received from the President, North America, Jack Kenny- We do not believe that it is appropriate at this point to replace this system. We will continute to monitor the situation but not upgrade at this time. I am not looking for an upgrade I would just like a new coverslipper that works. Has anyone else had problems with Leica not replacing defective equipment? Please let me know. How did you resolve the issue? Thank you in advance, Linda Dee, BGS, HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cytology Staining
Is Cyto prep considered a high complexity task? On May 17, 2012, at 12:59 PM, Yang, Mari my...@emc.org wrote: Sheila, Maybe the inspector was referring to the prep? Many cytology labs use automated staining. If you find out any information, I'd love to know. Thanks, Mari Mari Yang, MHA, CT(ASCP)CMHTLCM Cytology Supervisor Tel: 760.773.2009 P Save a tree, please don't print this e-mail unless you really need to. Confidentiality Note: The preceding e-mail message (including any attachments) contains information that may be confidential, protected by applicable legal privileges, or constitute non-public information. It is intended to be conveyed only to the designated recipient(s). If you are not an intended recipient of this message, please notify the sender by replying to this message and then delete it from your system. Use, dissemination, distribution or reproduction of this message by unintended recipients is not authorized and may be unlawful. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Thursday, May 17, 2012 4:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cytology Staining Hello all. During an inspection yesterday, the inspector made a brief, vague reference to cytology staining now being deemed high complexity. Does anyone have information and/or a reference for this? I'd appreciate any information anyone could provide. Thanks a bunch. Sheila Haas Laboratory Manager MicroPath Laboratories, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Leica CV5030 coverslipper issues
We have the CV5030 and have had *many* issues. I have a graveyard of the composite racks with broken ears on them. The machine has broken racks and slides, thrown coverslips, dropped slides and we kept being told that we just needed adjustments. Later we were told that a new electronics board upgrade would fix the issue. My understanding is that the upgraded board is approximately $4000! I love Leica products, but this particular unit has not lived up to their reputation. These issues began almost immediately after purchase and continue 5 years later. We keep getting it repaired, but I have told the repair company, it was my position that Leica should have stepped up and replaced the board free of charge given all the issues we have endured! Michelle On May 21, 2012, at 3:00 PM, Horn, Hazel V hor...@archildrens.org wrote: We have a CV5030 coverslipper and we have had a few issues but Leica has been quick to resolve them. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hor...@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Monday, May 21, 2012 1:15 PM To: 'Linda'; histonet@lists.utsouthwestern.edu Cc: kristy.han...@leica-microsystems.com; jack.ke...@leica-microsystems.com; paul.raimo...@leica-microsystems.com Subject: RE: [Histonet] Leica CV5030 coverslipper issues We've had some issues that were mostly due to operator errors and lack of housekeeping, but they have been very good about correcting the problems. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Linda Sent: Monday, May 21, 2012 1:58 PM To: histonet@lists.utsouthwestern.edu Cc: kristy.han...@leica-microsystems.com; jack.ke...@leica-microsystems.com; paul.raimo...@leica-microsystems.com Subject: [Histonet] Leica CV5030 coverslipper issues Hello Everyone, I purchase a brand new Leica CV5030 coverslipper, which I received at the beginning of February. I have had non- stop issues with this coverslipper from it throwing slides, coverslips and now the sensor not working properly. I have emailed Leica several times now requesting a new coverslipper. If I have this many issues at three months what is it going to do in a year? The tech service support person has been great with fixing all of the issues. This is my reply I received from the President, North America, Jack Kenny- We do not believe that it is appropriate at this point to replace this system. We will continute to monitor the situation but not upgrade at this time. I am not looking for an upgrade I would just like a new coverslipper that works. Has anyone else had problems with Leica not replacing defective equipment? Please let me know. How did you resolve the issue? Thank you in advance, Linda Dee, BGS, HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] cassette marker
I like the StatLab markers. On Dec 3, 2011, at 1:38 PM, Rathborne, Toni trathbo...@somerset-healthcare.com wrote: Can anyone recommend a marker for using on cassettes? We currently use pencil, which sometimes smudges. We've tried a few markers already, but some fade, while others hold up well for processing, but won't when placed in decalcifier. Vendors are welcome to respond. Thanks, Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Difference between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4%
Wow. Just Wow! :o( Sent from my iPhone On Oct 5, 2011, at 7:03 PM, Jenny Vega histotech...@gmail.com wrote: Ok I want to know the diferrence between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4% . I was taugh at college that they were the same thing and in the book from Frieda Carson it says that but my supervisor swears they are different chemicals. In the laboratory she has used 10% neutral buffered formalin all the time, but when we ran out of it we were sent NB formaldehyde 3.7 to 4 %she sent it back and we were sent NB formaldehyde 3.7 to 4% once again. She says that 10% NBF is more concentrated and pure than 3.7 to 4 % formaldehyde and that the tissues are going to putrefied if they are put in that solution. Am I wrong or is she wrong? thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Automatic HE stainer with coverslipper (glass)
We use the Leica autostainer, bridge coverslipper. We use the premimum coverslips from Fisher and seldom have any issues with them sticking together (and we're in FL!) Does anyone have issues with the coverslipper breaking off the ears on the grey/black racks? I have a cupboard graveyard for my broken racks. :o( Michelle Sent from my iPhone On Sep 2, 2011, at 5:48 AM, Bartlett, Jeanine (CDC/OID/NCEZID) j...@cdc.gov wrote: We use high quality slides from Mercedes Medical with no problems at all. We also use traditional slidesno rounded corners and have no problems. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Fleming, Jackie M Sent: Thursday, September 01, 2011 4:41 PM To: Bea DeBrosse-Serra; 'Ernestine Middleton'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Automatic HE stainer with coverslipper (glass) Must use the Leica coverslips - OR it's a lot of downtime! Stainer is slower than we had hoped, but we have a really high volume. Great service! Jackie Fleming HT ASCP Allina Medical Laboratries Histology Technical Consultant phone: 612-863-4773 pager: 612-654-2135 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Bea DeBrosse-Serra [bdebrosse-se...@isisph.com] Sent: Thursday, September 01, 2011 3:38 PM To: 'Ernestine Middleton'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Automatic HE stainer with coverslipper (glass) Leica...all the way! Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 1896 Rutherford Road Carlsbad, CA 92008 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ernestine Middleton Sent: Wednesday, August 31, 2011 6:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automatic HE stainer with coverslipper (glass) Hi; Looking and need comments on those of you that are using combination HE stainer with glass coverslipper. Thank you. Ernestine Middleton, Manager, HT, HTL ,BS ,MPA Montefiore Medical Center Bronx, New York 718-920-4157 emidd...@montefiore.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message contains information that is confidential and may be privileged. Unless you are the addressee (or authorized to receive for the addressee), you may not use, copy or disclose to anyone the message or any information contained in the message. If you have received the message in error, please advise the sender by reply e-mail and delete the message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] training techs
Way to go Patsy!! :o)
Sent from my iPhone
On Aug 29, 2011, at 5:36 PM, Patsy Ruegg pru...@ihctech.net wrote:
Hey Everyone,
The third tech I trained in the last 3 years just passed her HTL exam last
Friday, that all makes me feel pretty good. I just signed an affiliate
agreement to be a clinical training site for the Histology program at UND
today, they are sending me another student who will be with me for 2
semesters.
Regards,
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email mailto:pru...@ihctech.net pru...@ihctech.net
web site http://www.ihctech.net www.ihctech.net
This email is confidential and intended solely for the use of the Person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author. It may contain information that is
privileged confidential within the meaning of applicable law. Accordingly
any dissemination, distribution, copying, or other use of this message, or
any of its contents, by any person other than the intended recipient may
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Re: [Histonet] peggy wenk comments on HT/HTL practical
Interestingly, I have never been required to section tissue in a job interview. I have worked at four hospitals, three private labs and one research facility (hubby moved us around a bit!) I just recently hired two techs, directly out of school with no real world experience. I did ask them to cut some slides for me! ;o) Michelle Sent from my iPhone On Aug 29, 2011, at 9:05 AM, joelle weaver joellewea...@hotmail.com wrote: I was required to know and understand all this as well. I often have had to perform various aspects on an interview, and had no problems with this. Joelle Joelle Weaver MAOM, BA, (HTL) ASCP From: b-freder...@northwestern.edu To: lpw...@sbcglobal.net; mad...@verizon.net; histonet@lists.utsouthwestern.edu Date: Mon, 29 Aug 2011 12:35:50 + Subject: RE: [Histonet] peggy wenk comments on HT/HTL practical CC: I remember having to know, regarding Peggy's comment on why a reagent was on a piece of tissue, for my HTL what was going in every step of the retic (oxidation,reduction,toning etc) and believe me it was on the exam. We were taught to know the why from the techs that trained us and had taken the exam. If you chose to ignore what they said, it was on your head. As to the microtomy during an interview, I'm all for it as I have done it in the past (as an interviewer and interviewee) and most recently, as we had a tech come in from Romania and how were we to know what she knew? Their program is a CLS degree and she chose histo from that. Great tech by the way- histo is not much different the world over, from what I can see. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee Peggy Wenk Sent: Sunday, August 28, 2011 6:41 PM To: mad...@verizon.net; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] peggy wenk comments on HT/HTL practical Now add in a few other things, that I didn't directly mention in the original 2006 HistoNet reply. How many people are now using: - automated HE stainers - automated special stainers, including IHC stainer - automated coverslippers - automated microtomes - disposable blades Can you see why nearly everyone passed the practical? If they could cut a half-way decent section (with better microtomes and blades, easier to do), just put the slide on a machine and let the machine stain it. To fail, they basically had to NOT follow a LOT of directions, such as submitting colon for small intestine, submitting autolyzed gall bladder, doing the wrong stain (like doing a Prussian blue for iron, instead of the requested colloidal iron), grossing the tissue too small, microtoming too thick, putting the institution's name on the label, etc. Automation makes it easier to produce better sections and better stains, particularly if someone is a mediocre tech to begin with. As to whether the person understands the theory when using automated stainers - well, the fact that many of the people submitting the practical could pass the practical but would fail the written - that has been going on since ancient times, when staining was done by hand. They could follow the directions, but didn't know the reasons. Pour on solution A for 5 minutes, pour it off, pour on solution B for 10 minutes, rinse it off, and it's done. No idea what is in solution A or B, or what chemicals are binding to what components in the tissue, etc. Whether someone is doing the stain by hand or by machine, it's up to the person to have the curiosity to find out what is going on. Some people don't have it, and don't feel the urge to learn. That's one of the reasons I like going to state and national meetings, and reading HistoNet. These people WANT to learn! Hurrah for them! And there are a lot of people in the histology community willing to help people who WANT to learn - answering HistoNet, giving talks at state and national meetings, being a mentor, etc. Hurrah for them too! Peggy A. Wenk, HTL(ASCP)SLS Beaumont Health Systems Royal Oak, MI 48073 The above are my opinions and not those of my institution. -Original Message- From: mad...@verizon.net Sent: Sunday, August 28, 2011 2:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] peggy wenk comments on HT/HTL practical The dropping of the practical was explained so well by Peggy Wenk. I never got into the debate(glad I did not after reading Peggys comments), I would have argued to keep it. That said, indeed it is outdated. The fact that we were the last to do it I guess speaks volumes. I did struggle getting
Re: [Histonet] harrasment for humble histotechs
:o). I'm not even sure I could find my results after all these years and 9 moves! I *do* remember how picture perfect those slides had to be though. Tiny air bubble=graded down. Folds=forget it. Knife marks=bad. I did both the HT and the HTL practicals and now new students don't even have to submit them. I didn't cheat, but over the years I saw students attempting to cheat. If I saw them, I would remind them that they were to work independently, but who knows what they did when I wasn't looking? Integrity is what you do when no one is looking. Sent from my iPhone On Aug 27, 2011, at 3:25 PM, mad...@verizon.net wrote: Gentle harrassment. I encourage you to look at you scores for your practical and written exam. Between gigs I am getting my important documents together and found my old scores for the HTL, no thte HT, however, both parts of the exam were taken by yours truly. Never got into the talk of practical vs no practical. I do think it si a good idea to keep it. Honest techs will be proud to do a practical. Looking at scores made me feel good that the next great job is right around the corner. Nick(Rocky) Madary, HT/HTL(ASCP)QIHC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Returning to Histology-Another Idea Won't Work
Sorry, I misread, the experience must be within the last 10 years. Michelle Sent from my iPhone On Aug 27, 2011, at 1:45 PM, Lee Peggy Wenk lpw...@sbcglobal.net wrote: It looks like the time limits prohibit me from trying this, since I did it so long ago What is IT? What is prohibiting you from taking the HTL exam? What does having taken and passed the HT exam have to do with taking the HTL exam? Sorry, I'm really lost as to what you need, and how to help you. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 The above response reflects my opinion, and not the hospital's view. -Original Message- From: Paula Sent: Thursday, August 25, 2011 3:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Returning to Histology-Another Idea Won't Work I thought it might be good to study and try for the HTL (already have school for HT and am certified) but it looks like the time limits prohibit me from trying this, since I did it so long ago. Anyone else have any ideas? I'd gladly buy the books and study. Cannot get into a lab here in NC without current experience. The closest school is in western NC, about 5 hours away. I didn't see anything near me in Raleigh. I was going to purchase the textbooks but now I see this won't work either: Histotechnologist, HTL(ASCP) Application Fee: $210 To be eligible for this examination category, an applicant must satisfy the requirements of at least one of the following routes: Route 1: Baccalaureate degree from a regionally accredited college/university with a combination of 30 semester hours (45 quarter hours) of biology and chemistry AND successful completion of a NAACLS accredited Histotechnician or Histotechnology program within the last 5 years; OR Route 2: Baccalaureate degree from a regionally accredited college/university with a combination of 30 semester hours (45 quarter hours) of biology and chemistry AND one year full time acceptable experience in a histopathology (clinical, veterinary, industry or research) laboratory in the U.S., Canada or an accredited laboratory* within the last ten years. *laboratory accredited by a CMS approved accreditation organization (i.e., AABB, CAP, COLA, DNV, The Joint Commission, etc.) Clinical Laboratory Experience To fulfill the experience requirement for the Histotechnologist examination, you must have experience, within the last ten years, in the following areas: Fixation Microtomy Processing Staining Any ideas? I have posted this before, but I keep on trying! Paula ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Returning to Histology-Another Idea Won't Work
10 years experience to qualify for taking the exam? Is that an ASCP requirement? It sounds like they might be exchanging experience for the bachelor's degree? Have you checked out the online courses? I believe there are some that offer the bachelor's degree. Michelle Sent from my iPhone On Aug 27, 2011, at 1:45 PM, Lee Peggy Wenk lpw...@sbcglobal.net wrote: It looks like the time limits prohibit me from trying this, since I did it so long ago What is IT? What is prohibiting you from taking the HTL exam? What does having taken and passed the HT exam have to do with taking the HTL exam? Sorry, I'm really lost as to what you need, and how to help you. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 The above response reflects my opinion, and not the hospital's view. -Original Message- From: Paula Sent: Thursday, August 25, 2011 3:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Returning to Histology-Another Idea Won't Work I thought it might be good to study and try for the HTL (already have school for HT and am certified) but it looks like the time limits prohibit me from trying this, since I did it so long ago. Anyone else have any ideas? I'd gladly buy the books and study. Cannot get into a lab here in NC without current experience. The closest school is in western NC, about 5 hours away. I didn't see anything near me in Raleigh. I was going to purchase the textbooks but now I see this won't work either: Histotechnologist, HTL(ASCP) Application Fee: $210 To be eligible for this examination category, an applicant must satisfy the requirements of at least one of the following routes: Route 1: Baccalaureate degree from a regionally accredited college/university with a combination of 30 semester hours (45 quarter hours) of biology and chemistry AND successful completion of a NAACLS accredited Histotechnician or Histotechnology program within the last 5 years; OR Route 2: Baccalaureate degree from a regionally accredited college/university with a combination of 30 semester hours (45 quarter hours) of biology and chemistry AND one year full time acceptable experience in a histopathology (clinical, veterinary, industry or research) laboratory in the U.S., Canada or an accredited laboratory* within the last ten years. *laboratory accredited by a CMS approved accreditation organization (i.e., AABB, CAP, COLA, DNV, The Joint Commission, etc.) Clinical Laboratory Experience To fulfill the experience requirement for the Histotechnologist examination, you must have experience, within the last ten years, in the following areas: Fixation Microtomy Processing Staining Any ideas? I have posted this before, but I keep on trying! Paula ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cleanser for blades/instruments
Liquinox and bleach soulution works for us. Michelle Sent from my iPhone On Aug 26, 2011, at 6:24 PM, Jeter, Brent brent.je...@gwu-hospital.com wrote: Histonetters, Can anyone recommend a good cleanser for soaking scissors/blades/instruments? Thanks in advance - Brent Jeter Anatomic Pathology Supervisor The George Washington University Hospital 202-715-5076 (phone) 202-715-4691 (fax) brent.je...@gwu-hospital.comhttps://mail.uhsinc.com/owa/redir.aspx?C=2e2500d5f8774abea919776f9edf4c8dURL=mailto%3abrent.jeter%40gwu-hospital.com UHS Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient (s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution of this information is prohibited. If this was sent to you in error, please notify the sender by reply e-mail and destroy all copies of the original message.___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cassette Marking Pen
Statlab pens for both cassettes (when we hand write them) and for slides. Michelle Sent from my iPhone On Aug 22, 2011, at 11:26 AM, Sherwood, Margaret msherw...@partners.org wrote: We use the marking pencils, exlusively, for our cassettes and StatLab marker pens for our slides. Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of dkb...@chs.net Sent: Monday, August 22, 2011 11:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassette Marking Pen I know I have seen this thread before, but what marking pens are you using to write on your cassettes? We are using the KP Marker Plus from Mercedes and haven't had a problem until this shipment. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkb...@chs.net -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Shipping prepared histology slides
Good gosh, don't tell that to the reference labs who routinely send out finished slides!! Sent from my iPhone On Aug 10, 2011, at 5:30 PM, b427...@aol.com wrote: Can someone provide a quick reference to ANYTHING I can use to prove to my EHS that fixed, processed, coverslipped slides are not HAZMAT? They insist shipping finished slides cannot be performed by histotechs. Wha? Thanks. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Re: Knife for trimming paraffin from blocks
I agree, the Para-Trimmer is the next best thing since sliced bread! I'll never go back to the knife again. We use lid from one of the Ventana special stains boxes to catch the wax. I have also fashioned a drip tray out of the cardboard boxes that the coverslips come in. We're all McGuyver's at heart, aren't we? :o) Sent from my iPhone On Aug 8, 2011, at 11:14 AM, John Shelley jshel...@sanfordburnham.org wrote: Hi All, I likewise use the Para-trimmer and instead of a specimen cup I just use the top lid of a 1/2 gross microscope slide box. Once filled I just throw away in the trash. Kind Regards! John J Shelley Senior Research Associate, Histology Core -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cormier, Kathleen Sent: Monday, August 08, 2011 11:04 AM To: gayle callis; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Knife for trimming paraffin from blocks We use the paratrimmer too. What we do for a paraffin catcher is to use a specimen cup (I call them urine cups, but whatever) to catch the paraffin. When it get fullish, we place in the slide oven to melt the paraffin, when melted pour off, and reuse the cup... :) Kathy Cormier Histology Manager Charles River Laboratories 251 Ballardvale Street Wilmington, MA 01887 Ph: 781-222-6803 Fax: 978-988-8793 kathleen.corm...@crl.com Accelerating Drug Development. Exactly. Notice - This email and any files transmitted with it are confidential and may contain privileged and/or proprietary information. You must not disclose this message to another party without Charles River's express written consent. If you are not the intended recipient you must not copy, distribute or use this email or the information contained in it for any purpose other than to notify us. If you have received this message in error, please notify Charles River immediately, and delete it from your system. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of gayle callis Sent: Monday, August 08, 2011 10:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Knife for trimming paraffin from blocks You Wrote: I am looking for a stainless steel knife that we use to scrape off the paraffin from the embedded blocks. I could not find the 6 inches size knife in any catalog. Thanks in advance Mala Nirmala Srishan Histology Supervisor Holy Name Medical Center. * Dear Mala, In the past, we used an old style permanent edge scalpel blade but after a disaster with one person seirously cutting himself while trimming paraffin from a block, we purchased a Paratrimmer with a heated, slanted metal surface. This trimmer has made everyone happy by eliminating the potential for serious injury and does an even better job of getting rid of excess paraffin. There are two models sold, one from Thermo Scientific and the other one was recently spotted on a vendor website. (Sorry, I didn't jot the name down). The trimmers are worth the investment to keep you and your employees safe from nasty cuts, no matter what the knife/blade, etc could be used. The only drawback to the Para Trimmer is the messy paraffin drippings have to be collected in some separate container that the manufacturer doesn't supply. This is more than a bit annoying. It seems to me the manufacturer of these devices would design the trimmer with a paraffin catch tray. A small aluminum baking pan (from grocery store) could be used and disposed of (not a Green consideration). Be safe rather than sorry. Gayle M. Callis HTL/HT/MT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] (no subject)
Ruthie, In order to unsubscribe from the list, you must click on the link at the bottom of the email, scroll to the bottom of the webpage and enter your email requesting to be unsubscribed. You have the power to unsubscribe yourself! Good Luck! Michelle Sent from my iPhone On Jul 26, 2011, at 12:50 AM, Ruthie Wilson wilsonj...@yahoo.com wrote: Please unsubscribe me ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HE protocol question
Hi Histonetters! I'm looking for thoughts on preferences/pros/cons between using a progressive and a regressive HE on routine daily work. Which hematoxylins do you prefer (commercially prepared), which eosin? Anyone have a tried and true protocol for each method? Thanks! Michelle Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Recycled alcohol
Hi Histonetters! For those who use recycled alcohol, I have a few questions. Do you use it in your automatic HE stainers? Are you having any troubles with proper, consistent HE staining? We are using recycled alcohol, and wish to continue to do so IF we can. We are having issues with our staining - hematoxylin isn't as purple as it should be, eosin is way too pink/red. We use recycled alcohol when deparaffinizing and running down to water. For the record, we are also using recycled xylene. After staining, we use 2 recycled 100s and two fresh 100s. I have increased the hem time, decreased the eosin time, adjusted the acid alcohol and bluing... I'm tearing my hair out trying to troubleshoot this! Can anyone help me? Thanks! Michelle ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] NBF waste disposal
Sharon, We use a product called Aldex to neutralize our formalin. It binds with the aldehyde groups and neutralizes them. According to the mfr, you can dispose of it in regular trash, but I am more cautious and throw it away in red bag trash. Michelle Sent from my iPhone On Jul 22, 2011, at 1:01 PM, Campbell, Sharon scampb...@celligent.net wrote: Happy Friday everyone, I am currently researching ways to dispose of NBF waste. I would like to know if you are neutralizing the waste and then putting it down the drain or if you are shipping it off to be handled by another company. I am looking for the best and most cost effective way to handle this waste. We are not in a position to reclaim the formalin as space is not available for the still. Thank you for your help on this. Sharon Campbell Sharon Campbell HT, HTL (ASCP) Histology Supervisor Celligent Diagnostics, LLC 106 Venture Blvd. Spartanburg, SC 29306 (864) 583-3850 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Assisting with Autopsies
We do, on average, about 15 autopsies per year. We have a diener who does them for us. The pathologists are present and are often are hands on during the autopsy. Sometimes a lab aide will be there in the mode of a scribe. Our histotechs do not participate in autopsies. Michelle On Jul 20, 2011, at 6:06 AM, Podawiltz, Thomas tpodawi...@lrgh.org wrote: We have always helped with autopsies, just comes with the territory. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of susan.wal...@hcahealthcare.com Sent: Wednesday, July 20, 2011 3:32 AM To: as...@georgetownhospitalsystem.org; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Assisting with Autopsies We are a small hospital and though we no longer do in house autopsies we still had an autopsy assistant.(most prefer to be called this) We always had a pool of people available to do this job. Training as a histotech does not include this job and I have always refused to do it. I know there are techs who do not mind and some who supplement their income doing it but histotechs should not be forced to do them, certainly not free of charge. If enough histotechs in an area stand together and refuse the pathologists will find assistants. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Tuesday, July 19, 2011 3:12 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Assisting with Autopsies Hello All, We are small hospital that does approximately 5-10 autopsies a year. This being said our administration department does not want to hire a diener to assist with these autopsies. So I have decided to turn to all of you out there in histoland for a little poll. Does your facility use histotechs or a diener to assist with the autopsy? Thanks in advance for all of your help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: AW: [Histonet] Quick Drying Mounting Media
Have you tried drying them under a blow dryer? That's how we used to dry our slides before we sent them out. It was a commercially available slide dryer. Michelle Sent from my iPhone On Jul 14, 2011, at 3:57 AM, Gudrun Lang gu.l...@gmx.at wrote: I think it's more depending on the organic solvent used before coverslipping. We use butyl acetate in the last trogh in the stainer and in the coverslipper. This reagent dries/ evaporates very fast. So after 15 min there is hardly a smell of it and we deliver our slides immediately. We use Pertex as coverslipping medium. Gudrun Histolab, Akh Linz, Austria -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Katelin Lester Gesendet: Mittwoch, 13. Juli 2011 19:42 An: Histonet Betreff: [Histonet] Quick Drying Mounting Media Hi histonet, We have a brand new Leica CV5030 Coverslipper and I am looking for the quickest drying media available. We are currently using Micromount by Surgipath/Leica and it is not drying fast enough. Any suggestions are appreciated as we have to package our slides for shipping and the media is either sticking to the bubble wrap or creating circles on the slides from the pressure of the bubble wrap. -- Katelin Lester, HTL Gastroenterology Specialists of Oregon, P.C. Pathology Laboratory (971) 224-2408 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Waste disposal
For those who use the Ventana IHC and special stainers, how do you dispose of the liquid waste that is generated? Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Re: Embedding Media
Amita, The paraffin that we use is also a Leica product. It was formerly sold under the Surgipath label, but since to companies have merged together, it now sells under the Leica name. It is a beaded pellet paraffin and has a version suitable for infiltration and a version suitable for embedding. The box of the embedding paraffin suggests it can be utilized for *both* purposes, however we do not choose to use it that way. So yes, we use two different types of paraffin. Hope that helps! Michelle On Jul 11, 2011, at 2:07 AM, amitapan...@torrentpharma.com wrote: I continuation of same discussion , i would like to have clarification for our lab..we use beaded paraffin Leica Paraplast both for processing and embedding . Do you advice us to use different paraffin for both steps? Paula, Please share if you get any feed back on Richard Allan product. Amita From: Tim Higgins thigg...@cddmedical.com To: histonet@lists.utsouthwestern.edu Date: 07/08/2011 11:34 PM Subject:[Histonet] Re: Embedding Media Sent by:histonet-boun...@lists.utsouthwestern.edu Hi Paula, Obviously there are no compression free paraffin's on the market. Look for a paraffin with a higher polymer content, this makes the paraffin firmer and less likely to compress. Other factor for into section compression but paraffin is a good place to start. The higher polymer paraffin's are not as suited for processing, it is more for the embedding portion of the process. Use a paraffin with lower polymers content in the processor and higher for embedding and you will like the results. Tim Date: Fri, 8 Jul 2011 09:44:51 -0700 From: Paula Lucas plu...@biopath.org Subject: [Histonet] Embedding Media To: histonet@lists.utsouthwestern.edu Message-ID: 9251EDA88CA748D6861A0C3BB0E32FCB@biopath.local Content-Type: text/plain; charset=us-ascii Hello, We are considering a switch to a different brand of paraffin and this is because I feel we are having too many compressions in some of our tissue sections. Currently, we use Tissue Path Paraplast, regular. I would like to get feedback from you as to what you prefer. Looking on line, Richard Allan has a product called Signature Series Paraffin that comes in a type L that offers compression-free sections and I was also hoping to get any feedback on that product. I would greatly appreciate any suggestions and thoughts. Thanks in advance, Paula ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Test
CONGRATS!!! :-D Sent from my iPhone On Jul 11, 2011, at 12:08 PM, sdys...@mirnarx.com wrote: So I got married and my name changed (meaning so did my email) tried sending out a broad email, but it didn't work. Just making sure I'm updated now =) Happy Monday all!! Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Embedding Media
We use the infiltration embedding paraffin from Surgipath/Leica and are very happy with it. Michelle Sent from my iPhone On Jul 8, 2011, at 12:44 PM, Paula Lucas plu...@biopath.org wrote: Hello, We are considering a switch to a different brand of paraffin and this is because I feel we are having too many compressions in some of our tissue sections. Currently, we use Tissue Path Paraplast, regular. I would like to get feedback from you as to what you prefer. Looking on line, Richard Allan has a product called Signature Series Paraffin that comes in a type L that offers compression-free sections and I was also hoping to get any feedback on that product. I would greatly appreciate any suggestions and thoughts. Thanks in advance, Paula ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Isopropyl alcohol
Rene, I was just as surprised! When we purchased the mw, we were led to believe that we could use the recycled alcohol, but I was told later that we should not for quality reasons - that recycled is never 100% pure and that water contamination will adversely affect the processing. So, can I use it on my HE stainer without negatively affecting the stain quality? As always, thanks for you thoughts, Michelle On Jul 5, 2011, at 11:58 AM, Rene J Buesa rjbu...@yahoo.com wrote: First of all, if you recycle correctly your 2-propanol there is no objective reason why you cannot use it in your MW processor, unless your provider wants you to buy your 2-propanol from them. René J. --- On Tue, 7/5/11, histot...@imagesbyhopper.com histot...@imagesbyhopper.com wrote: From: histot...@imagesbyhopper.com histot...@imagesbyhopper.com Subject: [Histonet] Isopropyl alcohol To: Histonet@Lists. Utsouthwestern. Edu histonet@lists.utsouthwestern.edu Date: Tuesday, July 5, 2011, 10:42 AM We recycle our isopropyl alcohol. We have been told by our microwave manufacturer that we cannot use the recycled alcohol on the microwave processor. As a result, I am trying to find additional uses for the recycled isopropyl. Is there any reason why I can't use the iso in place of ethanol when running the slides down to water on the HE stainer? Any others uses? Any input is greatly appreciated! :o) Michelle ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Isopropyl alcohol
We recycle our isopropyl alcohol. We have been told by our microwave manufacturer that we cannot use the recycled alcohol on the microwave processor. As a result, I am trying to find additional uses for the recycled isopropyl. Is there any reason why I can't use the iso in place of ethanol when running the slides down to water on the HE stainer? Any others uses? Any input is greatly appreciated! :o) Michelle ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Could you please recommend? (Bascaramurty, Saro)
I second the Leica! :o) Michelle Sent from my iPhone On Jun 29, 2011, at 1:14 PM, White, Lisa M. lisa.whi...@va.gov wrote: We use the Leica CM1850 UV. It is easy to use for sectioning as well as UV decontamination. Pricing fell in line with other vendors. I have used Shandon, Leitz, Leica and a unit so old I think it came with Noah on the boat don't even remember the maker. Leica is the favorite. It sections well and was the first one that the anti-roll plate would work correctly. It decontaminates well. No regrets, love it and would recommend. Lisa White, HT(ASCP) Supervisory HT James H. Quillen VAMC PO Box 4000 Corner of Veterans Way and Lamont PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] How many tissues an histo tech is suppose to cut per
OMG, 100 blocks/hour? Seriously? I am fast , but even I can't touch that! That's simply insane!! :o( Sent from my iPhone On Jun 28, 2011, at 6:20 PM, Grantham, Andrea L - (algranth) algra...@email.arizona.edu wrote: I'd do it - along with Rene's paper which was published and the Canadian paper that was referenced yesterday. Ask these people - if they would want their child's tissue bx treated in this manner? They have no clue as to what we do - you might suggest that you put together an inservice for the lab managers on histopathology. Not hard to do using a little power point, you can take your own pictures of the steps that the tissue samples go through. Emphasize that cutting something like a fallopian tube is different than cutting cervix or bone. They might be happy that you are interested enough in providing insight into histopathology. I have something like this that I use often when speaking to service groups and high school students in my community. If they don't want to consider all that you have to do - look for another job! I had a problem with the OR people one time so I did a presentation for them on the importance of fixation and I took them through all the steps from patient to slide to pathologist. They were floored because they never thought it was such an involved process, and my tissues were treated much better by the OR staff. Andi On Jun 28, 2011, at 1:49 PM, Stella Mireles wrote: Joanne is not alone. The lab I work at has informed us that due to their own research, a single histotech should be able to do 100 blocks an hour. I'm not sure if they expect embedding, trimming, writing slides, sectioning and manual staining in this goal. I am a seasoned histotech, and have tried to speak to my lab manager and lab supervisor, (both are med. techs and have very little insight into what is involved in producing a high quality slide), but their goal remains the same. I have an idea: I feel like printing some of your responses to Joanne question and showing them that their goal is unattainable as well. Any other suggestions. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] How many tissues an histo tech is suppose to cut per
Not to add fuel and all that... but I can, and regularly do, cut single slide blocks in less than minute per block. I am not a new tech though, been around the microtome a few times! I think the proper approach would be to have the tech *average* the number of slides the cut over a period of time. Then watch the trend, are you showing improvement over time? I think forward progress is what should be watched for, while maintaining quality. Quality is prarmount. Sent from my iPhone On Jun 25, 2011, at 6:27 PM, Rittman, Barry R barry.r.ritt...@uth.tmc.edu wrote: Joanne I assume that you are not having us on as this seems a ridiculous goal to set. I would ask to look at previous personnel records to determine where the employer managed to find a robot that did this previously and ask your supervisor to show how he/she cuts one block per minute. It is obvious that the time taken to carry out cutting depends to a large extent on the challenge of the tissue blocks. It is also obvious that with such an attitude there will be less attention to quality and a greater likelihood of errors occurring.I would point out to management the potential lawsuits that might occur if a mistake is made and I suspect that they have not thought this through at all or are just trying to see how far they can go. An alternate solution would be for you to ask for a set sum per block and see their response. 53 is no age at all kid so hang in there and if you get an unfavorable response from management point out the big job market out there and the severe lack of skilled histotechs. My response to them would be more graphic. Barry From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joanne [joanne0...@comcast.net] Sent: Saturday, June 25, 2011 4:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How many tissues an histo tech is suppose to cut per i've only been working 2 months. although older, i am new as a histotech (graduated in may 2010, found a job in april 2011). seems management is setting a goal of a block per minute as far as cutting goes for me. i have until october to attain this goal. this minute for cutting is to include facing, writing out slides, cutting, and putting tray into symphony stainer (not to mention getting up to answer the phone, fielding questions regarding send-out cases, and other slight cutting interruptions). this seems an extreme, possibly unattainable goal. i'm up for a challenge at age 53, but any advice would be SWONDERFUL :) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Retirement
And remember mouth pipeting? oops, that's the cotton... Eating and smoking in the lab was the norm. Our alcohol had the tax stamp on it! :o) Michelle On Jun 19, 2011, at 4:19 PM, Paula Sicurello pat...@gmail.com wrote: Sheesh! We used to have people smoke while working with propylene oxide. Eating in your control pigs was part of the benefit of being a graduate student to save on grocery money. Film? My TEM used glass plates. Lab mates used to routinely drink diet coke and 100% ethanol on Fridays. Wearing closed toed shoes was for wimps, you were just fast if you dropped a steel wedge blade. We even wrote using the entire word and proper grammar, none of this acronym stuff for us. Retirement? What's that? Paula :-) On Sun, Jun 19, 2011 at 12:31 PM, Amos Brooks amosbro...@gmail.com wrote: Agarose Gels! ... Listen you whipersnapper Agarose is the easy way out. When I learned it we used to have to make up our own polyacrylamide gels. That was after having to walk to work up hill both ways in 30 feet of snow! (No nearer retirement) Crotchety Amos Message: 7 Date: Fri, 17 Jun 2011 13:24:12 -0400 From: Emily Sours talulahg...@gmail.com Subject: Re: [Histonet] Retirement To: histonet@lists.utsouthwestern.edu Message-ID: banlktinejxtxyop-byfweuxn3yw-ff3...@mail.gmail.com Content-Type: text/plain; charset=UTF-8 Retirement? I think by the time I get to that point, social security will have run out. Then again, technology will be so advanced, I can tell stories about the old days, where I logged on to the bbs by modem to post messages to my friends and typed in my own html coding. We didn't have google when I was young!! Our cameras used film! And you couldn't see how bad your pictures were until you developed that film!! There was no PCR to sequence your DNA, you ran an agarose gel and hoped for the best!! You could drink the 100% ethanol, there was no denaturing! (okay that was before my time) You could smoke in the lab while you sectioned without gloves!! (okay that was too) Emily A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] (no subject)
Dorothy, Is your question related to the fact that a protocol will have the currently active lot number and you want to use a different lot number? Or is this a brand new antibody that has not been in use before and therefore has not ever been validated? Michelle On Jun 15, 2011, at 11:41 AM, Dorothy Glass techman...@yahoo.com wrote: Dear Histonets, How is it possible to validate a antibody on the Ventana Ultra or XT, when the antibodys afre prediluted or made into a prep-kit? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Keeping Histo room floor clean?
We don't keep it off the floor, but do use a wide-bladed putty knife attached to a mop handle to scrape the residual wax off the floor. It woks quite nicely and doesn't remove the actual floor wax like a razor blade scrapper would. Michelle Sent from my iPhone On Jun 14, 2011, at 6:44 PM, JR R rosenfeld...@hotmail.com wrote: We keep getting a lot of paraffin on the floor of one histo room--especially around the microtome and the embedding station. Short of laying down a tarp, what do folks do keep wax off of the floor? Thanks, Jerry Ricks Research Scientist University of Washington Department of Pathology ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Submission of tonsils to pathology
We do micros on every one of them. On Jun 3, 2011, at 12:13 PM, Horn, Hazel V hor...@archildrens.org wrote: Generally all are gross only. There are some exceptions depending on patient history or if one is significantly larger than the other the pathologists have the discretion to do a microscopic. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's WaySlot 820 Little Rock, AR 72202 phone 501.364.4240 fax501.364.3155 visit us on the web at:www.archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, June 03, 2011 11:11 AM To: Histonet; Richard Cartun Subject: Re: [Histonet] Submission of tonsils to pathology Labelled gross only. René J. --- On Fri, 6/3/11, Richard Cartun rcar...@harthosp.org wrote: From: Richard Cartun rcar...@harthosp.org Subject: [Histonet] Submission of tonsils to pathology To: Histonet histonet@lists.utsouthwestern.edu Date: Friday, June 3, 2011, 11:55 AM What is your policy on the submission of tonsils to pathology for examination (gross or microscopic)? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: printed requisitions
We currently use paper reqs, but gasp coming soon, we will be going paperless. I am not sure I am going to like this... ;o). Surgical staff will enter the req in the computer, we receive the order the specimen and enter into our AP system. There are going to be growing pains!!! Sent from my iPhone On Jun 2, 2011, at 8:18 AM, Horn, Hazel V hor...@archildrens.org wrote: We still use paper reqs. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's WaySlot 820 Little Rock, AR 72202 phone 501.364.4240 fax501.364.3155 visit us on the web at:www.archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Wednesday, June 01, 2011 3:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] printed requisitions Does everyone still receive printed requisitions with your surgical specimens? How many of your OR's have a printer in each room? Our OR has a shared printer for all of the procedure rooms, but would like to have a printer for each OR suite. Our IT department is saying there would be problems and has suggested that we go paperless. I see many problems arising from this. If anyone is doing this successfully, I'd be very interested in hearing how you accomplished it. Toni Regards, Toni Rathborne Pathology Supervisor Somerset Medical Center 110 Rehill Ave. Somerville, NJ 08876 CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Keyboard and mouse covers
For the keyboard, what about using the silicon style? A lab near me uses them exclusively. They are easy to keep clean, as they basically watertight - just wipe down with a sani-wipe! And, they are fairly inexpensive - I got one from Office Max (or was it Staples?) for $10. Sent from my iPhone On Jun 1, 2011, at 1:21 PM, Weems, Joyce jwe...@sjha.org wrote: Actually, we put the mouse in a baggie. But the whole thing is covered. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: Rathborne, Toni [mailto:trathbo...@somerset-healthcare.com] Sent: Wednesday, June 01, 2011 13:19 To: Weems, Joyce; Dessoye, Michael J; histonet@lists.utsouthwestern.edu Subject: RE: Keyboard and mouse covers Do you completely wrap your mouse? Or do you leave the ball exposed? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, June 01, 2011 1:16 PM To: Dessoye, Michael J; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Keyboard and mouse covers We use plastic wrap... Easy to change out and keep clean! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J Sent: Wednesday, June 01, 2011 13:01 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Keyboard and mouse covers Hi, We're upgrading our grossing areas with new workstations that require the techs to interact with a keyboard and mouse. I'd like to get keyboard and mouse covers to protect them from the usual hazards of grossing! But I can't seem to find any big vendors that sell them...does anyone use them and could recommend a vendor for these covers? Thanks! Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdess...@wvhcs.org mailto:mjdess...@wvhcs.org | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain
Re: [Histonet] hematoxylin washed off
We had a similar thing happen last week, except in our case it was the eosin was very pale the hematoxylin was more blue than purple. Through our troubleshooting steps, we determined it could have only been the processor reagents. We fully changed it and the problem went away. While I am delighted that the staining has returned to normal, I have no idea what really happened. Here are some of my first thoughts: Have you checked your reagents on the stainer? Same lot numbers, differerent lot number? Do you use progressive or regressive technique? If regressive, are your differentiating/bluing reagents new since the processor change? Did you use new/different reagents on the processor? Did you wash the bottles/dishes with a cleaning solution that, maybe, didn't get fully rinsed out? Or do the dishes need stronger cleaning? Michelle Sent from my iPhone On May 7, 2011, at 2:42 AM, V. Neubert histonet.nos...@vneubert.com wrote: Is it only the HTX which is pale? Did you change anything else? Like dewaxing? anuradha shrivastava: Hello Every body, We changed the solutions testerday in the processor, and today Dr. complained about pale hematoxylin. Can u suggest what is wrong. thanks. anu. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Florida Law Question
Hi Histonetters! Can anyone tell me (maybe even provide a req) as to whether or not a lab aide can run the Histos 5 microwave tissue processor? It's semi-automatic, which involves the person (lab aide, tech etc) moving a carousel of cassettes from one beaker to another. Any help is greatly appreciated! Michelle ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] April 1st Fun
We had a patient we named Oscar Meyer... it was a piece of hot dog that we put in formalin, the doc grossed it, we cut it (and it cut very well, I might add!) and they got to the point of looking under the scope before they realized the joke! ;o) Michelle On Mar 29, 2011, at 2:13 PM, Breeden, Sara sbree...@nmda.nmsu.edu wrote: Dead cockroach on autopsy/necropsy table as legitimate specimen; goldfish processed as patient Barry Cuda; change family photos to total strangers; butterscotch pudding in (unused) specimen jar and subsequent tasting with applicator stick; activated charcoal on oculars (a la Colonel Potter in MASH); single hair attached to ocular with super-glue (tickling nose); cut fingertips off gloves; pumpkin/squash in animal shapes to slide; bug in block; foam-rubber-stuffed-box-with-toy-rat-to-burst-out-and-cause-startlement (toy rat to morph into histo lab mascot complete with appropriate holiday costume); process beef jerky as legitimate specimen; light coating of oil on 'scope oculars; - and the latest one I just received - a small slip of paper on the underside of the (computer's) mouse. Oh - the joy! What shall I do first?! Happy Annoying! This ought to cover the last several months I've not done the Friday Hour of Fuming... Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Bone marrow charges
It is my understanding that you may now charge for every stain, even if it's two blocks from one bottle. The CMS requirements changed in Oct of 2009, if memory serves correctly. Perhaps some one else can site the reference or go back into the archives for it. I'm not at my computer, or I would do it myself! Sent from my iPhone On Feb 23, 2011, at 10:06 AM, Rathborne, Toni trathbo...@somerset-healthcare.com wrote: Are your blocks (1 bx and 1 clot) separate specimens, or just one specimen with 2 blocks? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]On Behalf Of Goodwin, Diana Sent: Monday, February 21, 2011 11:40 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Bone marrow charges Greetings, Histonetters. How many times can I charge 88313 for a bone marrow case that has an Iron stain on 2 separate blocks (one for the bx and one for the aspirate clot) and also on 1 smear made from the aspirate clot, a PAS on the 2 blocks, a Trichrome and a Retic on the bx. block, and a Wright/ Giemsa on 4 smears? Thank you!!! Diana Goodwin Supervisor, Histology Laboratory RWJUHHNJ Diana Goodwin Supervisor, Histology Laboratory xt. 6996 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Bacterial contamination
Another place to check is the holding water that the slides rest in just prior to staining. We run our slides to water on the HE stainer and then transfer the rack to a holding water dish. We bleach this dish nightly, as we have found it contaminated in the past. We don't know what caused the contamination, but we have not it since the bleaching started! ;o) Good luck! Michelle On Jan 27, 2011, at 1:41 PM, bsulli...@shorememorial.org wrote: One place you need to look is the floatation bath where you cut your slides. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Speak only well of people and you need never whisper Breeden, Sara sbree...@nmda.nm su.eduTo Sent by: Histonet histonet-bounces@ histonet@lists.utsouthwestern.edu lists.utsouthwest cc ern.edu Ragsdale, John jragsd...@nmda.nmsu.edu Subject 01/27/2011 01:39 [Histonet] Bacterial contamination PM My pathologist tells me I have floating bacteria in both special stains I did this morning (GMS and Gram); some slides have these floating critters and some don't. Because the only common solutions are those for processing and deparaffinization and because these bacteria appear to be floating above the plane of the tissue - I can't figure out where to start looking. My DI water is from a central source and is routinely quality-checked, and this is a new building (Sep. 2010) I don't want to blame that. Knowing full well that I am probably overlooking the obvious, I'm asking for help figuring this out. I need a Sputnik Moment. Thanks! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Leica autostainer XL
I have a run set up to bake the slides run them down to water ( for my specials), another just to start at hematoxylin down to xylene etc. The stainer allows for nearly any custom program you can think of. :o) If you have any questions about how to set the program up, please let me know I will be happy to help you with it. Michelle On Jan 26, 2011, at 11:52 AM, Langenberg, Stacey stacey.langenb...@ucdenver.edu wrote: You can set up a just depar run. Sent from myTouch 4G - Reply message - From: Nicole Tatum nic...@dlcjax.com To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica autostainer XL Date: Wed, Jan 26, 2011 9:48 am Does anyone know if you can just deparaffinize slides in the stainer. Im just wondering if I also need a small stain line to dewax specials. Thank you, Nicole ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Lost Blocks
Our method isn't as technology savvy, but it works well for us. All blocks submitted for processing are written down on our piece sheet. At the end of the grossing day, two staff members compare the blocks in the processing basket to those listed on the piece sheet. At embedding, the histotech checks off the blocks prior to embedding. On Jan 7, 2011, at 3:25 PM, Patrick Laurie foreig...@gmail.com wrote: Here we take a picture of the blocks before they go on the processor. We have a barcoding system that lets me know who embedded what and when. With over 140,000 blocks/year we only have had one instance where a block was lost between grossing and embedding. Because we have some off-site pathologists and grossing staff, our lab relies very heavily on tracking every specimen that comes in, every block that goes to histology and every stained slide that goes to our pathologists. Good luck, it can be frustrating when you lose a block. On Fri, Jan 7, 2011 at 9:45 AM, Paula Wilder hist...@hotmail.com wrote: HI all, Does anyone have a procedure outlining checks and balances to assure blocks have been placed on the processor, blocks that are missing, either before or after embedding, etc. Any help would be greatly appreciated! Thank you, Paula Wilder St. Joseph Medical Center Towson, MD 21204 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plau...@cellnetix.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Stupid, stupid static!!
I have a tech who complains about static/compression in our cell blocks, but I don't seem to have the same problem as her. The difference? I face my blocks and then soak it on my waterbath for about 15 -30 seconds and then it goes onto the ice tray. The additional moisture that is soaked up in the block seems to correct the issue for me. Sort of like breathing heavily on the block! Michelle On Jan 6, 2011, at 4:36 PM, Johnson, Nacaela nacaela.john...@usoncology.com wrote: I am also having issues with static, but only on my particle blocks. This happens in the summer as well. The static is actually in the block. The core biopsies cut wonderfully. Does anyone else run into this problem? I can see the effects of the static when I'm embedding, the particles float to the edges of the mold instead of in a clump. Thanks, Nacaela Johnson Histology Technician KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email: nacaela.john...@usoncology.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sgoe...@mirnarx.com Sent: Wednesday, January 05, 2011 1:15 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Stupid, stupid static!! So where the microtome is here that I have to use we have to wear those blue hospital booties and disposable lab coats (the white paper type ones). With me and several other people walking around in those booties the amount of static electricity is to say the least frusterating!! Does anyone know of anything I can do to get rid of the static? Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet /preThe contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.brOnly the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone./pre ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] No Mail??
Oh man! The first time Histonet stopped working, I thought it was my email... but this time I knew better! And I too didn't realize how much I looked forward to the messages/thoughts/questions shared on this board. So histonet, what's been causing the problem? Michelle On Dec 17, 2010, at 11:29 AM, Harrison, Sandra C. sandra.harris...@va.gov wrote: Wow...didn't realize how addicted to Histonet I'd become till this week! Sandy Harrison Histology Supervisor VA Medical Center Minneapolis, MN 55417 612-467-2449 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Friday, December 17, 2010 9:54 AM To: Behnaz Sohrab; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] No Mail?? And I kept getting a returned msg yesterday that I was trying to post. I am experiencing withdrawal!!! :)j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Behnaz Sohrab Sent: Friday, December 17, 2010 10:48 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] No Mail?? Any Problem? I have not received any email from Histo-net last few days ?? Behnaz ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cardboard slide flat storage
Hi Meghan, We use grey colored boxes that will hold either 8 cardboard drawers of blocks or 4 cardboard drawers of slides. I am at home right now, don't have the specific ordering information, but will share it tomorrow when I get to work. I just didn't want to let the question languish w/o some sort of response! Michelle On Nov 15, 2010, at 9:30 AM, Meghan Tucker meghan.tuc...@yahoo.com wrote: Good morning, Are there any suggestions on a place to order a storage shelf for cardboard slide flats, which may have 6-8 'cubbies' that can be used to organize the flats? Thanks! Meghan meghan.tuc...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Formalin
Not in Florida either. We use Aldex to neutralize the formalin then dispose of it on the biohazard trash. The mfr says once neutralized you can put it in regular trash, but we don't. Michelle On Nov 11, 2010, at 7:45 PM, jmacdon...@mtsac.edu wrote: Absolutely not here in southern California. Sent via BlackBerry by ATT -Original Message- From: godsgal...@aol.com godsgal...@aol.com Sender: histonet-boun...@lists.utsouthwestern.edu Date: Thu, 11 Nov 2010 17:44:52 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin Ok, so how many of you are allowed to dump formalin down the drain? Sent from my Verizon Wireless Phone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Re: Frozen Section TAT
We have a calendar book in the frozen section room. The pathologist places the patient label on the book on the appropriate date, writes both the start stop times. I don't count the multiple block ones, but I don't tell them that! ;o) If they stay in the habit, all the better for me! Oh yeah, we have a large clock on the wall, easy to find, see and read! Michelle On Nov 2, 2010, at 5:46 PM, Robert Richmond rsrichm...@gmail.com wrote: Some comments on this dismal piece of busy work: It helps to have a time stamper so the person receiving the specimen can stamp the time on the requisition. A work sheet for each frozen section case makes it easier for the pathologist to record the time the report is telephoned, the frozen section diagnosis, and other useful information. A clock by the frozen section microscope is both a reminder and a hint, particularly for those of us who don't wear wrist watches in the lab. Turning a single block case around in 20 minutes isn't very difficult. The usual cause of delay is a difficult diagnosis where more than one pathologist looks at the slides. There is no TAT requirement for multiple block cases. Pathologist compliance is a major issue. Some pathologists entirely refuse to record TAT. Frozen sections are the highest stress area of most pathologists' practice, and it's easy to forget procedural details, particularly this one, which is of no benefit to the patient, nor to anyone else except the paper-pushers. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Frozen Section TAT
Allison, I have created an excel spreadsheet where you put time started, time completed it will calculate the amount time spent doing the frozen. It highlights any time grater than 20 min. Let me know if you are interested in something like that. Michelle On Nov 1, 2010, at 2:54 PM, Scott, Allison D allison_sc...@hchd.tmc.edu wrote: Hello to all in histoland. How is everyone calculating the FS TAT. Does any anyone have a file that automatically calculates that tat when you plug in the data, that they would be willing to share. Any help would be appreciated. Allison Scott Histology Supervisor LBJ Hospital. CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] eosin
It sounds like I am in the minority in pitting the eosin in the *first* 100% alcohol! I agree with Bill, it really makes a HUGE difference in small, minute tissues. We did run into one issue though, we were using orange biopsy cassettes and the red-orange tissue was difficult to spot in the orange cassettes! We have since switched to an aqua color and no more hiding specimens! ;o) Michelle On Oct 22, 2010, at 10:51 AM, Tench, Bill bill.te...@pph.org wrote: I think the use in a processor becomes a local culture. I think everyone in my community does it (someone spread the word), so now we all do. I believe that we are all putting it in the last alcohol. It really makes facing a block on minute pieces a whole lot easier. We found marking the tissue directly tedious, and it didn't persist as well in the processing. Because there is often so little visible material in our FNA cell blocks, we mark the histogel pellet with Davidson ink, and when the tech has just faced off the ink, he/she knows its time to start collecting sections. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 bill.te...@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations - Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Eosin to dye small Biopsies
We use about 40ml of eosin in the first 100% alcohol in both of our large specimen small biopsy machines. Michelle On Oct 21, 2010, at 4:33 PM, Scott, Allison D allison_sc...@hchd.tmc.edu wrote: Hello to all in histoland. Are any of you using eosin on the processor to dye your small bx's? If so, are you putting it in the 100% alcohol to do so? Any help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cassette Marking
Statlab pens for us. We have a cassette printer, but we hand write our slides. I *love* the statlab pens!! Sent from my iPod On Oct 19, 2010, at 5:26 PM, Sherwood, Margaret msherw...@partners.org wrote: Victor, We are a research lab and don't generate near the amount of specimens that a clinical lab would. We were happy to get an automatic stainer and coverslipper! Peggy -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Victor Tobias Sent: Tuesday, October 19, 2010 4:57 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Cassette Marking I'm somewhat surprised that many labs are still handwriting blocks and slides. If you are using a LIS, can it integrate printing blocks and slides? Is the cost too high to add the printing capability? The cost of equipment is so cheap compared with one lawsuit. It would also reduce the stress of loosing your job over a labeling mistake. Just seems like a win win for everyone. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 vic...@pathology.washington.edu 206-598-2792 206-598-7659 Fax = Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 10/19/2010 1:38 PM, Sean McBride wrote: Nita, We use HistoTec pens by Newcomer Supply ~Sean On Oct 19, 2010, at 2:22 PM, Nita Searcy wrote: ** High Priority ** If you HAVE to manually mark cassettes - what are you using? Cassette pens ? Pencils ? What is the rest of the world doing? Anything else on the market? Thanks Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsea...@swmail.sw.org 254-724-2438 Nita Searcy.vcf___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Re: Histonet Digest, Vol 83, Issue 20
Can you please provide the specific CMS update number? The website doesn't seem to be too user friendly ... thanks! On Oct 14, 2010, at 12:22 PM, Weems, Joyce jwe...@sjha.org wrote: CMS/NCCI Update Dated October 1, 2009 8. The unit of service for special stains (CPT codes 88312-88313) and immunohistochemistry (CPT codes 88342, 88360, 88361) is each stain. If it is medically reasonable and necessary to perform the same stain on more than one specimen or more than one block of tissue from the same specimen, additional units of service may be reported for the additional specimen(s) or block(s). Physicians should not report more than one unit of service for a stain performed on a single tissue block. For example it is common practice to cut multiple levels from a tissue block and stain each level with the same stain. The multiple levels from the same block of tissue stained with the same stain should not be reported as additional units of service. Only one unit of service should be reported for the stain on multiple levels from the single tissue block. Additionally, controls performed with special stains should not be reported as separate units of service for the stain. -Original Message- From: Mike Pence [mailto:mpe...@grhs.net] Sent: Thursday, October 14, 2010 11:31 To: Weems, Joyce; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 Can you site your source, please. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Thursday, October 14, 2010 10:25 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 The change is that you can bill per block now and not per specimen. This is for immunos and special stains. It does make a huge difference! Best, Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Chris Evanish Sent: Thursday, October 14, 2010 11:10 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 Has anyone heard of a cpt coding change that allows us to bill 88342 per slide run instead of per antibody? One of our Pathologist was at a conference and was told that we could do that. It makes a big difference with running cytokeratins on multiple blocks and levels of sentinel nodes. Thanks, Chris Evanish Montgomery Hospital Norristown PA Chris D. Evanish Histology Supervisor Montgomery Hospital 610-270-2379 Please consider the environment before printing this email to your outgoing mail. Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] (no subject)
Sean, what state are you in? If FL, there is a company called Southern Biomed who could probably help you out. Michelle On Sep 29, 2010, at 10:28 PM, Sean McBride smcbr...@andrew.cmu.edu wrote: Hello everyone, I have an old Leica RM 2065 microtome that is in need of repair (the clutch stopped working), but according to Leica, the machine is no longer supported by the company. Does anyone have any suggestions for a company or technician who might be able to repair the machine? Thanks in advance, ~Sean ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Ventana vs Leica
Unless someone corrects me (or even agrees with me!) in FL only a technologist is allowed to load the IHC machine, so no additional lean for us. :o( I would be interested to hear more about the savings though, as we are preparing to be in the market for a new IHC machine. We currently have the Benchmark XT. On Aug 26, 2010, at 10:08 AM, Mahoney,Janice A janice.maho...@alegent.org wrote: We love our Ventana instruments too Jay. I don't quite believe the 40% difference in cost. I'd like to see those numbers. I know I save in tech time and the instruments are very easy to use. We have histo assistants and secretaries trained to load and unload the instruments, saving out techs to do the things only techs can do. Talk about LEAN! A little more from a LEAN perspective, the Ultra is the only instrument out there that is TRUE continuous flow. As soon as there is an open spot on the instrument and the antibody is on board, I can add a slide. I don't have to wait till one of the 10 slide modules is finished. Leica is still a batch instrument, it is just smaller batches than the older IHC models. I'm not putting Leica down, it is a fine instrument but I think it is important for people to know the facts. Jan Mahoney Omaha -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of bsulli...@shorememorial.org Sent: Tuesday, August 24, 2010 2:05 PM To: Jay Lundgren Cc: histonet; histonet-boun...@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana vs Leica Jay, I currently use the Ventana and am very pleased with the results I get. The only draw back is the cost to run the instrument. It can get quite pricey. They added space on the antibody wheel but took space away from the slide area. This has impacted our work flow greatly. We are however looking to purchase a second one. This one will have continual through put. That should help out with TAT. Hope this helps. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Jay Lundgren jaylundg...@gmai l.com To Sent by: histonet histonet-bounces@ histonet@lists.utsouthwestern.edu lists.utsouthwest cc ern.edu Subject [Histonet] Ventana vs Leica 08/24/2010 02:30 PM I was wondering if anyone out there had experience with both the Ventana Ultra and the Leica Bond immunostainers. I realize that most people have a personal preference as to brands, but I'm not looking for a knee-jerk opinion (LEICA RULZ11 or VENTANA FTW!!), just someone who has had actual experience working on a daily basis with both instruments. If this is you, could you please tell me which you preferred and why. I'm currently working for a facility in MT which has narrowed down its search to these two instruments. No vendors please, they've already given their pitches. Thanks, Jay A. Lundgren M.S., HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list
Re: [Histonet] HE stainers
We have the new version of the Leica XL stainer (looks just like the old one!) with the bridge-transfer station coverslipper love it. We had the old stainer for 10 years before getting the new one... and even managed to sell the old one! Michelle On Jun 30, 2010, at 9:57 AM, Webb, Dorothy L dorothy.l.w...@healthpartners.com wrote: We are in the market for a new HE automated stainer and am hoping I get some feedback on this request! I wold like to hear from the techs who work with either the Prisma from Sakura or the Leica auto or multi stainers, PLEASE!! You can email me directly if you would like your comments private at dorothy.l.w...@healthpartners.com Thanks much ahead of time!! This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] toe nails falling off slides - Email found in subject
We have very good success in facing the block and soaking it on the water bath for 30-60 seconds and then allowing it to cool down on a very wet ice tray. Ours cut like butter too! :o) On Apr 28, 2010, at 8:58 AM, Cazares, Ruth rcaza...@schosp.org wrote: Or you could soak them in 10% sodium hydroxide for 20-30 minutes prior to processing. My techs say they cut like butter! Ruth Cazares, HT (ASCP) Histology Supervisor Department of Pathology Swedish Covenant Hospital 5145 North California Ave Chicago, IL 60625 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet- boun...@lists.utsouthwestern.edu] On Behalf Of Maria Katleba Sent: Tuesday, April 27, 2010 5:22 PM To: zodia...@comcast.net; histonet@lists.utsouthwestern.edu Subject: [SPAM-HC] - RE: [Histonet] toe nails falling off slides - Email found in subject HOW TO CUT NAILS (or other difficult tissue) SO THAT THEY STAY on slide! (1) Cut the slides and place on Plus(+) slide (2) shake off excess water (3) place slide immediately in plastic Coplin jar with 1-3 drops of formalin at bottom. (4) place in hot oven for 5 mins (50-60 degrees) (5) remove from Coplin jar under a hood as formalin drops are now carcinogenic vapour (6) place slides in rack in oven so that the formalin moisture dries... (7) slide is ready for use Maria Katleba HT(ASCP), MS Pathology Dept. Mgr. Queen of the Valley Medical Center 707-294-9229 cell 707-252-4411 x3689 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet- boun...@lists.utsouthwestern.edu] On Behalf Of zodia...@comcast.net Sent: Tuesday, April 27, 2010 12:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] toe nails falling off slides To all, I was wondering if anyone out there had any tips on how to keep toenail sections from falling off slides. We mount them on positive slides, air dry for about an hour but they still fall off. Any insight on the subject would be greatly appreciated. Thanks, Jenny ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Bone Marrow Clots - falling off slides
Thanks for all the input. I will check the collection method. B-plus is the fixative we normally use but I am not convinced that this clot ended up in it! Other tissues are not being dramatically affected and not even all the bone marrows! We do soak the blocks too. This is very frustrating. :o( Michelle On Apr 27, 2010, at 7:59 AM, Lynette Pavelich lpave...@hurleymc.com wrote: In my experience, it can be all of the above that can cause it. By the tissue not getting enough penetration by any of the solutions will alter the outcome. That is why I asked about the thickness (remember...only as thick as a nickel). Blood is also so dense, so it would really apply here. Try that. Do 1 change at a time. I've been in your shoes, it is very frustrating. Then if the thickness is good, work on something else.starting with fixation times. histot...@imagesbyhopper.com 4/27/2010 7:32 AM Mary, Thanks for the suggestion. We do check the alcohols with a hydrometer and if not 100%, we will use that alcohol as the base for dilution alcohols. We check quality of the xylene by putting 10ml of water in a graduated cylinder and then add 10ml of the recycled xylene to it and shake it vigorosly. Good, clean xylene should have a perfect separation and no mixing with the water. Lynette, I am leaning towards the processing too, but which part?? The clot seems to be more dry than moist, so it's not under dehydrated. Fixation? It spends 3 hours in formalin on the tissue processor and whatever time it can be in formalin prior to the processor. Paraffin impregnation? About the only thing I could think of here was if the wax wasn't changed fast enough and/or we got too much carry over from the xylene. Could that cause this? Thanks for the help! Michelle -Original Message- From: Lynette Pavelich [mailto:lpave...@hurleymc.com] Sent: Tuesday, April 27, 2010 6:56 AM To: histot...@imagesbyhopper.com; histonet@lists.utsouthwestern.edu; MARY HODGES Subject: RE: [Histonet] Bone Marrow Clots - falling off slides When I start losing tissue on the slide, I have found that it is usually a processing issue. Is the clot too thick? Try cutting them in half before processing. Hope this helped, Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: lpave...@hurleymc.com ph: 810-257-9948 Lab: 810-257-9138 fax: 810-762-7082 MARY T HODGES hodges...@msn.com 4/27/2010 12:18 AM recycled anything is not back to it original state always use a hydo meter and add new to back product of a better quality you will find this also with alcohols From: histot...@imagesbyhopper.com To: histonet@lists.utsouthwestern.edu Date: Mon, 26 Apr 2010 23:26:43 -0400 Subject: [Histonet] Bone Marrow Clots - falling off slides Hi Histonetters! We have started to have an issue with our bone marrow clots falling off the slides. We are using plus slides, making sure they drain well (just like our other slides), but when we stain them routinely, we are getting a fair amount of tissue coming off the slides. It has been suggested that it's related to our using recycled xylene. Does anyone have any experience with recycled xylene and this type of tissue fall-off? Another suggestion was that the tissues sat in xylene too long. I don't see how that could happen, under routine conditions, but is that a possibility for causing this? All thoughts will be appreciated! Michelle ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ Hotmail has tools for the New Busy. Search, chat and e-mail from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID28326::T:WLMTAGL:ON:W L:en-US:WM_HMP:042010_1___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.437 / Virus Database: 271.1.1/2835 - Release Date: 04/25/10 18:31:00 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
