Hello All I am a clinical researcher so my questions may be somewhat naive, but I would be extremely grateful for any help. I want to look at adipose tissue macrophage polarity by immunohistochemistry for activation markers. I have the following questions: 1. How much tissue sample is required. I want to use subcutaneous and omental fat samples. 2. How should I transport and store my samples. How long can I store them? I anticipate that I would be collecting samples over 2-3 months. 3. If I do H&E staining first, can I do immunohistochemistry on the same slide? Can I do successive markers on the same slide? How many markers can I use it for? Thanks Khadija _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
