Re: [Histonet] Reprocess Procedure
Thank you for your input. I would like to know how others are handling reprocess. Kiran Sent from my Verizon Wireless BlackBerry -Original Message- From: Grantham, Andrea L - (algranth) algra...@email.arizona.edu Sender: histonet-boun...@lists.utsouthwestern.edu Date: Mon, 15 Oct 2012 15:28:21 Cc: histonet@lists.utsouthwestern.eduhistonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Reprocess Procedure A few years ago this subject was discussed on histonet and somebody - I think it was Joe the Toe - said that they just melted the paraffin away from the tissue if it had been embedded and threw it back in to process with the next batch of tissues. The theory being that the part of the tissue that was processed ok was protected by the paraffin and the part that needed additional processing was exposed. I don't have to reprocess often but when I was gone a student had a problem with the processor and put in an emergency call to me. I told her to do this and the tissues came out fine the next day. Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edumailto:algra...@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: cost/slide
Hi, I am also interested in finding average cost of he , ihc and special stains in automated lab setting. Please send response to me directly. Thank you, Kiran Sent from my Verizon Wireless BlackBerry -Original Message- From: D'Attilio, Shelley sdatt...@stormontvail.org Sender: histonet-boun...@lists.utsouthwestern.edu Date: Thu, 20 Sep 2012 12:59:42 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Gastro prices Hi all, Lin asked about charges for HE slides, unstained slides, etc. for gastric biopsy. Lin, I just want to caution you about asking for this information on an open forum such as this. I'm not up on all the legal issues, but I think you want to avoid any suspicion of price fixing. A source of information might be the reference lab with whom you already do busines. And of course, take into consideration the amount of reimbursement for the test. Thanks, Shelley D'Attilio MT(ASCP) Manager, Chemistry, Cytology and Histology Dept. of Pathology and Laboratory Medicine Stormont-Vail HealthCare Topeka, Kansas NEED A DOCTOR? Stormont-Vail's Health Connections can help you find a doctor accepting new patients. Call (785) 354-5225. ** The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information. Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited. If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address. Thank you for your cooperation. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] burned tissue artifact: need help
Hi all, Due to water contamination on the processor some of our cases had processing artifact with poor histology. Any suggestion to remedy this issue? We did reprocess the blocks but still not good. Any cutting or staining tips so slides can be readable. Thank you all, Kiran Sent from my Verizon Wireless BlackBerry -Original Message- From: Amos Brooks amosbro...@gmail.com Sender: histonet-boun...@lists.utsouthwestern.edu Date: Thu, 20 Sep 2012 17:54:04 To: histonet@lists.utsouthwestern.eduhistonet@lists.utsouthwestern.edu Subject: [Histonet] Picric acid Hi, The Halifax explosion was indeed a very dramatic event. Anyone unfamiliar with the story should certainly read up on it. It was truly incredible. The link to the Wikipedia article was previously posted. A couple of important points about this story. The ship that blew up was carrying metric *tons* of picric acid. The ship also was carrying tons of other explosive material (nitroglycerine amongst others). If your lab has tons of picric acid (not 10-100 grams like most labs) and tons of other explosives, you might have cause for panic. If not, you likely have an extremely small amount stored under water or in a solution which poses less risk when used stored properly than many other chemicals in a lab. I don't mean to say there is no risk, but I would say the concern is a bit overly dramatic. Like a carpenter, know your tools and how to treat them and they will serve you well. Otherwise ANY of the tools you have are likely to bite you. Amos ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Peloris Rapid tissue processor
Hi We are currently using Peloris for most of our tissues and its great. Our goal is to move all processing from conventional VIP to Peloris by the end of this year. We validated our own cycle times, not factory installed. The key is size of the tissue and cycle time, if tissues are mixed and cycle time is not sufficient to process, it will cause problems. Thank you, Rene for bringing some good points especially evaporation step, I would definitely look into that. No issues so far and great service! Kiran Kaiser Permanente, CA Sent from my Verizon Wireless BlackBerry -Original Message- From: Rene J Buesa rjbu...@yahoo.com Sender: histonet-boun...@lists.utsouthwestern.edu Date: Sat, 13 Aug 2011 07:19:34 To: histonet@lists.utsouthwestern.edu; tahs...@brain.net.pk Cc: histol...@skm.org.pk Subject: Re: [Histonet] Peloris Rapid tissue processor Muhammad: The only thing I dislike about the Peloris technology (it is a technology in itself) is that after the dehydration with 2-propanol, the tissues are subjected to a DRY HOT evaporation of the 2-propanol in vacuum before the infiltration step with melted paraffin. That step of drying out the tissues to eliminate the 2-propanol to facilitate the infiltration is the one I do not like because the tissues are exposed to a very high gradient. If you end buying the instrument I think you should run a large series of validation tests to find out if the results you obtain with the Peloris compare with what you are used to, not referring to the sectioning quality of the blocks, but to their microscopic appearance.. Peloris was developed in Australia by VisionBioSystems and later bought by Leica Microsystems. Although there were tests published by VisionBioSystems, all referred to animal tissues, and the instrument was never independently validated. Leica did not make known (published) independent validations. I for one would never subject the tissues to a hot dry desiccation before infiltration. I hope this will help you in your decision. René J. --- On Sat, 8/13/11, tahs...@brain.net.pk tahs...@brain.net.pk wrote: From: tahs...@brain.net.pk tahs...@brain.net.pk Subject: [Histonet] Peloris Rapid tissue processor To: histonet@lists.utsouthwestern.edu Cc: histol...@skm.org.pk Date: Saturday, August 13, 2011, 6:18 AM Dear All, Our lab is in the process of purchasing our automated tissue processor (Peloris Rapid tissue processor). I would really appreciate comments from anyone who really likes, or dislikes the processor that they are using. Thanks in advance!!! Muhammad Tahseen Histology Supervisor Deptt. Pathology Shaukat Khanum Cancer Hospital And Research Center Lahore. Pakistan Ph. +92 42 5180725-36 Ext 2369, Fax. +92 42 5180723 e-mail. Histology @t skm.org.pk ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] TMA instrument
Need help to find best TMA instrument available in the histo world. Any feedback or recommendations are welcome. Thank you, Kiran Sent from my Verizon Wireless BlackBerry ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] No patient ID: Ink dissolved from Cassettes duringprocessing.
Thank you all for excellent input and sharing your experience. Hope this doesn't happen again! - Kiranjit Sent from my Verizon Wireless BlackBerry -Original Message- From: Margaret Blount ma...@medschl.cam.ac.uk Date: Tue, 3 May 2011 08:52:59 To: Kiranjit Grewalkira...@sbcglobal.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] No patient ID: Ink dissolved from Cassettes duringprocessing. Pencil does not dissolve in processing reagents and is safe. I have also found the marker pens from Surgipath to be the best of those I have tried, but with the proviso that the labels should be left to dry for a couple of hours before being immersed in ethanol. I routinely use one of these pens for labelling my slides. The old fashioned way was to put a labelled slip of card or paper into the cassette with the tissue. I don't know how this would fit in with US regulations... Margaret -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kiranjit Grewal Sent: 29 April 2011 22:44 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] No patient ID: Ink dissolved from Cassettes duringprocessing. Hi All, What is the standard practice out in histology world if hand written cassette id washed away during processing? Please share if you had any experience and how did you resolve this and what is your current practice. Thank you so much! -Kiranjit ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet]Need input: Ink dissolved from Cassettes during processing.
Thank you! Does anybody take a digital photo to correlate cassettes when using hand written cassettes as a back up? Or Do you put a piece of paper inside the cassette as back up for hand written cassettes? Need input so we can prevent future incidents. Thx Kiranjit Sent from my Verizon Wireless BlackBerry -Original Message- From: Rene J Buesa rjbu...@yahoo.com Date: Sat, 30 Apr 2011 07:39:14 To: histonet@lists.utsouthwestern.edu; Kiranjit Grewalkira...@sbcglobal.net Subject: Re: [Histonet] No patient ID: Ink dissolved from Cassettes during processing. Eleven years ago that happened in our laboratory in what we started to call The Black Tuesday (it was from Monday to Tuesday). Thanks that we always kept all cassettes described-written in a log with the type of tissue and the number of pieces with their sizes. Then we went through the list of cassettes that were stored in the baskets in sequential order. That, and the cases description, allowed us to identify all the 268 cassettes. We also informed the chief pathologist and documented it in our QC. The HT that decided to use a different pencil to write the cassettes was counseled. We also instituted a check in of the pencil before writing the daily cassette load. I sympathize with your issue, it was really a nightmare in our lab that we were fortunate enough to overcome (thanks to our chain of custody procedure). René J. --- On Fri, 4/29/11, Kiranjit Grewal kira...@sbcglobal.net wrote: From: Kiranjit Grewal kira...@sbcglobal.net Subject: [Histonet] No patient ID: Ink dissolved from Cassettes during processing. To: histonet@lists.utsouthwestern.edu Date: Friday, April 29, 2011, 5:43 PM Hi All, What is the standard practice out in histology world if hand written cassette id washed away during processing? Please share if you had any experience and how did you resolve this and what is your current practice. Thank you so much! -Kiranjit ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
