[Histonet] Orienting GI biopsies during embedding
Everyone, We are having a heck of a time orienting GI biopsies but esophageal biopsies in particular, during embedding. If anyone could share their secret for getting these tiny specks of semi-transparent tissue oriented, we would greatly appreciate it. Sabina Sylvest Department of Pathology Cincinnati Children's Hospital ssylv...@cinci.rr.com (513)803-0741 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Procedure for anti-human TCR V alpha24
Hello all, I am working in an Allergy and Immunology research lab and we have been trying to work up a flow cytometry antibody for IHC on paraffin. The antibody is mouse anti-human TCR V alpha24-Biotin from BeckmanCoulter. Does anyone have a working procedure for this antibody? I have tried many dilutions, retrieval methods and incubation times but nothing seems to work. Thanks in advance for your help. Sabina Sylvest CLS II Department of Allergy and Immunology Cincinnati Children's Research Foundation Cincinnati, OH (513)803-0741 ssylv...@cinci.rr.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
