[Histonet] Equipment maintenance
To All, I just wanted to see what companies other labs are using for the maintenance of their equipment(processor, embedder, microtomes...). We are located in south jersey. Thanks for your help Jenny ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] positive slides
Hello, Can anyone recommend a brand of positive slides that are good for IHC. We are having problems with tissue falling off of the slides. Thank You Jenny ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] section shrinkage during IHC
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[Histonet] shrinkage during IHC
To All, We are a lab that sends our specimens out for IHC and have just switched to another reference laboratory for these services. Our pathologist is saying that the tissue looks shrunk on the IHC slides, yet the slides that I process (HE, and special stains)are fine. Does anyone know what is causing this? The reference lab said it could be the type of slides that I use to mount the sections we send to them. My knowledge in IHC is limited. Also, if this helps, they are FFPE tissue. Thanks for your help Jenny ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Paraffin
Hello, Just wanted to know if anyone has tried Henry's Wonder Wax Xtreme Paraffin w/DMSO made by Mercedes Medical. What were your expirences with it? Is it comprable to McCormick's Paraplast Xtra? Thanks in advance, Jenny ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] filing unstained slides
To All, Just wanted to know how other labs are filing their unstained slides?? If filing along with stained coverslipped slides wouldn't the sections on the unstained slides get scratched?? Any input is appreciated. Thanks in advance, Jenny ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] dimethyl sulfoxide
To All, Does anyone know where to purchase Dimethyl Sulfoxide for KOH preperation? Thanks in advance, Jenny ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] toe nails falling off slides
To all, I was wondering if anyone out there had any tips on how to keep toenail sections from falling off slides. We mount them on positive slides, air dry for about an hour but they still fall off. Any insight on the subject would be greatly appreciated. Thanks, Jenny ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] thank you
Thank you to everyone who responded to my question regarding where to purchase Toluidine blue. It is very much appreciated. Jenny ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Toluidine blue
Hello to all, I was wondering if anyone knows where to purchase toluidine blue (for mast cells) other than polyscientific. Thank you in advance for your replies, Jenny ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HT exam
Hello to all, I am in the process of trying to study for the HT exam. I was wondering if anyone has any study aids they would be willing to share? I would greatly appreciate it. Right now I have F. Carson's book that I am reviewing. There is so much information to study, I'm not sure how to approach it. Any advice would be a great help. Thanks, Jenny ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] automatic coverslipper
Hello everyone, Out lab is looking to getting an automatic coverslipper, we do about 6000 cases a year. What would be a good recommendation? Thanks Jenny ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ci-Trol
Hello Everyone, Is anyone familiar with Ci-trol (citrated plasma) for making cell blocks? Someone had recommended a procedure using this, and I wanted to get some feedback on it. Thanks in advance! Jenny ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] fume exposure
To All, I work in a lab where I stain and coverslip everything by hand. I coverslip under a fume hood so there isn't much of an issue there. The main issue is my exposue to fumes while staining. I do not stain under a fume hood. My exposure times to these c hemicals is increasing becuase our volume is steadly increasing as well. I can no longer tolerate these fumes and am expirenc ing difficulty breathing and frequent headaches.What are labs that stain there slides by hand doing (types of masks, hoods) to minimize their exposure. If you can recommed any specifics I would appreciate it. Thank you in advance Jenny ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
