We use an Azure B counterstain. Melanin has a greenish tinge after counterstaining.
-----Original Message----- From: jayalakshmy p.s via Histonet <histonet@lists.utsouthwestern.edu> Sent: Monday, April 11, 2022 11:59 PM To: histonet@lists.utsouthwestern.edu Subject: [External] [Histonet] Clarification about Immunohistochemistry External: Be careful with links and files. If suspicious, alert the Information Security Office. Questions? Call 570-271-8092 Hai all Histonetters Please anybody clarify my this doubt if possible. When doing Immunohistochemistry for confirmation of Melanoma with DAB chromogen(brown color)the interpretation is not possible because of obscuring by the dense melanin pigment. We dont have any other color chromogen. I tried ihc after bleaching but the section gets detached even from charged slides. Is there any other effective way to do this? Thanks in advance Regards Dr. P S Jayalakshmy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=04%7C01%7Cakbitting%40geisinger.edu%7Ceafd323c5fa44fdf757908da1c38d5e6%7C37d46c567c664402a16055c2313b910d%7C0%7C0%7C637853327692391791%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000&sdata=JxGzP940ehU4hgYAjtwoKI5EaLl0CUFjbi7iAvahMYw%3D&reserved=0 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
