Thanks all,
This is more or less what i thought, that 20mu sections would prove to be
difficult and not all that feasible. So, I wait with bated breath for the
protocol from the researcher
On 3/13/09, Peggy Bisher mbis...@princeton.edu wrote:
One of the labs here use JB4 to section Zebrafish. I sent your question to
them to see if they could help you out. Here is their response:
The consensus in the lab (Kari and I) is that no, probably not. The size
would probably shred the section and chip it to where they would be uneven,
etc. Probably cryo or vibratome would be best.
They routinely cut their sections between 2-5 microns.
Good luck to you!
Cheers,
Margaret E. Bisher
Electron Microscopy Histology Core Facility Manager
Department of Molecular Biology
Princeton University
Moffett Laboratory, Room 113
Princeton, New Jersey
Office: (609) 258-7026
Fax: (609) 258-8468
mbis...@princeton.edu
On 3/13/09 3:53 AM, louise renton louise.ren...@gmail.com wrote:
Hi all,
I have a query from a colleague doing research on neuroanatomy as to
whether
it is possible ( with relative ease) to cut 20mu sections from JB4 resin
embedded tissue? Apparently these sections ae to be stained and then used
for stereomicroscopy. My experience is not that extensive to be able to
answer her, so I would appreciate some advice here
best regards--
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
There are nights when the wolves are silent and only the moon howls.
George Carlin
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However, many electrons were terribly inconvenienced.
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--
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
There are nights when the wolves are silent and only the moon howls.
George Carlin
No trees were killed in the sending of this message.
However, many electrons were terribly inconvenienced.
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