Re: [Histonet] A question for my hospital lab peeps
If a SLN is sent for frozen section (boo hiss) we treat as any other frozen and we typically cut two sections. If the pathologist is able to determine the lymph node is positive, we will only cut a permanent H&E once processed; we do not need to cut all the SLN levels. If the tissue appears negative on frozen then we will treat it as a routine SLN; we then cut the permanent H&E and an unstain, trim in about 100 um, cut an H&E and an unstain, trim in about 100 um and cut the final H&E. Hope this makes sense! Samantha Sent from my iPhone > On Feb 23, 2023, at 1:38 PM, Terri Braud via Histonet > wrote: > > I'd love to hear your input > On breast mastectomy cases with sentinel lymph nodes, what is your cutting > protocol for sentinel lymph nodes sent for frozen section? > How many frozen levels? > How many permanent section Levels? > Any unstained ffpe sections saved or stained IHC? > I'm just trying to make sure that what we're doing is the norm. Thanks in > advance. > > Terri L. Braud, HT(ASCP) > HNL Laboratories for > Holy Redeemer Hospital > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > Ph: 215-938-3689 > Fax: 215-938-2021 > Honesty > AccouNtability > AgiLity > CoLlaboration > CoMpassion > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] A question for my hospital lab peeps
Hi Terri! At the hospital I'm currently at, we do the same as Lacy's lab for permanent and frozen sections. We also wait for the pathologists to put in IHC requests before cutting them and they will also often request a follow up H&E. I have worked at another facility that would a 'level 1' and 'level 100' on all sentinel nodes without any IHC up front. Hope that helps! Amber Cone, HTL(CM) -Original Message- From: Normington, Lacy Sent: Thursday, February 23, 2023 12:56 PM To: Terri Braud ; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] A question for my hospital lab peeps Hello Terri, Please see an excerpt from our frozen section manual. For all sentinel lymph nodes and non-sentinel lymph nodes obtained from breast and gynecologic surgeries, entirely submit the lymph node after serially sectioning perpendicular to the long axis at 0.2 cm intervals. It is essential that all unique surfaces be represented, i.e. opposing surfaces should not be embedded in the same direction. How many frozen levels? Seeing as we are serially sectioning the lymph node, we only do a single level. How many permanent section Levels? All remnants are submitted after frozen, again we only perform the microtomy of a single level. Any unstained ffpe sections saved or stained IHC? It is not uncommon for AE1/AE3 to be requested which we then cut the IHC request followed by an additional recut H&E. Thanks Lacy Normington Lacy Normington, HTL(CM) She/Her/Hers Manager, Clinical Laboratories Surgical Pathology, Autopsy 600 Highland Ave, Mail 3224 Madison, WI 53792 Office: 608.890.9373 -Original Message- From: Terri Braud via Histonet Sent: Thursday, February 23, 2023 12:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] A question for my hospital lab peeps WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. I'd love to hear your input On breast mastectomy cases with sentinel lymph nodes, what is your cutting protocol for sentinel lymph nodes sent for frozen section? How many frozen levels? How many permanent section Levels? Any unstained ffpe sections saved or stained IHC? I'm just trying to make sure that what we're doing is the norm. Thanks in advance. Terri L. Braud, HT(ASCP) HNL Laboratories for Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3689 Fax: 215-938-2021 Honesty AccouNtability AgiLity CoLlaboration CoMpassion ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.com/v3/__https://nam04.safelinks.protection.outlook.com/?url=http*3A*2F*2Flists.utsouthwestern.edu*2Fmailman*2Flistinfo*2Fhistonet&data=05*7C01*7CLnormington*40uwhealth.org*7C39c802fc6114419b6f1508db15ca3212*7C0fd7902a3b4f49b0b1edaaa4d2b4f5f1*7C0*7C0*7C638127730396655742*7CUnknown*7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0*3D*7C3000*7C*7C*7C&sdata=NneLV1tWvVwR7RTukOLBHO4QRmy0EVgPKIg2OzIbAbs*3D&reserved=0__;JSUlJSUlJSUlJSUlJSUlJSUlJSUlJQ!!JA_k2roV-A!ASPwfj3ZVD46XC_NZmxt5qQW7S_nAt2r3ES2zSsZuduBtr8z1zfsGo8cwEqZpVQAdDa4HwlRdRUHMQLunLjs2K945QJbmSDSz3wvhPnitg$ ** The information contained in this e-mail may be privileged, confidential, and/or protected from disclosure. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly prohibited and no waiver of any attorney-client, work product, or other privilege is intended. No binding agreement on behalf of Baylor Scott & White Health, or any affiliated entity, is permitted by e-mail without express written confirmation by a duly authorized representative of Baylor Scott & White Health. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] A question for my hospital lab peeps
Hello Terri, Please see an excerpt from our frozen section manual. For all sentinel lymph nodes and non-sentinel lymph nodes obtained from breast and gynecologic surgeries, entirely submit the lymph node after serially sectioning perpendicular to the long axis at 0.2 cm intervals. It is essential that all unique surfaces be represented, i.e. opposing surfaces should not be embedded in the same direction. How many frozen levels? Seeing as we are serially sectioning the lymph node, we only do a single level. How many permanent section Levels? All remnants are submitted after frozen, again we only perform the microtomy of a single level. Any unstained ffpe sections saved or stained IHC? It is not uncommon for AE1/AE3 to be requested which we then cut the IHC request followed by an additional recut H&E. Thanks Lacy Normington Lacy Normington, HTL(CM) She/Her/Hers Manager, Clinical Laboratories Surgical Pathology, Autopsy 600 Highland Ave, Mail 3224 Madison, WI 53792 Office: 608.890.9373 -Original Message- From: Terri Braud via Histonet Sent: Thursday, February 23, 2023 12:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] A question for my hospital lab peeps WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. I'd love to hear your input On breast mastectomy cases with sentinel lymph nodes, what is your cutting protocol for sentinel lymph nodes sent for frozen section? How many frozen levels? How many permanent section Levels? Any unstained ffpe sections saved or stained IHC? I'm just trying to make sure that what we're doing is the norm. Thanks in advance. Terri L. Braud, HT(ASCP) HNL Laboratories for Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3689 Fax: 215-938-2021 Honesty AccouNtability AgiLity CoLlaboration CoMpassion ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://nam04.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C01%7CLnormington%40uwhealth.org%7C39c802fc6114419b6f1508db15ca3212%7C0fd7902a3b4f49b0b1edaaa4d2b4f5f1%7C0%7C0%7C638127730396655742%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=NneLV1tWvVwR7RTukOLBHO4QRmy0EVgPKIg2OzIbAbs%3D&reserved=0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] A question for my hospital lab peeps
I'd love to hear your input On breast mastectomy cases with sentinel lymph nodes, what is your cutting protocol for sentinel lymph nodes sent for frozen section? How many frozen levels? How many permanent section Levels? Any unstained ffpe sections saved or stained IHC? I'm just trying to make sure that what we're doing is the norm. Thanks in advance. Terri L. Braud, HT(ASCP) HNL Laboratories for Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3689 Fax: 215-938-2021 Honesty AccouNtability AgiLity CoLlaboration CoMpassion ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
