Re: [Histonet] AFB STAIN
I completely agree with Tony. Not likely to see any sloughing off (shedding) of organisms from FFPE tissue blocks. William DeSalvo From: Tony Henwood (SCHN) via Histonet Sent: Thursday, October 4, 2018 1:54 PM To: adesupo2...@hotmail.com; Laurie Colbert Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] AFB STAIN Cross-contamination has not been proven in FFPE tissues. I have not seen it in nearly 40 years of practice Do you have any evidence of cross-contamination in FFPE? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children’s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA From: Laurie Colbert via Histonet Sent: Thursday, October 4, 2018 10:36 PM To: adesupo2...@hotmail.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] AFB STAIN You should put the AFB control on a separate slide to prevent cross-contamination. Laurie Redmond -Original Message- From: ADESUPO ADESUYI via Histonet To: histonet Sent: Wed, Oct 3, 2018 7:58 pm Subject: [Histonet] AFB STAIN Hello, I have a question please. For the AFB Stain, do we put both the control tissue and the patient on the same slide? Thanks, Ade ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://nam01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonetdata=02%7C01%7C%7Cfe0f25b67e0d4783c39008d62a3b84a1%7C84df9e7fe9f640afb435%7C1%7C0%7C636742832440684556sdata=WQS0%2F1lqe%2FRJ0mB6k4NNlqYdIbvQ5sPx2EVn213ittE%3Dreserved=0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://nam01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonetdata=02%7C01%7C%7Cfe0f25b67e0d4783c39008d62a3b84a1%7C84df9e7fe9f640afb435%7C1%7C0%7C636742832440684556sdata=WQS0%2F1lqe%2FRJ0mB6k4NNlqYdIbvQ5sPx2EVn213ittE%3Dreserved=0 This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://nam01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonetdata=02%7C01%7C%7Cfe0f25b67e0d4783c39008d62a3b84a1%7C84df9e7fe9f640afb435%7C1%7C0%7C636742832440684556sdata=WQS0%2F1lqe%2FRJ0mB6k4NNlqYdIbvQ5sPx2EVn213ittE%3Dreserved=0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] AFB STAIN
Cross-contamination has not been proven in FFPE tissues. I have not seen it in nearly 40 years of practice Do you have any evidence of cross-contamination in FFPE? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children’s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA From: Laurie Colbert via Histonet Sent: Thursday, October 4, 2018 10:36 PM To: adesupo2...@hotmail.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] AFB STAIN You should put the AFB control on a separate slide to prevent cross-contamination. Laurie Redmond -Original Message- From: ADESUPO ADESUYI via Histonet To: histonet Sent: Wed, Oct 3, 2018 7:58 pm Subject: [Histonet] AFB STAIN Hello, I have a question please. For the AFB Stain, do we put both the control tissue and the patient on the same slide? Thanks, Ade ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] AFB STAIN
We always try to put them in the same slide, but sometimes it doesn’t work out the way. Either would be acceptable. Sent from my iPhone > On Oct 4, 2018, at 12:35 AM, Tony Henwood (SCHN) via Histonet > wrote: > > We do, > > It works well > > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Principal Scientist, the Children's Hospital at Westmead > Adjunct Fellow, School of Medicine, University of Western Sydney > Tel: 612 9845 3306 > Fax: 612 9845 3318 > Pathology Department > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > > -Original Message- > From: ADESUPO ADESUYI via Histonet [mailto:histonet@lists.utsouthwestern.edu] > Sent: Thursday, 4 October 2018 12:54 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] AFB STAIN > > Hello, > I have a question please. For the AFB Stain, do we put both the > control tissue and the patient on the same slide? > > Thanks, > Ade > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This message is intended for the addressee named and may contain confidential > information. If you are not the intended recipient, please delete it and > notify the sender. > > Views expressed in this message are those of the individual sender, and are > not necessarily the views of NSW Health or any of its entities. > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] AFB STAIN
We do, It works well Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: ADESUPO ADESUYI via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, 4 October 2018 12:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AFB STAIN Hello, I have a question please. For the AFB Stain, do we put both the control tissue and the patient on the same slide? Thanks, Ade ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] AFB STAIN
Hello, I have a question please. For the AFB Stain, do we put both the control tissue and the patient on the same slide? Thanks, Ade ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] AFB Stain on Cytospin Preps
Greg, I'm not sure if anyone has responded to your question, the waxy coat of acid-fast bacilli is sensitive to xylene and alcohol not to acid. Carbol Fuchsin is soluble in acid alcohol but if the waxy coat of the bacteria is intact, it will hold the stain. What I've suggested before is a cyto-spin preparation of unfixed fluid avoiding any alcohol or xylene even for dehydration and clearing. Hope this helps. J Good day colleagues, We are occasionally being asked to do an acid-fast stain (Ziehl-Neelsen) on a alcohol-fixed cytospin preparation from a cytology fluid. Typically in Cytology, if a specimen is very bloody, we will add acetic acid to lyse the red cells. Does anyone know if acetic acid would have any adverse affect on the stainability of AFB organisms if present? Thank you, Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] AFB Stain on Cytospin Preps
Good day colleagues, We are occasionally being asked to do an acid-fast stain (Ziehl-Neelsen) on a alcohol-fixed cytospin preparation from a cytology fluid. Typically in Cytology, if a specimen is very bloody, we will add acetic acid to lyse the red cells. Does anyone know if acetic acid would have any adverse affect on the stainability of AFB organisms if present? Thank you, Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] AFB Stain
Hi, I want to know whether it is compulsory to use two controls (positive & negative) for AFB Stain. Thanks, Banjo Adesuyi == CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] AFB stain
We run the AFB on the Dako Artisan - recently it has been coming off with VERY intense blue staining. Tech. support offered a new kit - same problem. We lessened the time and got the same result. All other stains on the Artisan are working. Thoughts are appreciated! Nancy Schmitt HT, MLT(ASCP) Histology Coordinator United Clinical Laboratories Dubuque, IA 52001 NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] AFB stain
Have any of you know heard of AFB will picking up in any other organisms beside mycobacterium? Amy Disclaimer: The information in this message is confidential. If you are not the intended recipient, do not disclose, copy, or distribute this message, and please immediately contact the sender. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
