[Histonet] ARC immunohistochemistry- "sticky sections" in DAB
Hi Gabrielle, Are you doing floating sections? Did you cut the tissue recently? Do you notice your tissue is also "sticky"? If you do, there is a possibility that the tissue may be infected by bacteria. Try washing the tissue with PBS or TBS with 0.1% sodium azide a couple time. Sometimes it help. Good Luck Best, Michelle ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ARC immunohistochemistry- "sticky sections" in DAB
To whom it may concern, I am a undergraduate researcher at Vassar College. I am performing immunohistochemistry on mice using Arc primary, anti-mouse biotinylated IgG (1:200),ABC (Vector labs) and DAB. So far we have gotten really great staining, however the last immuno I ran, the sections, once in DAB were sticking to the bottom of the wells. Does anyone have any idea as to why this would happen? Thank you! Best, Gabrielle ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
