Hello Paula,
This isn't an answer to your question, because I do use the Millipore
antibody (MAB805) and it works quite well on a Dako platform or done
manually. It requires enzyme digestion antigen retrieval. I use it at a
1:400 dilution (45 min) with a polymer detection system (45 min).
I'm not sure why it works on one platform but not another.
Best of luck,
Jan Shivers
Senior Scientist
IHC/Histology Section Manager
Pathology Teaching Program
Veterinary Diagnostic Laboratory
University of Minnesota
1333 Gortner Ave.
St. Paul, MN 55108
612-624-7297
shive...@umn.edu
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On Thu, Aug 3, 2017 at 8:47 PM, Tony Henwood (SCHN) via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> Hi Paula,
>
> We use Adenovirus antibody from BioSB (Diagnostic Technology)
> Cat Number BSB 5040
> Clone 20/11 and 2/6
> Isotype IgG1/K
> Dilution1:200 with citrate retrieval on the Bond 3
>
> Regards
> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
> Principal Scientist, the Children's Hospital at Westmead
> Adjunct Fellow, School of Medicine, University of Western Sydney
> Tel: 612 9845 3306
> Fax: 612 9845 3318
> Pathology Department
> the children's hospital at westmead
> Cnr Hawkesbury Road and Hainsworth Street, Westmead
> Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
>
> -Original Message-
> From: P Sicurello via Histonet [mailto:histonet@lists.utsouthwestern.edu]
> Sent: Friday, 4 August 2017 8:50 AM
> To: HistoNet
> Subject: [Histonet] Adenovirus antibody?
>
> Hello Listers,
>
> I am listing to starboard on this one. We are using the Ventana Ultra
> staining platform and have had a bit of trouble with our Adenovirus
> stains. We have used Abcam and Millipore to no avail.
>
> Do you have suggestions about another vendor to try? We are a clinical
> lab so we only stain human tissue. IVD is preferred but ASR and RUO are OK
> too.
>
> Any suggestions are gratefully accepted.
>
> Thank you in advance.
>
> Sincerely,
>
> Paula Sicurello, HTL (ASCP)CM
>
> Histotechnology Specialist
>
> UC San Diego Health
>
> 200 Arbor Drive
>
> San Diego, CA 92103
>
> (P): 619-543-2872 <#>
>
>
>
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