Re: [Histonet] Agarose embedded tissue arrays embedded in paraffin block
Exactly, I process the agar,not the tissue in it Cells,organoids, spheroids. Bernice -Original Message- From: Colleen Forster via Histonet Sent: Friday, May 17, 2024 3:07 PM To: Jay Lundgren Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Agarose embedded tissue arrays embedded in paraffin block Chistopher, Were these small pieces of tissue? If they are in an agar (such as histogel) they would need to have been processed overnight to ensure the agar is completely dehydrated during processing. The sample, no matter how small, is protected and processes perfectly. IF you ran a short run with the agar it will not have [processed properly. I was never able to salvage those samples. It took me a couple times to figure out the solution. Just a thought. Colleen Forster HT(ASCP)QIHC On Fri, May 17, 2024 at 2:32 PM Jay Lundgren via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Where did the agarose come from? > > On Fri, May 17, 2024 at 12:09 PM Otto, Christopher M via Histonet < > histonet@lists.utsouthwestern.edu> wrote: > > > > > > > Hello everyone! > > > > I'm having trouble sectioning tissue array blocks where the array > > is in agarose embedded into a paraffin block. I've chilled the > > blocks and I'm sectioning on a rotary microtome, at 5 microns, with > > a high profile Accuedge blade. The paraffin surrounding the agarose > > sections normally, > but > > the agarose portion of the block causes the blade to "skip" across > > it slightly and even chip out as if my blade isn't snug in the blade > > holder (it is). If I do get a tiny portion of agarose on my section > > to float > out > > on the waterbath it flies away (like adding ETOH to a waterbath with > > sections already on it.) Everyone I have asked about this says the > > agarose should section normally with the paraffin like any other > > FFPE blocks. Any ideas on why this agarose is behaving this way for > > me? Thank you in advance! > > > > > > > > ___ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/ > > listinfo/histonet__;!!Dq0X2DkFhyF93HkjWTBQKhk!SEM0o5vbbKuzHCJUAy39eR > > ALW6YwlWuJkF7rTMW9nJ_DRMWtgXqBRmD4yuNChwCHd4H_uCmrPk4dybLFdX60UH_YOK > > Z8ESaaEB3125Zq$ > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/li > stinfo/histonet__;!!Dq0X2DkFhyF93HkjWTBQKhk!SEM0o5vbbKuzHCJUAy39eRALW6 > YwlWuJkF7rTMW9nJ_DRMWtgXqBRmD4yuNChwCHd4H_uCmrPk4dybLFdX60UH_YOKZ8ESaa > EB3125Zq$ > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 321 Church St. SE Minneapolis, MN 55455 612-626-1930 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!Dq0X2DkFhyF93HkjWTBQKhk!SEM0o5vbbKuzHCJUAy39eRALW6YwlWuJkF7rTMW9nJ_DRMWtgXqBRmD4yuNChwCHd4H_uCmrPk4dybLFdX60UH_YOKZ8ESaaEB3125Zq$ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Agarose embedded tissue arrays embedded in paraffin block
Chistopher, Were these small pieces of tissue? If they are in an agar (such as histogel) they would need to have been processed overnight to ensure the agar is completely dehydrated during processing. The sample, no matter how small, is protected and processes perfectly. IF you ran a short run with the agar it will not have [processed properly. I was never able to salvage those samples. It took me a couple times to figure out the solution. Just a thought. Colleen Forster HT(ASCP)QIHC On Fri, May 17, 2024 at 2:32 PM Jay Lundgren via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Where did the agarose come from? > > On Fri, May 17, 2024 at 12:09 PM Otto, Christopher M via Histonet < > histonet@lists.utsouthwestern.edu> wrote: > > > > > > > Hello everyone! > > > > I'm having trouble sectioning tissue array blocks where the array is in > > agarose embedded into a paraffin block. I've chilled the blocks and I'm > > sectioning on a rotary microtome, at 5 microns, with a high profile > > Accuedge blade. The paraffin surrounding the agarose sections normally, > but > > the agarose portion of the block causes the blade to "skip" across it > > slightly and even chip out as if my blade isn't snug in the blade holder > > (it is). If I do get a tiny portion of agarose on my section to float > out > > on the waterbath it flies away (like adding ETOH to a waterbath with > > sections already on it.) Everyone I have asked about this says the > > agarose should section normally with the paraffin like any other FFPE > > blocks. Any ideas on why this agarose is behaving this way for me? Thank > > you in advance! > > > > > > > > ___ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 321 Church St. SE Minneapolis, MN 55455 612-626-1930 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Agarose embedded tissue arrays embedded in paraffin block
Where did the agarose come from? On Fri, May 17, 2024 at 12:09 PM Otto, Christopher M via Histonet < histonet@lists.utsouthwestern.edu> wrote: > > > Hello everyone! > > I'm having trouble sectioning tissue array blocks where the array is in > agarose embedded into a paraffin block. I've chilled the blocks and I'm > sectioning on a rotary microtome, at 5 microns, with a high profile > Accuedge blade. The paraffin surrounding the agarose sections normally, but > the agarose portion of the block causes the blade to "skip" across it > slightly and even chip out as if my blade isn't snug in the blade holder > (it is). If I do get a tiny portion of agarose on my section to float out > on the waterbath it flies away (like adding ETOH to a waterbath with > sections already on it.) Everyone I have asked about this says the > agarose should section normally with the paraffin like any other FFPE > blocks. Any ideas on why this agarose is behaving this way for me? Thank > you in advance! > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Agarose embedded tissue arrays embedded in paraffin block
Hello everyone! I'm having trouble sectioning tissue array blocks where the array is in agarose embedded into a paraffin block. I've chilled the blocks and I'm sectioning on a rotary microtome, at 5 microns, with a high profile Accuedge blade. The paraffin surrounding the agarose sections normally, but the agarose portion of the block causes the blade to "skip" across it slightly and even chip out as if my blade isn't snug in the blade holder (it is). If I do get a tiny portion of agarose on my section to float out on the waterbath it flies away (like adding ETOH to a waterbath with sections already on it.) Everyone I have asked about this says the agarose should section normally with the paraffin like any other FFPE blocks. Any ideas on why this agarose is behaving this way for me? Thank you in advance! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
