[Histonet] Air Bubbles
Has anyone encountered the following problem. Tissue cut and is processed well. There is no distortion at a cellular level. However anywhere from 30 to 2 days later there are air bubbles under the coverslip. I know for certain there aren't even the slightest bit of air bubbles when coverslipped. I have a feeling it could still be a processing issue as I ran a few tests like coverslipping blank slides and tissue sections from a year ago when there was no issues. If anyone has any suggestions as to what the problem could be and potential solutions please get back to me as soon as possible as this is becoming a major issue for my lab. -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Air Bubbles
There might be air in the mounting media that gives rise to bubbles when drying starts to occur. One method to try is to use an ultrasonic bath to remove the air from the bottle containing the mounting media before using. We used to do this with aqueous mounting media. Barry On Fri, Jun 10, 2016 at 9:15 AM, Karen Pfaff via Histonet < histonet@lists.utsouthwestern.edu> wrote: > > > > There might not be enough mounting media placed on slide. So when slide is > wet, there are no air bubble but after a couple of days the xylene or > xylene substitute will evaporate and leave you with patchy areas. Has there > been someone new coverslipping? I have been one of those people that put > less mounting media on because the stage of the microscope stays cleaner, > however, I discovered that I get air bubbles after it dries. Try putting a > line of mounting media down the entire slide not just 2 or 3 drops. Might > help.Karen PfaffLead HT Skin Cancer CenterMohs lab > > Sent from my U.S. Cellular® Smartphone > > Original message > From: Charles Riley via Histonet <histonet@lists.utsouthwestern.edu> > Date: 06/10/2016 9:00 AM (GMT-06:00) > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Air Bubbles > > Hello everyone, > > Recently we have been having an issue with a majority of our slides having > air bubbles under the coverslips. When they are originally coverslipped > there are no bubbles and any present are pushed out and the sides are all > wiped dry. We have not changed any reagent or processes in over two years. > Can anyone offer suggestions as to why this might be occurring or tips to > fix the issue? > > -- > > Charles Riley HT(ASCP)CM > > Histopathology Coordinator/ Mohs > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Air Bubbles
Hello everyone, Recently we have been having an issue with a majority of our slides having air bubbles under the coverslips. When they are originally coverslipped there are no bubbles and any present are pushed out and the sides are all wiped dry. We have not changed any reagent or processes in over two years. Can anyone offer suggestions as to why this might be occurring or tips to fix the issue? -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Air bubbles in Coverslipper
we often have to change the dispensing nozzle when this occurs. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Anna Huntley Coffey Sent: Friday, August 02, 2013 3:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Air bubbles in Coverslipper Hi Histonetters! We have a Leica CV5030 coverslipper in our lab and we regularly (~ once a week or so) have trouble with large air bubbles showing up in a significant number of our slides. We have flushed the hose from the mounting medium through the needle with xylenes which sometimes works to clear the bubbles and sometimes does not. Has anyone else had trouble with this and possibly found a solution? I would really appreciate your advice! Thanks, Anna ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
AW: [Histonet] Air bubbles in Coverslipper
Hi, We always have more than one medium-bottle prepared. At least three are filled in advance. So entrapped air can go away until usage and the oldest is the one for exchange. Gudrun -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Anna Huntley Coffey Gesendet: Freitag, 02. August 2013 21:56 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Air bubbles in Coverslipper Hi Histonetters! We have a Leica CV5030 coverslipper in our lab and we regularly (~ once a week or so) have trouble with large air bubbles showing up in a significant number of our slides. We have flushed the hose from the mounting medium through the needle with xylenes which sometimes works to clear the bubbles and sometimes does not. Has anyone else had trouble with this and possibly found a solution? I would really appreciate your advice! Thanks, Anna ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Air bubbles in Coverslipper
We have had success with this also. If you got the unit new, I believe that there should have been one extra bottle with it. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Saturday, August 03, 2013 5:16 AM To: 'Anna Huntley Coffey' Cc: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] Air bubbles in Coverslipper Hi, We always have more than one medium-bottle prepared. At least three are filled in advance. So entrapped air can go away until usage and the oldest is the one for exchange. Gudrun -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Anna Huntley Coffey Gesendet: Freitag, 02. August 2013 21:56 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Air bubbles in Coverslipper Hi Histonetters! We have a Leica CV5030 coverslipper in our lab and we regularly (~ once a week or so) have trouble with large air bubbles showing up in a significant number of our slides. We have flushed the hose from the mounting medium through the needle with xylenes which sometimes works to clear the bubbles and sometimes does not. Has anyone else had trouble with this and possibly found a solution? I would really appreciate your advice! Thanks, Anna ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Air bubbles in Coverslipper
I do not use an automatic cover-slipper so don't know if this will help. However the delete button is nearby. When we wanted to remove trapped air from any solution we gave the container a short period in an ultrasonic bath - 10 seconds or so (depending on viscosity of the solution. This worked well for some of the media we used before embedding in electron microscopy. Barry From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni [trathbo...@somerset-healthcare.com] Sent: Saturday, August 03, 2013 7:24 AM To: 'gu.l...@gmx.at'; 'Anna Huntley Coffey' Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Air bubbles in Coverslipper We have had success with this also. If you got the unit new, I believe that there should have been one extra bottle with it. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Saturday, August 03, 2013 5:16 AM To: 'Anna Huntley Coffey' Cc: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] Air bubbles in Coverslipper Hi, We always have more than one medium-bottle prepared. At least three are filled in advance. So entrapped air can go away until usage and the oldest is the one for exchange. Gudrun -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Anna Huntley Coffey Gesendet: Freitag, 02. August 2013 21:56 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Air bubbles in Coverslipper Hi Histonetters! We have a Leica CV5030 coverslipper in our lab and we regularly (~ once a week or so) have trouble with large air bubbles showing up in a significant number of our slides. We have flushed the hose from the mounting medium through the needle with xylenes which sometimes works to clear the bubbles and sometimes does not. Has anyone else had trouble with this and possibly found a solution? I would really appreciate your advice! Thanks, Anna ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Air bubbles in Coverslipper
Hi Histonetters! We have a Leica CV5030 coverslipper in our lab and we regularly (~ once a week or so) have trouble with large air bubbles showing up in a significant number of our slides. We have flushed the hose from the mounting medium through the needle with xylenes which sometimes works to clear the bubbles and sometimes does not. Has anyone else had trouble with this and possibly found a solution? I would really appreciate your advice! Thanks, Anna ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
