Re: [Histonet] Amyloid staining on frozen muscle bx's
The method at the address below should work on cryostat sections. http://stainsfile.info/StainsFile/stain/amyloid/siriusllew.htm Bryan Llewellyn - Original Message - From: Tony Henwood antho...@chw.edu.au To: Sharon Allen sal...@exchange.hsc.mb.ca; histo...@pathology.swmed.edu Sent: Wednesday, August 19, 2009 3:26 PM Subject: RE: [Histonet] Amyloid staining on frozen muscle bx's Sharon, For successful amyloid staining with Congo red, formalin fixation might be required. I have not been able to find any references for this except that formalin is used in the demonstration of amyloid in fat aspirations (reference: Duston et al (1987) Am J Med 82:412-414) Have you tried one of the metachromatic dyes (eg Crystal Violet)? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon Allen Sent: Thursday, 20 August 2009 6:57 AM To: histo...@pathology.swmed.edu Subject: [Histonet] Amyloid staining on frozen muscle bx's Hi, What stain works for amyloid on frozen muscle bx tissue? I did the a Congo Red stain without much success. Thanks Sharon sal...@hsc.mb.ca * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Amyloid staining on frozen muscle bx's
Hi, What stain works for amyloid on frozen muscle bx tissue? I did the a Congo Red stain without much success. Thanks Sharon sal...@hsc.mb.ca This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destiné à la personne ou aux personnes à qui il est adressé. Il peut contenir des informations privilégiées ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autorisée est strictement défendue. Si vous n'êtes pas le destinataire de ce message, veuillez en informer l'expéditeur immédiatement et lui remettre l'original. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Amyloid staining on frozen muscle bx's
Sharon, For successful amyloid staining with Congo red, formalin fixation might be required. I have not been able to find any references for this except that formalin is used in the demonstration of amyloid in fat aspirations (reference: Duston et al (1987) Am J Med 82:412-414) Have you tried one of the metachromatic dyes (eg Crystal Violet)? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon Allen Sent: Thursday, 20 August 2009 6:57 AM To: histo...@pathology.swmed.edu Subject: [Histonet] Amyloid staining on frozen muscle bx's Hi, What stain works for amyloid on frozen muscle bx tissue? I did the a Congo Red stain without much success. Thanks Sharon sal...@hsc.mb.ca * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
