Re: [Histonet] Artifacts in histology section
It's very difficult to diagnosis a problem such as this without hands on. But one thing that I noticed years ago that was so simple could cause this. A build up of tissues on the back of the blade holder. Worth a look. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 Joseph Saby saby_josep...@yahoo.com Sent by: histonet-boun...@lists.utsouthwestern.edu 07/25/2010 12:49 PM To Aazath Raj aaz...@hotmail.com, histonet@lists.utsouthwestern.edu cc Subject Re: [Histonet] Artifacts in histology section What you are describing might be microchatter. These will be sharp parallel lines/cracks that run parallel to the knife edge and are only visible under the microscope. The usuall cause is a combination of overprocessing and rough facing that is too aggressive and/or with too dull a blade. Overprocessing makes the tissue very hard and somewhat brittle. The thick sections/dull knife cause the tissue to compress and then release, causing the chatter. The actual danage is in the block face. Once you have the problem in a block, if the tissue is thick enough, you might be able to repeatedly soak the block in ice water and gently (with a fairly sharp knife) reface. With luck, you might be able to get through the damaged block face. Another artifact I have seen is similar, but the chatter appears very blurry. This is usually caused be poor fixation/processing, then oversoaking the blocks after facing. The trick here is to reface the block, then chill it without exposure to water. I've sectioned such blocks after placing them in a freezer to chill them thoroughly. This will help to obtain a section, but may not fix the staining problems that might show up later. Good luck! Joe Saby, BA HT From: Aazath Raj aaz...@hotmail.com To: histonet@lists.utsouthwestern.edu Sent: Fri, July 23, 2010 11:26:28 AM Subject: [Histonet] Artifacts in histology section Dear Friends, I am an Histology Technologist. I am having a problem here,while sectioning am not seeing and scoring artifacts on the section but in the microscope am seeing a tearing artifacts particularly in endoscopy biopsies. am not able to locate where is the problem,is that because of blades or due to micro-crystallization of wax or due to any processing problem. Its not consistently in all but i get it on some blocks every day. Can any one help me in sorting it out. If anybody is interested in will send the picture of those section. with regards, Aazathraj.P Technical Officer, Apollo Hospitals-chennai India. aaz...@hotmail.com _ The latest in fashion and style in MSN Lifestyle http://lifestyle.in.msn.com/___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Artifacts in histology section
What you are describing might be microchatter. These will be sharp parallel lines/cracks that run parallel to the knife edge and are only visible under the microscope. The usuall cause is a combination of overprocessing and rough facing that is too aggressive and/or with too dull a blade. Overprocessing makes the tissue very hard and somewhat brittle. The thick sections/dull knife cause the tissue to compress and then release, causing the chatter. The actual danage is in the block face. Once you have the problem in a block, if the tissue is thick enough, you might be able to repeatedly soak the block in ice water and gently (with a fairly sharp knife) reface. With luck, you might be able to get through the damaged block face. Another artifact I have seen is similar, but the chatter appears very blurry. This is usually caused be poor fixation/processing, then oversoaking the blocks after facing. The trick here is to reface the block, then chill it without exposure to water. I've sectioned such blocks after placing them in a freezer to chill them thoroughly. This will help to obtain a section, but may not fix the staining problems that might show up later. Good luck! Joe Saby, BA HT From: Aazath Raj aaz...@hotmail.com To: histonet@lists.utsouthwestern.edu Sent: Fri, July 23, 2010 11:26:28 AM Subject: [Histonet] Artifacts in histology section Dear Friends, I am an Histology Technologist. I am having a problem here,while sectioning am not seeing and scoring artifacts on the section but in the microscope am seeing a tearing artifacts particularly in endoscopy biopsies. am not able to locate where is the problem,is that because of blades or due to micro-crystallization of wax or due to any processing problem. Its not consistently in all but i get it on some blocks every day. Can any one help me in sorting it out. If anybody is interested in will send the picture of those section. with regards, Aazathraj.P Technical Officer, Apollo Hospitals-chennai India. aaz...@hotmail.com _ The latest in fashion and style in MSN Lifestyle http://lifestyle.in.msn.com/___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Artifacts in histology section
Dear Friends, I am an Histology Technologist. I am having a problem here,while sectioning am not seeing and scoring artifacts on the section but in the microscope am seeing a tearing artifacts particularly in endoscopy biopsies. am not able to locate where is the problem,is that because of blades or due to micro-crystallization of wax or due to any processing problem. Its not consistently in all but i get it on some blocks every day. Can any one help me in sorting it out. If anybody is interested in will send the picture of those section. with regards, Aazathraj.P Technical Officer, Apollo Hospitals-chennai India. aaz...@hotmail.com _ The latest in fashion and style in MSN Lifestyle http://lifestyle.in.msn.com/___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Artifacts in histology section
Of the 3 probable causes, the most likely to be is the blade edge not uniformly sharp René J. --- On Fri, 7/23/10, Aazath Raj aaz...@hotmail.com wrote: From: Aazath Raj aaz...@hotmail.com Subject: [Histonet] Artifacts in histology section To: histonet@lists.utsouthwestern.edu Date: Friday, July 23, 2010, 11:26 AM Dear Friends, I am an Histology Technologist. I am having a problem here,while sectioning am not seeing and scoring artifacts on the section but in the microscope am seeing a tearing artifacts particularly in endoscopy biopsies. am not able to locate where is the problem,is that because of blades or due to micro-crystallization of wax or due to any processing problem. Its not consistently in all but i get it on some blocks every day. Can any one help me in sorting it out. If anybody is interested in will send the picture of those section. with regards, Aazathraj.P Technical Officer, Apollo Hospitals-chennai India. aaz...@hotmail.com _ The latest in fashion and style in MSN Lifestyle http://lifestyle.in.msn.com/___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
