Karie,
If you are looking for microdamage or are using basic fuchsin, I have
been taught to bulk stain with 1% basic fuchsin in your processing
solutions (i.e. 1% BF in 70% EtOH, 1% BF in 80% EtOH, 1% BF in 95%
EtOH, and 1% BF in 100% EtOH) and then finish as you would with the
remaining steps for your MMA protocol (i.e. xylenes, MMA +DBP, etc.)
to achieve a fully polymerized bulk stained specimen. You then cut,
grind, and polish as required for use with the EXAKT system. I was
told the reason you process with the BF is because the cutting and
grinding causes additional microdamage and if you stain first the
additional damage will not be stained.
If you are talking about labeling bone for calculations of BFR or MAR
and the animal did not receive timed injections of calcein, alizarin,
tetracycline, or some other fluorescent bone label before necropsy,
then you could try doing a 0.6% FA etch for 30 seconds followed with
Sanderson's Rapid Bone Stain for 3 minutes, brief tap water rinse, and
blot dry.
What is the exact specimen you are working with and what do you
specifically wish to demonstrate
Jack
On Dec 1, 2009, at 10:04 AM, Karie Reaser krea...@vet.upenn.edu wrote:
Is it ok to process cortical bone, embed in MMA, section and grind
on the Exakt system. Last do the bone labelling stain? Or do I have
to do the bone labelling stain prior to embedding, sectioning and
mounting on slides? Any suggestions would be greatly appreciated.
--
Karie L Reaser A.S.
New Bolton Center
University of Pennsylvania
School of Veterinary Medicine
Comparative Orthopedic Research Laboratory
382 W Street Road
Kennett Square, PA. 19348
Phone:610-925-6278
Fax:610-925-8120
Email:krea...@vet.upenn.edu
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