Martha,
We reported our study of 15 brands of adhesive slides for "wash off" and
found little difference among the different slides when well-fixed cell
culture material was examined.
On the other hand, poorly-fixed breast cancer tissues did appear to adhere
more strongly to some slides than others (TOMO being among the best).
Additional factors need to be considered, though, and I note that your
baking procedure is different from what is recommended by many slide
vendors.
In general, baking at 60-65 deg C for 1 hour is said to be optimal,
although we did not examine that parameter specifically.
Please see our poster at https://www.arrayscience.com/publications#Posters
Best regards,
Regan
Regan Fulton, M.D., Ph.D.
CEO and Co-Founder
Array Science, LLC
475 Gate 5 Road, #100
Sausalito, CA 94965
(415) 577-7360
email: ful...@arrayscience.com
www.arrayscience.com
On Thu, Sep 23, 2021 at 1:02 PM Martha Ward-Pathology via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> It was brought to my attention that we had significant washing on 3 of 8
> bone marrow clot sections the other day; this is not the first time so we
> would like to get to the bottom of this. We use positively charged slides
> and all 8 cases were cut and run the same morning but allowed to air dry
> and then bake at 60C for 20 minutes before being run on our Bond 3
> stainer. Has anyone out there experienced this type of problem and if so,
> what were your solutions?The repeat of the 3 cases today showed similar
> washing of tissue.
>
> This hasn't just started but has occurred periodically but the
> pathologists have tried to live with it and usually we can finally get
> enough tissue to stay on after 1-2 attempts. Suggestions include cutting
> and drying the slides overnight and/or going to a gelatinated slide versus
> a sialylated slide. We have been using this particular brand of positive
> charged slide with good results for several years and rarely have issues
> with other tissue types unless they are particularly bloody.
>
> Thoughts or suggestions are greatly appreciated.
>
>
> Martha Ward MT(ASCP) QIHC
> Atrium Health Wake Forest Baptist
>
>
>
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