AW: [Histonet] C4D Antibody
We work with the Benchmark XT, ultraview kit (polymer), CC1 retrieval (pH 8-9) 30 min, C4d from Zytomed 1:25 32min, with amplification. Gudrun Lang Akh Linz, Austria -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von kk...@bidmc.harvard.edu Gesendet: Freitag, 21. August 2009 19:39 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] C4D Antibody Just wondering if anyone out there routinely works with C4d. I was wondering what you use for retrieval and the Type of detection kit you use. Thanks. Kwadwo Kwaa. BIDMC Pathology Department IHC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
AW: [Histonet] C4d Antibody
Hi César we use mo-a-C4d, clone CL314 (Quidel A213) at 1.25 ug/ml (1:800) for cryosections of kidney biopsies (indirect immunofluorescence), it won't work on FFPE material (at least in our hands). For FFPE, Biomedica's rb-a-C4d (BI-RC4D) works fine at ca. 6 ug/ml (1:30) after HIER in a high pH buffer (Tris-EDTA, pH 9.0; Tris-Urea, pH 9.5). Hope this helps. Best regards Andi Kappeler Institute of Pathology, University of Bern, Switzerland -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Cesar Francisco Romero Gesendet: Dienstag, 24. März 2009 05:01 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] C4d Antibody Dear People from Histonet Could you share your experience with C4d Antibody ? I couldn't get any results with the mouse monoclonal from Quidel. I will try now the rabbit polyclonal ( C4dpAb )from Biomedica - Grouppe. I work in a Public Hospital with many kidney transplantations per year. Thank You in advance. César Romero Buenos Aires Argentina _ ¿Querés saber cómo va a estar el clima mañana? Ingresá ahora a MSN http://tiempo.ar.msn.com/___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] C4d Antibody
We get our from Alpco. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! Cesar Francisco Romero chesar...@hotmail.com 3/24/2009 12:00 AM Dear People from Histonet Could you share your experience with C4d Antibody ? I couldn't get any results with the mouse monoclonal from Quidel. I will try now the rabbit polyclonal ( C4dpAb )from Biomedica - Grouppe. I work in a Public Hospital with many kidney transplantations per year. Thank You in advance. César Romero Buenos Aires Argentina _ ¿Querés saber cómo va a estar el clima mañana? Ingresá ahora a MSN http://tiempo.ar.msn.com/___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
AW: [Histonet] C4d Antibody
Our's is from Zytomed, a polyclonal rabbit (a12-5000). We work with the benchmark XT, 1:25 with amplifier. Gudrun -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Cesar Francisco Romero Gesendet: Dienstag, 24. März 2009 05:01 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] C4d Antibody Dear People from Histonet Could you share your experience with C4d Antibody ? I couldn't get any results with the mouse monoclonal from Quidel. I will try now the rabbit polyclonal ( C4dpAb )from Biomedica - Grouppe. I work in a Public Hospital with many kidney transplantations per year. Thank You in advance. César Romero Buenos Aires Argentina _ ¿Querés saber cómo va a estar el clima mañana? Ingresá ahora a MSN http://tiempo.ar.msn.com/___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] C4d Antibody
Dear People from Histonet Could you share your experience with C4d Antibody ? I couldn't get any results with the mouse monoclonal from Quidel. I will try now the rabbit polyclonal ( C4dpAb )from Biomedica - Grouppe. I work in a Public Hospital with many kidney transplantations per year. Thank You in advance. César Romero Buenos Aires Argentina _ ¿Querés saber cómo va a estar el clima mañana? Ingresá ahora a MSN http://tiempo.ar.msn.com/___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
