Re: [Histonet] Chicago Sky Blue stain vs. KOH - striking comparison
The names Chicago blue and sky blue have been applied to various disazo dyes used in the past for direct dyeing of cotton. The name Chicago sky blue does not appear as a principal name or a synonym in either the 9th (1977) or the 10th (2002) editions of Conn's Biological Stains. In Conn9, there is a reference for staining fungi (Kuranz 1964) with ink blue, which was believed to be Chicago blue 6B (CI 24410, Direct blue 1). This dye has also been used to quench autofluorescence prior to immunofluorescent staining, often as pontamine sky blue 6B or Niagara blue 6B. Closely related dyes are Chicago blue 4B (CI 24395, also called benzo sky blue 2B and brilliant benzo blue 6B) and benzo sky blue (CI 24400, Direct blue 15, also called sky blue and pure blue). Probably Chicago blue 6B (CI 24410, Direct blue 1) is the only one of these likely to be available. In the 1970s it was still an article of commerce with more than 70 brand names, but by 2002 it was no longer used industrially. The CI number and application name are the best identifiers, but remember that dyes often are mixtures and sometimes are mislabelled. None of these blue disazo dyes are certified by the Biological Stain Commission. John Kiernan Anatomy Cell Biology UWO, London, Canada. = = = On 10/11/11, Harrison, Sandra C. sandra.harris...@va.gov wrote: Does anyone have ordering information for a stain called Chicago Sky Blue(CSB)? I just saw an article in the NOV issue of ASCP LABMEDICINE (page 649) comparing KOH vs. CSB and, wow, is that a beautiful stain. The results were dramatic, with about a 25-50% increase in the Dermatophytes identified and about a 5X increase in the Pityriasis versicolor identification. Thanks, Sandy C. Harrison, HTL (ASCP) Histology Supervisor ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Chicago Sky Blue stain vs. KOH - striking comparison
Does anyone have ordering information for a stain called Chicago Sky Blue(CSB)? I just saw an article in the NOV issue of ASCP LABMEDICINE (page 649) comparing KOH vs. CSB and, wow, is that a beautiful stain. The results were dramatic, with about a 25-50% increase in the Dermatophytes identified and about a 5X increase in the Pityriasis versicolor identification. Thanks, Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
FW: [Histonet] Chicago Sky Blue stain vs. KOH - striking comparison
We use Chicago Sky Blue 6B from Sigma C-8679 (2610-05-1). We make our solution as followed: 1g CSB 6B 1ml DiMethylsulphoxid 99ml Dist water Daniela Bodemer Research Assistant Surgical Research, Infection and Immunity Murdoch Childrens Research Institute The Royal Children's Hospital Flemington Road Parkville Victoria 3052 Australia T 03 9345 5930 T (03 9345 4116) E daniela.bode...@mcri.edu.au www.mcri.edu.au -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Harrison, Sandra C. Sent: Friday, 11 November 2011 4:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Chicago Sky Blue stain vs. KOH - striking comparison Does anyone have ordering information for a stain called Chicago Sky Blue(CSB)? I just saw an article in the NOV issue of ASCP LABMEDICINE (page 649) comparing KOH vs. CSB and, wow, is that a beautiful stain. The results were dramatic, with about a 25-50% increase in the Dermatophytes identified and about a 5X increase in the Pityriasis versicolor identification. Thanks, Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email If you have any question, please contact MCRI IT Helpdesk for further assistance. __ __ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
