[Histonet] Color in Sections with Adipose Tissue?
I've observed images on a computer screen with a person next to me insisting that the adipose tissue in HE stained tissue has a color versus areas with no tissue (open areas). Am I missing something, or do others see either a slight discoloration in adipose tissue, or a slightly different color, versus areas without tissue? I suspect it depends on section thickness, but given a 5 - 8 micron section it seems that whatever tissue might have been present would be scraped away with the blade. Jerry Sedgewick ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Color in Sections with Adipose Tissue?
Oh yeah, didn't think about frozen vs paraffin section since I'm an imaging guy. If it were frozen, would some remnant of the adipose tissue remain after sectioning? Thanks! On 9/4/2014 1:02 PM, jsjurc...@comcast.net wrote: Are you talking frozens here? *From: *jerry sedgewick jerrysedgew...@gmail.com *To: *Histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu *Sent: *Thursday, September 4, 2014 12:52:30 PM *Subject: *[Histonet] Color in Sections with Adipose Tissue? I've observed images on a computer screen with a person next to me insisting that the adipose tissue in HE stained tissue has a color versus areas with no tissue (open areas). Am I missing something, or do others see either a slight discoloration in adipose tissue, or a slightly different color, versus areas without tissue? I suspect it depends on section thickness, but given a 5 - 8 micron section it seems that whatever tissue might have been present would be scraped away with the blade. Jerry Sedgewick ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Color in Sections with Adipose Tissue?
Thanks, Colleen...Got it! On 9/4/2014 1:55 PM, Colleen Forster wrote: When I cut adipose tissues Jerry, that have been fixed and processed you will get a slide that appears clear or like tissue is missing in between the vessels. If you look under the scope you will see fine cell membranes that outline the fat cell. The fat dissolves during processing leaving only the membrane and vasculature. If they want to see the actual fat it has to be done by frozen section. Does this make sense? C On Thu, Sep 4, 2014 at 12:52 PM, jerry sedgewick jerrysedgew...@gmail.com mailto:jerrysedgew...@gmail.com wrote: I've observed images on a computer screen with a person next to me insisting that the adipose tissue in HE stained tissue has a color versus areas with no tissue (open areas). Am I missing something, or do others see either a slight discoloration in adipose tissue, or a slightly different color, versus areas without tissue? I suspect it depends on section thickness, but given a 5 - 8 micron section it seems that whatever tissue might have been present would be scraped away with the blade. Jerry Sedgewick ___ Histonet mailing list Histonet@lists.utsouthwestern.edu mailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
