No, once you have removed the coverslip, just wash the section thoroughly in
xylene to remove any thick remnant of the old permount, and reapply permount
and cover.
René J.
--- On Fri, 10/22/10, Lewis, Patrick patrick.le...@seattlechildrens.org wrote:
From: Lewis, Patrick patrick.le...@seattlechildrens.org
Subject: [Histonet] Coverslip removal question
To: Histonet@lists.utsouthwestern.edu
Date: Friday, October 22, 2010, 3:55 PM
Hi Histonetters
When I soak my slides in xylene to remove the coverslip, do I then need
to rehydrate them by washing them in 100% etoh,95% etoh,70% etoh, and
then H20.
And then drying and re applying permount?
Or can I go straight from xylene to drying to re-coversliping with
permount?
Patrick Lewis
Research Associate II-Bench| Infections and Prematurity
Seattle Children's Research Institute
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