[Histonet] Cryostat sectioning of frozen leaves
I am trying to cut frozen leaf sections at 6 and 8 microns. All I am getting is bunched up leaf. What is the trick/ I am cutting at -20 should be cutting at a lower temp and cutting thicker? Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1160 Pine Ave. W - Rm 312 Montreal, QC, Canada H3G 1Y6 Tel: 514-398-8270 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cryostat sectioning of FF lymph node
Good morning! My pathologists would like us to cut formalin fixed (not yet processed) tonsil tissue on a cryostat for DIF staining. Has anyone done this? I did a quick search that seemed to indicate that it was possible but that the architecture of the cells would be altered because of ice crystals and that it would be difficult to get the sections to stick. If you have any advice I would greatly appreciate it! Thanks, Erin Erin Martin, Histology Supervisor UCSF Dermatopathology and Oral Pathology Service 1701 Divisadero St, San Francisco, CA 94044 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error please contact the sender and delete the material from any computer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cryostat sectioning
Hi, We are searching for a fee for service lab experienced in cryostat sectioning of animal and human tissues. Any recommendation is appreciated. Thanks, Shu-Cheng Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] cryostat sectioning hairy skin
Hi, I have used a Leica cryostat for several years and found that for most types of tissue, it cuts best at an angle set at 0. I am interested in knowing how long it takes for the tissue to warm up from -80 to -20. Danny Brawn Prince Edward Island Canada - Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
