Re: [Histonet] Crystals forming in Hematoxylin

[Paula Keene Pierce via Histonet]

That is the aluminum salt precipitating out.
Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com

A sharp knife is nothing without a sharp eye. - Klingon Proverb 

On Thursday, July 9, 2020, 11:47:53 AM CDT, Terri Braud via Histonet 
 wrote:  
 
 Hello Histo Peeps - What are the clear crystals that form in Hematoxylin?  We 
use Richard-Allen 7211.  We love the stain, but occasionally, when a bottle has 
been previously opened, we find clear crystalline precipitate forming in the 
bottom.  Today, we had a huge one (over an 2" in greatest diameter) that was 
quite beautiful.  It had a few occlusions of purple streaks, but otherwise was 
clear.  Doesn't seem to affect the stain, so I was just curious.
Inquisitively yours, Terri

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal

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Re: [Histonet] Crystals forming in Hematoxylin

[Hannen, Valerie via Histonet]

Hi Terri,

The crystals form during cold winter months, usually during shipping or can 
form if the Hematoxylin 7211 is stored in a cold environment.  They also form 
due to the Mordant being put into solution at it's saturation point.
The crystals have no adverse effect on the stain itself.  If your bottle has a 
few crystals, just filter it and it should remain crystal free.  If there are a 
lot, you can gently warm it, using a "hot" plate and constant stirring until 
the crystals go back into solution, then filter it and is should be good to go. 
  I only learned this when I "googled" about it myself.  I hope this helps!!

Regards,

Valerie


Valerie Hannen,MLT(ASCP),HTL,SU (FL)
Section Chief, Histology
Parrish Medical Center
951 N. Washington Ave.
Titusville,Florida 32796
T: (321)268-6333 ext. 7506
F: (321) 268-6149
valerie.han...@parrishmed.com
www.parrishmed.com



-Original Message-
From: Terri Braud via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, July 09, 2020 12:39 PM
To: histonet@lists.utsouthwestern.edu
Subject: [EXTERNAL Sender] [Histonet] Crystals forming in Hematoxylin


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Begin Original Message:

--
Hello Histo Peeps - What are the clear crystals that form in Hematoxylin?  We 
use Richard-Allen 7211.  We love the stain, but occasionally, when a bottle has 
been previously opened, we find clear crystalline precipitate forming in the 
bottom.  Today, we had a huge one (over an 2" in greatest diameter) that was 
quite beautiful.  It had a few occlusions of purple streaks, but otherwise was 
clear.  Doesn't seem to affect the stain, so I was just curious.
Inquisitively yours, Terri

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal

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[Histonet] Crystals forming in Hematoxylin

[Terri Braud via Histonet]

Hello Histo Peeps - What are the clear crystals that form in Hematoxylin?  We 
use Richard-Allen 7211.  We love the stain, but occasionally, when a bottle has 
been previously opened, we find clear crystalline precipitate forming in the 
bottom.  Today, we had a huge one (over an 2" in greatest diameter) that was 
quite beautiful.  It had a few occlusions of purple streaks, but otherwise was 
clear.  Doesn't seem to affect the stain, so I was just curious.
Inquisitively yours, Terri

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal

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