[Histonet] Cyclin D1
Is anyone running Cyclin D1 on the Leica Bond? I have their ready to use antibody, but it hasn't been worked up on the Bond and my first try has left me with more background than I would like. Does anyone have it worked up with a protocol they would like to share? Joanne Clark, HT(ASCP)CM Director of Histology Pathology Consultants of New Mexico Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cyclin D1 IHC
Hello Histonetters, Has anyone done IHC using Cyclin D1 antibody? What vendor was used? What control tissue was used? Thank you. Donna Suresch - Merck Co. Donna L. Suresch Imaging Research Scientist Merck Research Laboratories Department of Imaging - West Point Campus Mail Stop: WP44KOffice: WP44-H129 770 Sumneytown Pike PO Box 4 West Point, PA 19486-0004 Phone: 215-652-7349 Fax: 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cyclin D1 IHC
We have used Neomarkers/Labvision cat.no. RM-9104 on mouse salivary glands and mammary glands. On Tue, Jul 24, 2012 at 11:16 AM, Suresch, Donna L. donna_sure...@merck.com wrote: Hello Histonetters, Has anyone done IHC using Cyclin D1 antibody? What vendor was used? What control tissue was used? Thank you. Donna Suresch - Merck Co. Donna L. Suresch Imaging Research Scientist Merck Research Laboratories Department of Imaging - West Point Campus Mail Stop: WP44KOffice: WP44-H129 770 Sumneytown Pike PO Box 4 West Point, PA 19486-0004 Phone: 215-652-7349 Fax: 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cyclin D1 IHC
Hi Donna, We use Dako #M3635 at 1:100. Mantle cell lymphoma is our control. Mark On Tue, Jul 24, 2012 at 8:16 AM, Suresch, Donna L. donna_sure...@merck.comwrote: Hello Histonetters, Has anyone done IHC using Cyclin D1 antibody? What vendor was used? What control tissue was used? Thank you. Donna Suresch - Merck Co. Donna L. Suresch Imaging Research Scientist Merck Research Laboratories Department of Imaging - West Point Campus Mail Stop: WP44KOffice: WP44-H129 770 Sumneytown Pike PO Box 4 West Point, PA 19486-0004 Phone: 215-652-7349 Fax: 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
