Re: [Histonet] Cytology/Histology Staining Question

[Tony Henwood (SCHN) via Histonet]

Yep,
We do.
We have a separate dehydration sequence for PAP and other special stains 
(separate from the eosin dehydration sequence).

We have a Leica (used to be Vision Biosystems) Autostainer

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Principal Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-Original Message-
From: Mullen, Mary via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Wednesday, 11 May 2016 12:55 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cytology/Histology Staining Question

Hello all,



I work in a small, low volume community hospital and was recently asked by a 
coworker why we do not just run both our cytology and histology slides on the 
same automated stainer (with their respective protocols).



What I am wanting to know is if there is anyone currently running both staining 
protocols on a single automated stainer using common alcohols/xylenes/water? 
What are the pros/cons? Has there been any cross-contamination issues?



We only run non-gyn cytology, all gyn cytology is sent out.







Thanks,



Mary K. Mullen, HTL(ASCP)CM
Histotechnologist
UPMC Northwest
Seneca, PA

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Re: [Histonet] Cytology/Histology Staining Question

[Hannen, Valerie via Histonet]

Mary,

We run both protocols on the same stainer, however, each protocol has it own 
set of reagents except for the common water wells and the two xylenes at the 
end before the final xylene.  We have the Leica ST 5020, which has 36 wells not 
including the 2 oven wells.

Hope this helps.


Valerie Hannen,MLT(ASCP),HTL,SU (FL)
Section Chief, Histology
Parrish Medical Center
951 N. Washington Ave.
Titusville,Florida 32796
T: (321)268-6333 ext. 7506
F: (321) 268-6149
valerie.han...@parrishmed.com
www.parrishmed.com


-Original Message-
From: Mullen, Mary via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, May 10, 2016 10:55 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cytology/Histology Staining Question

Hello all,



I work in a small, low volume community hospital and was recently asked by a 
coworker why we do not just run both our cytology and histology slides on the 
same automated stainer (with their respective protocols).



What I am wanting to know is if there is anyone currently running both staining 
protocols on a single automated stainer using common alcohols/xylenes/water? 
What are the pros/cons? Has there been any cross-contamination issues?



We only run non-gyn cytology, all gyn cytology is sent out.







Thanks,



Mary K. Mullen, HTL(ASCP)CM
Histotechnologist
UPMC Northwest
Seneca, PA

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Re: [Histonet] Cytology/Histology Staining Question (Mullen, Mary)

[O'Donnell, Lynn M. via Histonet]

This is the CAP regulation that talks about cross contamination.


CYP.04150 Cross-Contamination Phase I
There is a written procedure to prevent cross-contamination of specimens during
processing and staining.
NOTE: Procedures must prevent cross-contamination between gynecologic and 
non-gynecologic
specimens.
Also, procedures must prevent contamination among non-gynecologic cases when 
highly
cellular specimens are processed. Methods to minimize this potential problem 
may include
cytocentrifuge, filter, and monolayer preparations. Direct smears made from the 
sediment of
highly cellular cases should be stained after the other cases, and the staining 
fluids must be
changed or filtered between each of the highly cellular cases. One procedure to 
detect highly
cellular specimens is to use a toluidine blue, or other rapid stain, on a wet 
preparation. One
procedure to detect possible contamination is to insert a clean blank slide in 
each staining run
and examine it for contamination.
REFERENCES
1) Department of Health and Human Services, Centers for Medicare and Medicaid 
Services. Clinical laboratory improvement
amendments of 1988; final rule. Fed Register. 2003(Jan 24):7169 
[42CFR493.1274(b)(2-3)]




Lynn M. O'Donnell, CT (ASCP), MHA l Technical Specialist, Cytology
Danbury Hospital l lynn.o'donn...@wchn.org
tel: 203-739-6704  Fax: 203-739-6034




-Original Message-
From: T H via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, May 10, 2016 13:46
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Cytology/Histology Staining Question (Mullen, Mary)

Hey Mary,

The problem is not the machine, it is the reagents sharing that is the issue.  
You can use different reagents and protocols for the Histo and Cyto slides and 
on the same instrument.  You might even get away with changing your Alcohols 
and filtering everything else and see how that works.  Try it and run some 
blank slides through the stainer and see if anything is there from the cytology 
specimens.

I would personally have two separate sets of staining reagents to be on the 
safe side.

Good luck!!

Tim


Message: 7
Date: Tue, 10 May 2016 14:54:55 +
From: "Mullen, Mary" <mulle...@mail.magee.edu>
To: "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Subject: [Histonet] Cytology/Histology Staining Question
Message-ID:
<374dc72e6b29d44086f8ff3289351b2508823...@msxmbxnsprd39.acct.upmchs.net>

Content-Type: text/plain; charset="iso-8859-1"

Hello all,



I work in a small, low volume community hospital and was recently asked by a 
coworker why we do not just run both our cytology and histology slides on the 
same automated stainer (with their respective protocols).



What I am wanting to know is if there is anyone currently running both staining 
protocols on a single automated stainer using common alcohols/xylenes/water? 
What are the pros/cons? Has there been any cross-contamination issues?



We only run non-gyn cytology, all gyn cytology is sent out.







Thanks,



Mary K. Mullen, HTL(ASCP)CM
Histotechnologist
UPMC Northwest
Seneca, PA



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Re: [Histonet] Cytology/Histology Staining Question (Mullen, Mary)

[T H via Histonet]

Hey Mary,

The problem is not the machine, it is the reagents sharing that is the issue.  
You can use different reagents and protocols for the Histo and Cyto slides and 
on the same instrument.  You might even get away with changing your Alcohols 
and filtering everything else and see how that works.  Try it and run some 
blank slides through the stainer and see if anything is there from the cytology 
specimens.

I would personally have two separate sets of staining reagents to be on the 
safe side.

Good luck!!

Tim


Message: 7
Date: Tue, 10 May 2016 14:54:55 +
From: "Mullen, Mary" <mulle...@mail.magee.edu>
To: "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Subject: [Histonet] Cytology/Histology Staining Question
Message-ID:
<374dc72e6b29d44086f8ff3289351b2508823...@msxmbxnsprd39.acct.upmchs.net>

Content-Type: text/plain; charset="iso-8859-1"

Hello all,



I work in a small, low volume community hospital and was recently asked by a 
coworker why we do not just run both our cytology and histology slides on the 
same automated stainer (with their respective protocols).



What I am wanting to know is if there is anyone currently running both staining 
protocols on a single automated stainer using common alcohols/xylenes/water? 
What are the pros/cons? Has there been any cross-contamination issues?



We only run non-gyn cytology, all gyn cytology is sent out.







Thanks,



Mary K. Mullen, HTL(ASCP)CM
Histotechnologist
UPMC Northwest
Seneca, PA



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Re: [Histonet] Cytology/Histology Staining Question

[Rene J Buesa via Histonet]

I would be concerned with potential cross-contamination. In my lab we had 2 
staining instruments, one for cytology and other for histology.René 

On Tuesday, May 10, 2016 10:59 AM, "Mullen, Mary via Histonet" 
 wrote:
 

 Hello all,



I work in a small, low volume community hospital and was recently asked by a 
coworker why we do not just run both our cytology and histology slides on the 
same automated stainer (with their respective protocols).



What I am wanting to know is if there is anyone currently running both staining 
protocols on a single automated stainer using common alcohols/xylenes/water? 
What are the pros/cons? Has there been any cross-contamination issues?



We only run non-gyn cytology, all gyn cytology is sent out.







Thanks,



Mary K. Mullen, HTL(ASCP)CM
Histotechnologist
UPMC Northwest
Seneca, PA

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