There is a generally accepted scheme of staining which is expected of
Romanowsky stained preparations, namely purple chromatin, blue leucocyte
cytoplasm, purple-black basophil granules, red-pink eosinophil granules, purple
neutrophil granules, purple platelet granules, and pink red-cells (1).
According to recommendations of the French Society of Clinical Cytology and of
the French Association for Quality Assurance in Anatomic and Cytologic
Pathology (2), red blood cells and polymorphonuclear leukocytes are the target
cells for the quality evaluation of Romanowsky stains, and not the tumour
cells. The tinctorial quality of Romanowsky stains is better and reliably
judged on these target cells. Even if it is known that a ‘good MGG’ allows
pink–orange (acidophilic) or buff-coloured RBCs to be obtained, many
laboratories produce MGG slides showing blue or green erythrocytes, mainly
because of a high (alkaline) pH of dilution/rinse solutions (3).
Horobin (3) has provided a useful table to trouble-shoot Romanowsky staining.
1.Marshall PN, Bentley SA, Lewis SM. An evaluation of some commercial
Romanowsky stains. Journal of clinical pathology. 1975 Aug 1;28(8):680-5.
2.Piaton E, Fabre M, Goubin‐Versini I, Bretz‐Grenier MF, Courtade‐Saïdi M,
Vincent S, Belleannée G, Thivolet F, Boutonnat J, Debaque H, Fleury‐Feith J.
Guidelines for May‐Grünwald–Giemsa staining in haematology and
non‐gynaecological cytopathology: recommendations of the French Society of
Clinical Cytology (SFCC) and of the French Association for Quality Assurance in
Anatomic and Cytologic Pathology (AFAQAP). Cytopathology. 2016 Oct;27(5):359-68.
3. Horobin RW. How Romanowsky stains work and why they remain
valuable—including a proposed universal Romanowsky staining mechanism and a
rational troubleshooting scheme. Biotechnic & Histochemistry. 2011 Feb
1;86(1):36-51.
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principal Scientist, the Children’s Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
From: Estela Martinez via Histonet
Sent: Friday, 29 January 2021 02:53
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Dipp Kwik Stain
Hello All,
Do any of your pathologist use Dipp Kwik and if so, what kind of control do you
use and do you document the control? TIA!
Estela Martinez
Histology Supervisor
Medical Center Hospital
Odessa, TX 79761
432-640-2348
emartin...@echd.org
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