[Histonet] Disposal of Bouin's Solution
We use Bouin's Solution in our pathology department and are currently disposing of it by neutralizing it with Vytac for formalin. I can't find supporting documentation that this is the proper way to dispose of it. I'd like to know if and how other institutions are neutralizing Bouin's Solution. Thank you! Lori Jones, CT(ASCP) Pathology Supervisor Ingalls Memorial Hospital This message and any included attachments are from Ingalls Memorial Hospital and are intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail with a copy to mailsecur...@ingalls.org Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Disposal of Bouin's Solution
Lori Jones, CT(ASCP), Pathology Supervisor, Ingalls Memorial Hospital asks: >>We use Bouin's Solution in our pathology department and are currently disposing of it by neutralizing it with Vytac for formalin. I can't find supporting documentation that this is the proper way to dispose of it. I'd like to know if and how other institutions are neutralizing Bouin's solution.<< The best way to dispose of Bouin's fixative is by not buying it at all. What do you use it for? There are substitutes for it for most purposes. Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Disposal of Bouin's Solution
https://oag.ca.gov/sites/all/files/agweb/pdfs/cci/safety/picric.pdf Two hazards: formaldehyde and picric acid (see attached). While I agree with Dr. Richmond, changing protocols is not easily done. So. You may search for appropriate oxidizers of aldehydes. OR!!! You may take the reasonable path by sending it off periodically to a company that will cost enough to raise the use of the stuff to serious conversation. Picric acid - since I used it from the late 1950's - the end of our "Dark Ages"[???] - into the mid 1970's -the age when students became consumers and knowing what was on the test was paramount - IS really hazardous - when it is dry. [So, I always kept it covered with water (saturated in local conditions.] Picric acid is the reason that you should either stop using Bouin's or waste it (wet!!) to a competent waste-handling company. Cheers, Fred Monson (5 weeks to the oblivion called 'retirement.' -Original Message- From: Bob Richmond via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, May 11, 2017 6:39 PM To: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Disposal of Bouin's Solution Lori Jones, CT(ASCP), Pathology Supervisor, Ingalls Memorial Hospital asks: >>We use Bouin's Solution in our pathology department and are currently disposing of it by neutralizing it with Vytac for formalin. I can't find supporting documentation that this is the proper way to dispose of it. I'd like to know if and how other institutions are neutralizing Bouin's solution.<< The best way to dispose of Bouin's fixative is by not buying it at all. What do you use it for? There are substitutes for it for most purposes. Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Disposal of Bouin's Solution
Not a disposal query. "There are substitutes for it for most purposes." Bob, I am interested in "most" and "substitutes" In what situation does "most" not apply? What substitutes? For example, I read that tissues are fixed in Picric acid-containing Formalin for IHC/IF. Why? What's the logic/rationale? Always listening/reading/asking/learning Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Disposal of Bouin's Solution
I agree it is very dangerous. When I discovered a dried bottle of picric acid someone had donated to the school right after I first came to the university. I had to call the fire department and the bomb squad came to get it. They took it to the firing range and blew a huge sized whole in the ground, this was a small bottle of picric acid. Of course we made the local news, but get rid of it if possible or keep it under water as Fred says. Shirley -Original Message- From: Monson, Frederick via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Friday, May 12, 2017 2:16 PM To: Histonet@lists.utsouthwestern.edu Cc: Bob Richmond Subject: Re: [Histonet] Disposal of Bouin's Solution https://oag.ca.gov/sites/all/files/agweb/pdfs/cci/safety/picric.pdf Two hazards: formaldehyde and picric acid (see attached). While I agree with Dr. Richmond, changing protocols is not easily done. So. You may search for appropriate oxidizers of aldehydes. OR!!! You may take the reasonable path by sending it off periodically to a company that will cost enough to raise the use of the stuff to serious conversation. Picric acid - since I used it from the late 1950's - the end of our "Dark Ages"[???] - into the mid 1970's -the age when students became consumers and knowing what was on the test was paramount - IS really hazardous - when it is dry. [So, I always kept it covered with water (saturated in local conditions.] Picric acid is the reason that you should either stop using Bouin's or waste it (wet!!) to a competent waste-handling company. Cheers, Fred Monson (5 weeks to the oblivion called 'retirement.' -Original Message- From: Bob Richmond via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, May 11, 2017 6:39 PM To: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Disposal of Bouin's Solution Lori Jones, CT(ASCP), Pathology Supervisor, Ingalls Memorial Hospital asks: >>We use Bouin's Solution in our pathology department and are currently disposing of it by neutralizing it with Vytac for formalin. I can't find supporting documentation that this is the proper way to dispose of it. I'd like to know if and how other institutions are neutralizing Bouin's solution.<< The best way to dispose of Bouin's fixative is by not buying it at all. What do you use it for? There are substitutes for it for most purposes. Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Disposal of Bouin's Solution
"I agree it is very dangerous. When I discovered a dried bottle of picric acid someone had donated to the school right after I first came to the university. I had to call the fire department and the bomb squad came to get it. They took it to the firing range and blew a huge sized whole in the ground, this was a small bottle of picric acid. Of course we made the local news, but get rid of it if possible or keep it under water as Fred says. " Chucklewhy didn't you just wet it? It would only be "dangerous " if you hit it with a hammer The explosive used was FAR bigger than the bang from one bottle of Picric acid Overkill. The story about a factory involved in Picric acid explosion involves several magnitudes higher than any volume stored in a Lab In the volumes used in a laboratory.not really a problem unless used TOTALLY incompetently/maliciously Sureif incompetence is thereyou will get a bang akin to that one used to be able to buy from a Joke shop. FORMALIN is toxic, poisonous...it KILLS Don't use it! Do we stop using it? Hmmm In a well- run laboratory, I am FAR more concerned regarding the toxicity of stepping outside of my lab into the street where them cars are continuously spewing out far more toxic fumes. ALL OF THE TIME Yepalso the particulate matter from them car tyres that we breath in Does anyone regulate this? Nope! So, all you car drivers that are concerned re them small amounts of laboratory Picric acidthink again. Best wishes Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Disposal of Bouin's Solution
Hello, Regarding disposal of Bouin's solution the following protocol might be helpful/amusing/terrifying: https://phc.amedd.army.mil/PHC%20Resource%20Library/Bouins_fixative_solution_FS%20_37-007-0913.pdf For IHC the argument is that Bouin's doesn't destroy antigens as compared to other fixatives but I think this is a problem specific to certain antigens rather than a general issue---and those antigens can be preserved by other means now. It also seems to preserve nuclei much better. Bye -- Tyrone Genade Orange City, Iowa tel: (+1) 712 230 4101 http://tgenade.freeshell.org Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
