Re: [Histonet] dye in processor

[Bob Richmond via Histonet]

Jeff Halstead at Oregon Health & Science University asks:

"Some time ago info on the list discussed placing eosin or other dyes in
the processor and I was wondering what everyone is actually using. Heard
safranin o was a good idea . any thoughts?"

Safranin O - the solution your microbiology people use for Gram staining
works quite well - can be used to mark small pieces of tissue before
processing. It stains more densely than eosin, and unlike eosin it isn't
fluorescent. I worked in a lab that used this quite successfully. They
placed the small specimens on those blue pads before marking them with a
drop of dye, processing through xylene (or aliphatic substitute - I don't
remember) on a truly ancient Fisher processor. Both they and I liked the
results.

Bob Richmond
Samurai Pathologist
Maryville TN
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[Histonet] dye in processor

[Jeff Halstead via Histonet]

Hi all some time ago info on the list discussed placing eosin or other dyes in 
the processor and I was wondering what everyone is actually using. Heard 
safranin o was a good idea . any thoughts. Thanx jeff
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Re: [Histonet] Dye ?

[Michael Ann Jones via Histonet]

Is it possible that the clinician is using some sort of dye during
collection?

Michael Ann
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com







On 9/25/15, 9:23 AM, "Melissa Burns via Histonet"
 wrote:

>Hello All-
>
>I'm having an ongoing issue that I'm hoping all of you smart people can
>help me with! We are talking about prostate needle biopsy specimens.
>
>We have one company that does a genetic test that is insisting that there
>is a dye present in the tissue we are sending. None of the other genetics
>companies we send to have had an issuejust this oneand not on
>every case we send them! Imagine my confuse :)
>
>The specimens are sent in 10% NBF. There is no dye used in grossing or
>processing.
>
>Am I missing something? Somewhere that dye may be sneaking in?
>
>We are to the point now that they want to come to the lab and the
>collecting surgery centers and see if they can figure it out.
>
>Baffled
>
>Melissa
>
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[Histonet] Dye ?

[Melissa Burns via Histonet]

Hello All-

I'm having an ongoing issue that I'm hoping all of you smart people can help me 
with! We are talking about prostate needle biopsy specimens.

We have one company that does a genetic test that is insisting that there is a 
dye present in the tissue we are sending. None of the other genetics companies 
we send to have had an issuejust this oneand not on every case we send 
them! Imagine my confuse :)

The specimens are sent in 10% NBF. There is no dye used in grossing or 
processing.

Am I missing something? Somewhere that dye may be sneaking in?

We are to the point now that they want to come to the lab and the collecting 
surgery centers and see if they can figure it out.

Baffled

Melissa

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