Since EDTA is essentially an aqueous solution, I would wash out the 70EthOL,
fix in NBF for 24 hours and then place the specimen in EDTA until
decalcification is completed.
René J.
--- On Wed, 6/22/11, Orla M Gallagher o.m.gallag...@sheffield.ac.uk wrote:
From: Orla M Gallagher o.m.gallag...@sheffield.ac.uk
Subject: [Histonet] EDTA decalcification of bones which have been fixed in 70%
ethanol
To: histonet@lists.utsouthwestern.edu
Date: Wednesday, June 22, 2011, 7:55 AM
Dear Histonetters,
We would like to decalcify some mouse bones in EDTA pH 7 which have been
received by our lab already fixed directly in 70% ethanol rather than in
formalin or paraformaldehyde.
How would you recommend preparing these bones for decalcification e.g.
whether to post-fix in formalin or to wash out the ethanol before
transferrring to EDTA? I realise that ethanol is not the best fixative to
use, especially as the end user may want to do immunocytochemistry or enzyme
histochemistry using TRAP.
Thanks,
Orla
--
**
Ms. Orla Gallagher
Bone Analysis Laboratory
Mellanby Centre for Bone Research
D Floor Medical School
University of Sheffield
Beech Hill Road
Sheffield
S10 2RX
Website: http://mellanbycentre.dept.shef.ac.uk
Tel: 0114-2713337 (office)
0114-2713174 (lab)
E-Mail: o.m.gallag...@sheffield.ac.uk
Please think about the environment before printing this email
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
