From: Rizaldy P Scott <rizaldy.sc...@northwestern.edu> Sent: Wednesday, April 10, 2024 4:33 PM To: Bernice Frederick <b-freder...@northwestern.edu> Subject: Re: [Histonet] Golgi-cox staining
Hi Bernice, What they should have done ideally is to store the uncut agarose blocks submerged in whatever buffer they used to rinse the brains out prior to agarose embedding. They can probably salvage the dried-out brains by rehydrating in their buffer or 30% sucrose solution overnight (or store at 4 degrees as needed) and re-embed in agarose only when they are ready to cut. I can't guarantee however if artefacts due to drying might result but it's worth the shot. Best regards, Riz Rizaldy P. Scott, M.S., Ph.D. Research Associate Professor of Pathology Scientific Director Mouse Histology & Phenotyping Laboratory Robert H. Lurie Comprehensive Cancer Center Northwestern University Feinberg School of Medicine 📍Olson Pavilion, Room 8-335, 710 N. Fairbanks Ct., Chicago, IL 60611 ✉️ rizaldy.sc...@northwestern.edu<mailto:rizaldy.sc...@northwestern.edu> 📞 +1-(312)-503-2695 https://www.feinberg.northwestern.edu/sites/mhpl/ www.cancer.northwestern.edu<http://www.cancer.northwestern.edu> ________________________________ From: Bernice Frederick <b-freder...@northwestern.edu<mailto:b-freder...@northwestern.edu>> Sent: Wednesday, April 10, 2024 13:40 To: Rizaldy P Scott <rizaldy.sc...@northwestern.edu<mailto:rizaldy.sc...@northwestern.edu>> Subject: FW: [Histonet] Golgi-cox staining Can you help this person? Bernice -----Original Message----- From: Mariela Chertoff via Histonet <histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>> Sent: Wednesday, April 10, 2024 9:28 AM To: histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu> Subject: [Histonet] Golgi-cox staining Hi all We made the Golgi cox staining and due to a problem with the vibratome, we left the tissue several days embedded in agarose and they get dryed, It is possoble to recover the brains? it is better to repeat the agarose embebbing o it is better to put the brains in crioprerervate solution to rehidrated and after that put them in agarosa again? We are following the Zaquot protocol https://urldefense.com/v3/__https://www.frontiersin.org/articles/10.3389/fnana.2016.00038/full__;!!Dq0X2DkFhyF93HkjWTBQKhk!QkwV4_RJl5cyhElJgSA-8lRMmOSmkwVoHzRrMg8-ZXGX0yUzNCxM_pm09t45KaZWo04kpjD-tBduP59_0DHjxeEvlRMxvl848hoVaCzp$<https://urldefense.com/v3/__https:/www.frontiersin.org/articles/10.3389/fnana.2016.00038/full__;!!Dq0X2DkFhyF93HkjWTBQKhk!QkwV4_RJl5cyhElJgSA-8lRMmOSmkwVoHzRrMg8-ZXGX0yUzNCxM_pm09t45KaZWo04kpjD-tBduP59_0DHjxeEvlRMxvl848hoVaCzp$> Thanks in advance for your reply Mariela Chertoff, PhD Laboratorio de Neuroepigenetica - QB75 Departamento de Química Biológica Facultad de Ciencias Exactas y Naturales - UBA Ciudad Universitaria Pabellón II Piso 4 Ciudad Autónoma de Buenos Aires C1428EGA - Argentina Tel: 54 11 5285-8680/1/2 email:marielachert...@gmail.com marielachert...@qb.fcen.uba.ar<mailto:marielachert...@qb.fcen.uba.ar> <mariela.chert...@uab.cat<mailto:mariela.chert...@uab.cat>> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu> https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!Dq0X2DkFhyF93HkjWTBQKhk!QkwV4_RJl5cyhElJgSA-8lRMmOSmkwVoHzRrMg8-ZXGX0yUzNCxM_pm09t45KaZWo04kpjD-tBduP59_0DHjxeEvlRMxvl848ty2UFsq$<https://urldefense.com/v3/__http:/lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!Dq0X2DkFhyF93HkjWTBQKhk!QkwV4_RJl5cyhElJgSA-8lRMmOSmkwVoHzRrMg8-ZXGX0yUzNCxM_pm09t45KaZWo04kpjD-tBduP59_0DHjxeEvlRMxvl848ty2UFsq$> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
