Re: [Histonet] Fibroblasts

[Elizabeth Chlipala via Histonet]

Bernice

There are lots of different markers for fibroblasts via IHC - If you are 
looking for a good source Acris Antibodies has quite a few fibroblast markers

Vimentin, 
Smooth Muscle Actin
FSP-1 (fibroblast specific protein)
Pro Collagen I 
CD34
Prolyl-4-Hydroxylase beta
HSP-47

I have a two page document that I will send to you that lists a lot of what I 
have already and what types of fibroblasts that they stain for - we have used 
most of these in various species.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
l...@premierlab.com
www.premierlab.com

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504


-Original Message-
From: Bernice Frederick via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Wednesday, January 04, 2017 1:02 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Fibroblasts

Hello all,
Don't have a ton of time to search so am asking. Any good markers specific to 
fibroblasts? And am drawing a blank for regular special stains for some reason. 
Researcher says Vimentin is not specific enough.
Thanks,
Bernice

Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-freder...@northwestern.edu<mailto:b-freder...@northwestern.edu>

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[Histonet] Fibroblasts

[Bernice Frederick via Histonet]

Hello all,
Don't have a ton of time to search so am asking. Any good markers specific to 
fibroblasts? And am drawing a blank for regular special stains for some reason. 
Researcher says Vimentin is not specific enough.
Thanks,
Bernice

Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-freder...@northwestern.edu

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[Histonet] Fibroblasts Floating on Medium after 24 hrs.

[Khaire Dai]

Hi
 
I have started growing the human normal and cancer associated fibroblasts.
 
The cells I got are from the vials preserved in liquid nitrogen and have seeded 
the cells on culture plates. Now after 24 hrs only very few cells are attached 
to the plate almost all of them are floating.
 
How long should i wait for the cells to attach or remove the floating cells?
I don't want to loose them as i dont have any backup for these cells.
 
Thanks in advance for the support.
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