We regularly leave smeared slides in 95% overnight and often over the weekend before staining. There have not been any morphological issues noted. Remember to seal the lid since alcohol will evaporate quite quickly. You will notice an increased eosinophilia in the dried upper area of the slide. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children’s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA ________________________________________ From: Perl, Alison via Histonet <histonet@lists.utsouthwestern.edu> Sent: Saturday, 14 March 2020 04:19 To: 'Charles Riley via Histonet' Subject: Re: [Histonet] [EXTERNAL] Pap slides We routinely leave our ThinPrep Paps in 95 overnight and stain the next day. If I remember our Hologic training correctly, they said the slides can be left up to 5 days - best to check with an FAS before trying it, though Alison Perl, HTL(ASCP)CM Anatomic Pathology Manager CareMount Medical (914) 302-8424 ap...@caremount.com -----Original Message----- From: Charles Riley via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Friday, March 13, 2020 12:52 PM To: Histo List Subject: [EXTERNAL] [Histonet] Pap slides Can pap slides be left in 95% alcohol overnight to be stained the next morning without affecting the quality of the slides? Or should they be moved to xylene then run back prior to staining? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fw: [EXTERNAL] Pap slides
Tony Henwood (SCHN) via Histonet Sat, 14 Mar 2020 14:04:02 -0700
- [Histonet] Pap slides Charles Riley via Histonet
- Re: [Histonet] [EXTERNAL] Pap slide... Perl, Alison via Histonet
- [Histonet] Fw: [EXTERNAL] Pap ... Tony Henwood (SCHN) via Histonet
- Re: [Histonet] [EXTERNAL Sender] Pa... Hannen, Valerie via Histonet