Hi Clay, The first solution is methanol. So lab grade methanol will do. The blue colour is from an innocuous dye added to differentiate from water (and ethanol used in cytology fixation). This helps us poor cytologists when we are doing ROSE at FNAs.
Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children’s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Corbin, Clay via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Tuesday, 5 October 2021 7:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fixative in diff-quick Hey folks, I am shopping for a diff-quick kit. However, all I really need is the fixative. Generally, there is a blue stain (triarylmethane) added to the methanol in the fixative solution. I have a giant jug of lab grade methanol. What would I lose by using methanol alone compared to the fixative solution included in a diff-quick kit? Thanks! Clay Clay Corbin, PhD Professor of Biology Bloomsburg University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
